Congratulations to the Libri Lab for this new paper published in Molecular Cell! Sen1 is a key regulator of transcription-driven conflicts   Summary Cellular homeostasis requires the coordination of several machineries concurrently engaged in the DNA. Wide-spread transcription can interfere with other processes, and transcription-replication conflicts (TRCs) threaten genome stability. The conserved Sen1 helicase not only terminates non-coding transcription…

Just published in STAR Protocols! Congratulations to Borghi Lab! Molecular tension microscopy of the LINC complex in live cells   Summary We present a protocol to measure the effect of pharmacological treatments on the mechanical tension experienced by nesprins at the cytoplasmic surface of the nuclear envelope of mammalian cells in culture. We apply this protocol to…

Congratulations to Romet-Lemonne / Jégou Team for this new paper just published in JoVE: Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles Abstract In order to decipher the complex molecular mechanisms that regulate the assembly and disassembly of actin filaments, it is a great asset to monitor individual…

Congratulations to the Ribes Team for this new paper just published in PLOS Genetics!   Divergent transcriptional and transforming properties of PAX3-FOXO1 and PAX7-FOXO1 paralogs   Abstract The hallmarks of the alveolar subclass of rhabdomyosarcoma are chromosomal translocations that generate chimeric PAX3-FOXO1 or PAX7-FOXO1 transcription factors. Overexpression of either PAX-FOXO1s results in related cell transformation in animal models. Yet,…