The Ladoux/Mège Team has just published a new paper in Science Advances: The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces   Abstract Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous…

The Plessis Lab has just published a new paper in eLife! High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothened Abstract The oncogenic G-protein-coupled receptor (GPCR) Smoothened (SMO) is a key transducer of the hedgehog (HH) morphogen, which plays an essential role in the patterning of epithelial structures.…

A new paper just published by Léon Lab in PLOS Genetics! 2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity   Abstract 2-deoxyglucose is a glucose analog that impacts many aspects of cellular physiology. After its uptake and its phosphorylation into 2-deoxyglucose-6-phosphate (2DG6P), it interferes with several metabolic pathways including glycolysis and…

A new article co-authored by the ProtéoSeine Facility:   Cisplatin causes covalent inhibition of protein-tyrosine phosphatase 1B (PTP1B) through reaction with its active site cysteine: Molecular, cellular and in vivo mice studies Abstract Protein tyrosine phosphatase 1B (PTP1B) is a critical regulator of different signalling cascades such as the EGFR pathway. The biological importance of PTP1B is further evidenced…

A new review just published in Insects by Manon Monier et Virginie Courtier: Drosophila Glue: A Promising Model for Bioadhesion Abstract The glue produced by Drosophila larvae to attach themselves to a substrate for several days and resist predation until the end of metamorphosis represents an attractive model to develop new adhesives for dry environments. The adhesive…

Congratulations to the Libri Lab for this new paper published in Molecular Cell! Sen1 is a key regulator of transcription-driven conflicts   Summary Cellular homeostasis requires the coordination of several machineries concurrently engaged in the DNA. Wide-spread transcription can interfere with other processes, and transcription-replication conflicts (TRCs) threaten genome stability. The conserved Sen1 helicase not only terminates non-coding transcription…

Just published in STAR Protocols! Congratulations to Borghi Lab! Molecular tension microscopy of the LINC complex in live cells   Summary We present a protocol to measure the effect of pharmacological treatments on the mechanical tension experienced by nesprins at the cytoplasmic surface of the nuclear envelope of mammalian cells in culture. We apply this protocol to…

Congratulations to Romet-Lemonne / Jégou Team for this new paper just published in JoVE: Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles Abstract In order to decipher the complex molecular mechanisms that regulate the assembly and disassembly of actin filaments, it is a great asset to monitor individual…