Facilities and services
Flow cytometry
Flow cytometry is a high-output technique that allows quantitative multiparametric analysis (light scattering measurments, fluorescence properties) at the single-cell level.
>> Flow cytometers (Analyzers and sorter)
Electronic microscopy
Methods available:
Equipment:
- Tecnai 12 trasnmission electron microscope equipped with a CCD camera (OneView 4Kx4K Gatan) driven by the GMS software (self-service after training)
- TeneoVS scanning electron microscope, equipped with ETD, SE and BSE detectors and the VolumeScope module (for Serial-Block-Face mode) driven by FEI and Maps2 software (self-service after training)
- Fischione 2020 tomography object holder, with tilt up to +/-80°.
- Leica EM PACT2 high pressure cryofixation unit
- Leica AFS devices: automated cryosubstitution.
- ultramicrotomes (1 Leica UC6, 1 Leica UCT): production of semi- and ultra-fine sections of biological samples embedded in various resins (self-service after training).
- Leica UC7-FC7 ultracryomicrotome: making semi- and ultra-fine sections of frozen biological samples.
- Cressington 308R carbon evaporator: vacuum evaporation of carbon on grids (self-service after training).
Photonic microscopy
Plein champ
Principe, équipements disponibles
Spinning disk
Principe, équipements disponibles
Confocal à balayage laser
Principe, équipements disponibles
Super résolution SIM, PALM, STORM
Principe, équipements disponibles
Multi-photon, SHG, THG
Principe, équipements disponibles en 2022
FLIM, FCS, PIE
Principe, équipements disponibles en 2022
Image processing and analysis
The Platform provides help and assistance to users for image processing, analysis, quantification and interpretation.
Available software:
Imaris (Bitplane) : software for reconstruction and representation of 3-4-5 D images, and for image quantification and manipulation; it is interfaced with :
Fiji :
- Website ImageJ : http://imagej.nih.gov/ij
- Website Fiji : http://fiji.sc/Fiji
- Wiki on ImageJ and Fiji : http://imagejdocu.tudor.lu
and
Matlab : Mathworks Website : www.mathworks.fr
Amira (FEI): software for reconstruction and representation of 3-4-5 D images, and for image quantification and manipulation.
Website FEI : www.fei.com/software/amira-3d-for-life-sciences.
Matlab (MathWorks) : Digital calculation programming software allowing the development of advanced processing (image processing, signal processing, statistics, etc.) on images. It can be interfaced with the software Imaris.
Website Mathworks : www.mathworks.fr
Huygens Pro (SVI – Scientific Volume Imaging) : image deconvolution software; it allows the deflowering and denoising of images based on the knowledge of the physical and optical characteristics of the microscopes and the sample.
Website SVI : www.svi.nl
ImageJ et Fiji: free software for processing and analysing 3-4-5 D images with numerous plugins and allowing the creation of custom scripts (macros).
- Website ImageJ : http://imagej.nih.gov/ij
- Website Fiji : http://fiji.sc/Fiji
- Wiki on ImageJ and Fiji : http://imagejdocu.tudor.lu
Icy : Free software for processing and analysing 3-4-5 D images with many plugins and allowing the creation of custom scripts (macros).
Website : icy.bioimageanalysis.org
CellProfiler: free software for the quantification of large image series.
Website : www.cellprofiler.org
Zen (Zeiss) : image manipulation software allowing the opening of images with the same interface as with the acquisition software of Zeiss microscopes; it also allows image analysis
Website Zen desk (Zeiss) : www.zeiss.com/zen
Website Zen lite (Zeiss) : www.zeiss.com/zen-lite
Metamorph Offline (Molecular Devices) : image manipulation software allowing the opening of images with the same interface as with the Metamorph acquisition software; it also allows image analysis.
Website Metamorph : www.moleculardevices.com/products/software/meta-imaging-series/metamorph.html
Workstations
The software is installed on 2 powerful workstations.
- Station 1 Imaris
Processeur : - Station 3 Imaris
Double processeur : 3,47 GHz, 12 cœurs physiques ; mémoire : 144 Go ; carte graphique : 6 Go.
A graphics tablet is also available, facilitating manual image segmentation operations.
