Facilities and services

 

 

Flow cytometry

Flow cytometry is a high-output technique that allows quantitative multiparametric analysis (light scattering measurments, fluorescence properties) at the single-cell level.

 

Electronic microscopy

 

Methods available:

 

Equipment:

  • Tecnai 12 trasnmission electron microscope equipped with a CCD camera (OneView 4Kx4K Gatan) driven by the GMS software (self-service after training)
  • TeneoVS scanning electron microscope, equipped with ETD, SE and BSE detectors and the VolumeScope module (for Serial-Block-Face mode) driven by FEI and Maps2 software (self-service after training)
  • Fischione 2020 tomography object holder, with tilt up to +/-80°.
  • Leica EM PACT2 high pressure cryofixation unit
  • Leica AFS devices: automated cryosubstitution.
  • ultramicrotomes (1 Leica UC6, 1 Leica UCT): production of semi- and ultra-fine sections of biological samples embedded in various resins (self-service after training).
  • Leica UC7-FC7 ultracryomicrotome: making semi- and ultra-fine sections of frozen biological samples.
  • Cressington 308R carbon evaporator: vacuum evaporation of carbon on grids (self-service after training).

 

Photonic microscopy

 

Plein champ
Principe, équipements disponibles

Spinning disk
Principe, équipements disponibles

Confocal à balayage laser
Principe, équipements disponibles

Super résolution SIM, PALM, STORM
Principe, équipements disponibles

Multi-photon, SHG, THG
Principe, équipements disponibles en 2022

FLIM, FCS, PIE
Principe, équipements disponibles en 2022

 

Image processing and analysis

 

The Platform provides help and assistance to users for image processing, analysis, quantification and interpretation.

 

Available software:

 

Imaris (Bitplane) : software for reconstruction and representation of 3-4-5 D images, and for image quantification and manipulation; it is interfaced with :

Fiji :

and

Matlab : Mathworks Website : www.mathworks.fr

 

Amira (FEI): software for reconstruction and representation of 3-4-5 D images, and for image quantification and manipulation.

Website FEI : www.fei.com/software/amira-3d-for-life-sciences.

 

Matlab (MathWorks) : Digital calculation programming software allowing the development of advanced processing (image processing, signal processing, statistics, etc.) on images. It can be interfaced with the software Imaris.

Website Mathworks : www.mathworks.fr

 

Huygens Pro (SVI – Scientific Volume Imaging) : image deconvolution software; it allows the deflowering and denoising of images based on the knowledge of the physical and optical characteristics of the microscopes and the sample.

Website SVI : www.svi.nl

 

ImageJ et Fiji: free software for processing and analysing 3-4-5 D images with numerous plugins and allowing the creation of custom scripts (macros).

 

Icy : Free software for processing and analysing 3-4-5 D images with many plugins and allowing the creation of custom scripts (macros).

Website : icy.bioimageanalysis.org

 

CellProfiler: free software for the quantification of large image series.

Website : www.cellprofiler.org

 

Zen (Zeiss) : image manipulation software allowing the opening of images with the same interface as with the acquisition software of Zeiss microscopes; it also allows image analysis

Website Zen desk (Zeiss) : www.zeiss.com/zen

Website Zen lite (Zeiss) : www.zeiss.com/zen-lite

 

Metamorph Offline (Molecular Devices) : image manipulation software allowing the opening of images with the same interface as with the Metamorph acquisition software; it also allows image analysis.

Website Metamorph : www.moleculardevices.com/products/software/meta-imaging-series/metamorph.html

 

Workstations

The software is installed on 2 powerful workstations.

  • Station 1 Imaris
    Processeur :
  • Station 3 Imaris
    Double processeur : 3,47 GHz, 12 cœurs physiques ; mémoire : 144 Go ; carte graphique : 6 Go.

A graphics tablet is also available, facilitating manual image segmentation operations.