Proteomics: Mass Spectrometry

ProteoSeine is the proteomics facility of the Institut Jacques Monod, UMR 7592 Université de Paris Cité – CNRS. It has been located since early 2009 in the Buffon building at 15 rue Hélène Brion, 75205 Paris CEDEX 13 – France. The platform’s activities include analytical services, technical and methodological research and development, and active technology watch. In this context, it uses the most modern approaches of mass spectrometry for the study of protein constituents of living systems.

Facilities

Its mission is to offer the biology research community state-of-the-art skills and services to integrate proteomic approaches into the development of their scientific projects.

ProteoSeine’s R&D is based on the active research in fundamental biology of the Institut Jacques Monod teams, on collaborations with the hospital sector and on a long-standing know-how in the analytical development of therapeutic proteins. The team is particularly developing its expertise in the high-throughput differential quantitative analysis of complex protein mixtures, the analysis of post-translational modifications, the characterisation of recombinant pharmaceutical proteins and the analysis of non-covalent complexes by mass spectrometry under native conditions. Bottom-up and top-down approaches at the nano or capillary scale are used according to the problems encountered.

The platform is labelled by the GIS IBiSA (Infrastructure for Biology, Health and Agronomy) and by the Cancéropôle Ile-de-France. It is open to the entire national and international, public and private scientific community. The traceability of activities is ensured through the registration of services and users in a LIMS specially developed internally and raw data are stored in redundancy via a solution managed globally by the Institut Jacques Monod. ProteoSeine is also an associate member of ProFi, the national research infrastructure dedicated to proteomic analysis and is supported by the following organisations:

Equipments

ProteoSeine implements high-resolution electrospray mass spectrometers coupled to high-performance chromatographic systems.

Bottom-up analyses are performed on a latest generation timsTOF Pro2 (Bruker) coupled to a high-throughput separation instrument (Evosep One). The TIMS (Trapped Ion Mobility Spectrometry) technology is primarily a gas phase separation technique that solves sample complexity by adding an extra dimension of separation to high performance liquid chromatography and mass spectrometry. The TIMS device also accumulates and concentrates ions of a given m/z and mobility. By synchronising the ion accumulation and sequencing cycles in parallel (Parallel Accumulation-Serial Fragmentation (PASEF) technology), the device can offer extremely high MS/MS speed and sensitivity for in-depth proteome coverage and unbiased differential protein quantification.

The platform is also equipped with proprietary software that allows reprocessing of raw data on two multi-core computing servers and multivariate statistical analysis of abundance results. The facility is in particularly good capacity to handle large sample sizes in automated workflows.

Native-MS and intact protein analyses are performed on a Cyclic IMS instrument (Waters) incorporating a revolutionary cyclic ion mobility technology. The instrument also has EXD and SID fragmentation capabilities in addition to the CID fragmentation mode available in the TRAP and TRANSFER cells. This instrument is coupled to a high-pressure capillary chromatographic system of the M-Class type or Acquity Premier for the development of high-resolution protein separations. An ADVION nanomate robot is also available for automated direct infusion analysis in nanospray mode.

ProteoSeine implements also a Hamilton liquid handler for automated sample preparation and a high-pressure chromatographic system for handling protein separations in different modalities.

Services

The facility implements high-resolution electrospray mass spectrometers coupled to high-performance chromatographic systems. The instrumentation and bioinformatics tools used are specially selected to deliver state-of-the-art results in proteomic analysis.

Various analysis protocols are proposed by analysing intact proteins (top-down) or after digestion (bottom-up), which make it possible to tackle the following problems in particular:

Identification of proteins on gel or in solution
Characterisation of post-translational modifications (PTMs)
Characterisation of recombinant proteins (coverage, PTMs, process & product-related impurities, size or charge variants)
Search for partners (Pull-Down)
Biomarker research
Label-free quantitative analysis of proteome abundance variations
Analysis of specific proteomes after enrichment (e.g. phosphoproteome)
Analysis of complexes by MS under native conditions

The principle of operation is to first contact the platform to define an analysis protocol adapted to the project. A user sheet specifying how the platform works will be sent (also available in the “documents” section). Prior to any analysis, this form must be returned completed with the requested information. In a second step, it is sufficient to make an appointment to drop off the samples from Tuesday to Thursday in order for us to be able to process them as soon as possible.

The results generated are sent by e-mail within 2 weeks for classic approaches and are archived. The raw data are transmitted on demand as their storage and integrity are ensured by the platform in a non-contractual way. After receiving the results, the user is therefore responsible for the integrity of all his data.

Pricing

The pricing of the facility’s services is calculated on the basis of full costs according to the CNRS DSFIM-SBOR-D-2014-46 reference system. Three distinct rates are applied according to the origin of the issuer of the order form, i.e. CNRS/UPC, other academic and private. The rates below are expressed exclusive of tax; they are to be increased by VAT at the current.

The mixed UPC/INSERM units under global INSERM management delegation listed below, nevertheless benefit from the CNRS/UPC rate.

UMR	Directeur	Intitulé	Implantation
U942	Alain Cohen-Solal	Biomarqueurs cardio-neuro-vasculaires : BIO-CANVAS	Hôpital Lariboisière
U958	Cécile Julier	Génétique des diabètes	Faculté médecine UP
U965	Marc Pocard	CARCINOSE angiogenèse et recherche transactionnelle : CART	Hôpital Lariboisière
U1131	Stephane GIRAUDIER	Hématopoïèse normale et pathologique : émergence, environnement et recherche translationnelle	Hôpital Saint-Louis
U976	Armand Bensussan	HIPI « Immunologie Humaine, Pathophysiologie, Immunothérapie »	Hôpital Saint-Louis
U1160	Antoine Toubert	Écotaxie, micro-environnement et développement lymphocytaire	Hôpital Saint-Louis
U944	Jean Soulier	Génomes, biologie cellulaire et thérapeutique	Hôpital Saint-Louis
U1123	Corinne Alberti	Epidémiologie clinique et évaluation économique appliquées aux populations vulnérables : ECEVE	Faculté médecine UP
U1132	Martine Cohen-Solal	Biologie de l’os et du cartilage : régulations et ciblage thérapeutique : BIOSCAR	Hôpital Lariboisière
U1134	Yves Collin	Biologie intégrée du globule rouge	INTS
U1137	Erik Denamur	Infection, antimicrobiens, modélisation, évolution : IAME	Faculté Bichat
U1141	Pierre Gressens	Neuroprotection du cerveau en développement : PROTECT	Hôpital Robert Debré
U1148	Didier Letourneur	Laboratoire de recherche vasculaire transactionnelle	Faculté Bichat
U1149	Renato Monteiro	Centre de recherche sur l’inflammation : CRI	Faculté Bichat
U1152	Marina Pretolani	Physiopathologie et épidémiologie des maladies respiratoires	Faculté Bichat
U1161	Elisabeth Tournier-Lasserve	Génétique et physiopathologie des maladies cérébro-vasculaires	Faculté médecine UP

Training

The ProteoSeine team is heavily involved in initial and continuing education activities.

Each year, it provides a week of theoretical and practical training in proteomic analysis by mass spectrometry to students in the M2-Pro “Platform Engineering” at Université Paris Cité.

Every year, it hosts an M2-level student for a 6-month practical end-of-studies internship.

It organises training in mass spectrometry in quantitative proteomics and characterisation of recombinant proteins. This training is listed in the CNRS Formation Entreprise catalogue.

It organises two international workshops per year as part of the European Twinning H2020 programme “MiCoBion”.

Contact and access

To contact the facility:

spectrodemasse@ijm.fr, +33 (0)1 57 27 81 82

Guillaume Chevreux	Head of Facility	guillaume.chevreux@ijm.fr +33 (0)1 57 27 81 19 orcid.org/0000-0003-3452-2125
Jean-Michel Camadro	Scientific coordinator	jean-michel.camadro@ijm.fr orcid.org/0000-0002-8549-2707
Véronique Legros	Research Engineer	veronique.legros@ijm.fr orcid.org/0000-0002-2502-2914
Victor Cochard	Engineer	victor.cochard@ijm.fr
Marie Ley	Postdoc	marie.ley@ijm.fr

ProteoSeine facility of Institut Jacques Monod, Bâtiment Buffon – Aile B, Pièce 657B, 6^e étage – 15 rue Hélène Brion – 75205 Paris Cedex 13 – FRANCE

Documentation

User’s charter

4202101_Fiche Utilisateur ProteoSeine_2022

Minimising contamination for proteomic analysis

Minimizing contamination in Proteomics

Cell lysis protocols for proteomic analysis

Preparing cell lysates for proteomics

RIPA Lysis

Publications

Publications in co-authorship with members of the facility (2017-2023)

2023

Lignieres L, Sénécaut N, Dang T, Bellutti L, Hamon M, Terrier S, Legros V, Chevreux G, Lelandais G, Mège RM, Dumont J, Camadro JM. Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling. J Proteome Res. 2023 Mar 3;22(3):996-1002. doi: 10.1021/acs.jproteome.2c00699. Epub 2023 Feb 6. PMID: 36748112; PMCID: PMC9990122.

kombo Nkoula S, Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Ayuso C, Legros V, Chevreux G, Thomas L, Seydoux G, Askjaer P, Pintard L. Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in C. elegans embryos. Sci Adv. 2023 Jul 21;9(29):eadf7826. doi: 10.1126/sciadv.adf7826. Epub 2023 Jul 19. PMID: 37467327; PMCID: PMC10355831.

Trouillard O, Dupaigne P, Dunoyer M, Doulazmi M, Herlin MK, Frismand S, Riou A, Legros V, Chevreux G, Veaute X, Busso D, Fouquet C, Saint-Martin C, Méneret A, Trembleau A, Dusart I, Dubacq C, Roze E. Congenital mirror movements are associated with defective polymerisation of RAD51. J Med Genet. 2023 Jun 12:jmg-2023-109189. doi: 10.1136/jmg-2023-109189. PMID: 37308287.

Nashed S, El Barbry H, Benchouaia M, Dijoux-Maréchal A, Delaveau T, Ruiz-Gutierrez N, Gaulier L, Tribouillard-Tanvier D, Chevreux G, Le Crom S, Palancade B, Devaux F, Laine E, Garcia M. Functional mapping of N-terminal residues in the yeast proteome uncovers novel determinants for mitochondrial protein import. PLoS Genet. 2023 Aug 16;19(8):e1010848. doi: 10.1371/journal.pgen.1010848. PMID: 37585488.

Gareil N, Gervais A, Macaisne N, Chevreux G, Canman JC, Andreani J, Dumont J. An unconventional TOG domain is required for CLASP localization. Curr Biol. 2023 Jul 21:S0960-9822(23)00916-8. doi: 10.1016/j.cub.2023.07.009. PMID: 37516114.

Zheng Y, Cabassa-Hourton C, Eubel H, Chevreux G, Lignieres L, Crilat E, Braun HP, Lebreton S, Savouré A. Pyrroline-5-carboxylate metabolism protein complex detected in Arabidopsis thaliana leaf mitochondria. J Exp Bot. 2023 Oct 16:erad406. doi: 10.1093/jxb/erad406. PMID: 37843921.

Postic, G., Solarz, J., Loubière, C., Kandiah, J., Sawmynaden, J., Adam, F., Vilaire, M., Léger, T., Camadro, J.-M., Victorino, D. B., Potier, M.-C., Bun, E., Moroy, G., Kauskot, A., Christophe, O., & Janel, N. (2023). Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice. Journal of Cellular and Molecular Medicine, n/a(n/a). https://doi.org/10.1111/jcmm.17817

Scrima N, Le Bars R, Nevers Q, Glon D, Chevreux G, Civas A, Blondel D, Lagaudriere-Gesbert C, Gaudin Y (2023) Rabies virus P protein binds to TBK1 and interferes with the formation of innate immunity-related liquid condensates. Cell Rep. 42: 111949, doi: S2211-1247(22)01850-2, PMID: 36640307

Bayot J, Martin C, Chevreux G, Camadro JM, Auchere F (2023) The adaptive response to alternative carbon sources in the pathogen Candida albicans involves a remodeling of thiol-and glutathione-dependent redox status. Biochem J. doi: BCJ20220505, PMID: 36625375

Lignieres L, Legros V, Khelil M, Senecaut N, Lauber MA, Camadro JM, Chevreux G (2023) Capillary liquid chromatography coupled with mass spectrometry for analysis of nanogram protein quantities on a wide-pore superficially porous particle column in top-down proteomics. J Chromatogr B Analyt Technol Biomed Life Sci. 1214: 123566, doi: S1570-0232(22)00471-8, PMID: 36516651

2022

Gallo G, Kotlik P, Roingeard P, Monot M, Chevreux G, Ulrich RG, Tordo N, Ermonval M (2022) Diverse susceptibilities and responses of human and rodent cells to orthohantavirus infection reveal different levels of cellular restriction. PLoS Negl Trop Dis. 16: e0010844, doi: 10.1371/journal.pntd.0010844, PMID: 36223391

Le Borgne P, Greibill L, Laporte MH, Lemullois M, Bouhouche K, Temagoult M, Rosnet O, Le Guennec M, Lignieres L, Chevreux G, Koll F, Hamel V, Guichard P, Tassin AM (2022) The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly. PLoS Biol. 20: e3001782, doi: 10.1371/journal.pbio.3001782, PMID: 36070319

Senecaut N, Poulain P, Lignieres L, Terrier S, Legros V, Chevreux G, Lelandais G, Camadro JM (2022) Quantitative Proteomics in Yeast: From bSLIM and Proteome Discoverer Outputs to Graphical Assessment of the Significance of Protein Quantification Scores. Methods Mol Biol. 2477: 275-292, doi: 10.1007/978-1-0716-2257-5_16, PMID: 35524123

Miro-Pina C, Charmant O, Kawaguchi T, Holoch D, Michaud A, Cohen I, Humbert A, Jaszczyszyn Y, Chevreux G, Del Maestro L, Ait-Si-Ali S, Arnaiz O, Margueron R, Duharcourt S (2022) Paramecium Polycomb repressive complex 2 physically interacts with the small RNA-binding PIWI protein to repress transposable elements. Dev Cell. 57: 1037-1052 e1038, doi: 10.1016/j.devcel.2022.03.014, PMID: 35429435

Liu R, Zhang W, Gou P, Berthelet J, Nian Q, Chevreux G, Legros V, Moroy G, Bui LC, Wang L, Dupret JM, Deshayes F, Rodrigues Lima F (2022) Cisplatin causes covalent inhibition of protein-tyrosine phosphatase 1B (PTP1B) through reaction with its active site cysteine: Molecular, cellular and in vivo mice studies. Biomed Pharmacother. 153: 113372, doi: 10.1016/j.biopha.2022.113372, PMID: 35809481

Liu D, Marie JC, Pelletier AL, Song Z, Ben-Khemis M, Boudiaf K, Pintard C, Leger T, Terrier S, Chevreux G, El-Benna J, Dang PM (2022) Protein Kinase CK2 Acts as a Molecular Brake to Control NADPH Oxidase 1 Activation and Colon Inflammation. Cell Mol Gastroenterol Hepatol. 13: 1073-1093, doi: 10.1016/j.jcmgh.2022.01.003, PMID: 35031518

2021

Senecaut N, Alves G, Weisser H, Lignieres L, Terrier S, Yang-Crosson L, Poulain P, Lelandais G, Yu YK, Camadro JM (2021) Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy. J Proteome Res. 20: 1476-1487, doi: 10.1021/acs.jproteome.0c00478, PMID: 33573382

Ribeiro-Parenti L, Jarry AC, Cavin JB, Willemetz A, Le Beyec J, Sannier A, Benadda S, Pelletier AL, Hourseau M, Leger T, Morlet B, Couvelard A, Anini Y, Msika S, Marmuse JP, Ledoux S, Le Gall M, Bado A (2021) Bariatric surgery induces a new gastric mucosa phenotype with increased functional glucagon-like peptide-1 expressing cells. Nat Commun. 12: 110, doi: 10.1038/s41467-020-20301-1, PMID: 33397977

Lemonnier T, Daldello EM, Poulhe R, Le T, Miot M, Lignieres L, Jessus C, Dupre A (2021) The M-phase regulatory phosphatase PP2A-B55delta opposes protein kinase A on Arpp19 to initiate meiotic division. Nat Commun. 12: 1837, doi: 10.1038/s41467-021-22124-0, PMID: 33758202

Lautier O, Penzo A, Rouviere JO, Chevreux G, Collet L, Loiodice I, Taddei A, Devaux F, Collart MA, Palancade B (2021) Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis. Mol Cell. 81: 2417-2427 e2415, doi: 10.1016/j.molcel.2021.03.030, PMID: 33838103

Gallo G, Caignard G, Badonnel K, Chevreux G, Terrier S, Szemiel A, Roman-Sosa G, Binder F, Gu Q, Da Silva Filipe A, Ulrich RG, Kohl A, Vitour D, Tordo N, Ermonval M (2021) Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling. Viruses. 13: doi: 10.3390/v13010140, PMID: 33478127

Darrigrand R, Pierson A, Rouillon M, Renko D, Boulpicante M, Bouyssie D, Mouton-Barbosa E, Marcoux J, Garcia C, Ghosh M, Alami M, Apcher S (2021) Isoginkgetin derivative IP2 enhances the adaptive immune response against tumor antigens. Commun Biol. 4: 269, doi: 10.1038/s42003-021-01801-2, PMID: 33649389

Bertini EV, Torres MA, Leger T, Garcia C, Hong KW, Chong TM, Castellanos de Figueroa LI, Chan KG, Dessaux Y, Camadro JM, Nieto-Penalver CG (2021) Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii. Genomics. 113: 4352-4360, doi: 10.1016/j.ygeno.2021.11.017, PMID: 34793950

Balint B, Hergalant S, Camadro JM, Blaise S, Vanalderwiert L, Lignieres L, Gueant-Rodriguez RM, Gueant JL (2021) Fetal Programming by Methyl Donor Deficiency Produces Pathological Remodeling of the Ascending Aorta. Arterioscler Thromb Vasc Biol. 41: 1928-1941, doi: 10.1161/ATVBAHA.120.315587, PMID: 33827257

2020

Morilla I, Leger T, Marah A, Pic I, Zaag H, Ogier-Denis E (2020) Singular manifolds of proteomic drivers to model the evolution of inflammatory bowel disease status. Sci Rep. 10: 19066, doi: 10.1038/s41598-020-76011-7, PMID: 33149233

Duval C, Macabiou C, Garcia C, Lesuisse E, Camadro JM, Auchere F (2020) The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans. Microbiologyopen. 9: e970, doi: 10.1002/mbo3.970, PMID: 31788966

2019

Scheiber IF, Pilatova J, Malych R, Kotabova E, Krijt M, Vyoral D, Mach J, Leger T, Camadro JM, Prasil O, Lesuisse E, Sutak R (2019) Copper and iron metabolism in Ostreococcus tauri – the role of phytotransferrin, plastocyanin and a chloroplast copper-transporting ATPase. Metallomics. 11: 1657-1666, doi: 10.1039/c9mt00078j, PMID: 31380866

Sanchez David RY, Combredet C, Najburg V, Millot GA, Beauclair G, Schwikowski B, Leger T, Camadro JM, Jacob Y, Bellalou J, Jouvenet N, Tangy F, Komarova AV (2019) LGP2 binds to PACT to regulate RIG-I- and MDA5-mediated antiviral responses. Sci Signal. 12: doi: 10.1126/scisignal.aar3993, PMID: 31575732

Renaudin F, Sarda S, Campillo-Gimenez L, Severac C, Leger T, Charvillat C, Rey C, Liote F, Camadro JM, Ea HK, Combes C (2019) Adsorption of Proteins on m-CPPD and Urate Crystals Inhibits Crystal-induced Cell Responses: Study on Albumin-crystal Interaction. J Funct Biomater. 10: doi: 10.3390/jfb10020018, PMID: 31027151

Nian Q, Berthelet J, Zhang W, Bui LC, Liu R, Xu X, Duval R, Ganesan S, Leger T, Chomienne C, Busi F, Guidez F, Dupret JM, Rodrigues Lima F (2019) T-Cell Protein Tyrosine Phosphatase Is Irreversibly Inhibited by Etoposide-Quinone, a Reactive Metabolite of the Chemotherapy Drug Etoposide. Mol Pharmacol. 96: 297-306, doi: 10.1124/mol.119.116319, PMID: 31221825

Massarweh A, Bosco M, Chantret I, Leger T, Jamal L, Roper DI, Dowson CG, Busca P, Bouhss A, Gravier-Pelletier C, Moore SEH (2019) Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities. Molecules. 24: doi: 10.3390/molecules24112135, PMID: 31174247

Lelandais G, Denecker T, Garcia C, Danila N, Leger T, Camadro JM (2019) Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to assess data reproducibility. BMC Res Notes. 12: 470, doi: 10.1186/s13104-019-4505-8, PMID: 31370875

Le Faouder J, Gigante E, Leger T, Albuquerque M, Beaufrere A, Soubrane O, Dokmak S, Camadro JM, Cros J, Paradis V (2019) Proteomic Landscape of Cholangiocarcinomas Reveals Three Different Subgroups According to Their Localization and the Aspect of Non-Tumor Liver. Proteomics Clin Appl. 13: e1800128, doi: 10.1002/prca.201800128, PMID: 30520266

Kundlacz C, Pourcelot M, Fablet A, Amaral Da Silva Moraes R, Leger T, Morlet B, Viarouge C, Sailleau C, Turpaud M, Gorlier A, Breard E, Lecollinet S, van Rijn PA, Zientara S, Vitour D, Caignard G (2019) Novel Function of Bluetongue Virus NS3 Protein in Regulation of the MAPK/ERK Signaling Pathway. J Virol. 93: doi: 10.1128/JVI.00336-19, PMID: 31167915

Fernandez J, Machado AK, Lyonnais S, Chamontin C, Gartner K, Leger T, Henriquet C, Garcia C, Portilho DM, Pugniere M, Chaloin L, Muriaux D, Yamauchi Y, Blaise M, Nisole S, Arhel NJ (2019) Transportin-1 binds to the HIV-1 capsid via a nuclear localization signal and triggers uncoating. Nat Microbiol. 4: 1840-1850, doi: 10.1038/s41564-019-0575-6, PMID: 31611641

Elzaiat M, Herman L, Legois B, Leger T, Todeschini AL, Veitia RA (2019) High-throughput Exploration of the Network Dependent on AKT1 in Mouse Ovarian Granulosa Cells. Mol Cell Proteomics. 18: 1307-1319, doi: 10.1074/mcp.RA119.0014613, PMID: 30992313

El Banna N, Hatem E, Heneman-Masurel A, Leger T, Baille D, Vernis L, Garcia C, Martineau S, Dupuy C, Vagner S, Camadro JM, Huang ME (2019) Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death. Redox Biol. 26: 101290, doi: 10.1016/j.redox.2019.101290, PMID: 31412312

Bouillier C, Cosentino G, Leger T, Rincheval V, Richard CA, Desquesnes A, Sitterlin D, Blouquit-Laye S, Eleouet JF, Gault E, Rameix-Welti MA (2019) The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription. Sci Rep. 9: 15258, doi: 10.1038/s41598-019-51746-0, PMID: 31649314

Bonnet J, Garcia C, Leger T, Couquet MP, Vignoles P, Vatunga G, Ndung’u J, Boudot C, Bisser S, Courtioux B (2019) Proteome characterization in various biological fluids of Trypanosoma brucei gambiense-infected subjects. J Proteomics. 196: 150-161, doi: 10.1016/j.jprot.2018.11.005, PMID: 30414516

2018

Telot L, Rousseau E, Lesuisse E, Garcia C, Morlet B, Leger T, Camadro JM, Serre V (2018) Quantitative proteomics in Friedreich’s ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis. Biochim Biophys Acta Mol Basis Dis. 1864: 997-1009, doi: 10.1016/j.bbadis.2018.01.010, PMID: 29329987

Obino D, Fetler L, Soza A, Malbec O, Saez JJ, Labarca M, Oyanadel C, Del Valle Batalla F, Goles N, Chikina A, Lankar D, Segovia-Miranda F, Garcia C, Leger T, Gonzalez A, Espeli M, Lennon-Dumenil AM, Yuseff MI (2018) Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse. Cell Rep. 25: 3110-3122 e3116, doi: 10.1016/j.celrep.2018.11.052, PMID: 30540943

Denard J, Rouillon J, Leger T, Garcia C, Lambert MP, Griffith G, Jenny C, Camadro JM, Garcia L, Svinartchouk F (2018) AAV-8 and AAV-9 Vectors Cooperate with Serum Proteins Differently Than AAV-1 and AAV-6. Mol Ther Methods Clin Dev. 10: 291-302, doi: 10.1016/j.omtm.2018.08.001, PMID: 30155509

2017

Richarme G, Liu C, Mihoub M, Abdallah J, Leger T, Joly N, Liebart JC, Jurkunas UV, Nadal M, Bouloc P, Dairou J, Lamouri A (2017) Guanine glycation repair by DJ-1/Park7 and its bacterial homologs. Science. 357: 208-211, doi: 10.1126/science.aag1095, PMID: 28596309

Planchon M, Leger T, Spalla O, Huber G, Ferrari R (2017) Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli. PLoS One. 12: e0178437, doi: 10.1371/journal.pone.0178437, PMID: 28570583

Leger T, Garcia C, Collomb L, Camadro JM (2017) A Simple Light Isotope Metabolic Labeling (SLIM-labeling) Strategy: A Powerful Tool to Address the Dynamics of Proteome Variations In Vivo. Mol Cell Proteomics. 16: 2017-2031, doi: 10.1074/mcp.M117.066936, PMID: 28821603

Hovsepian J, Defenouillere Q, Albanese V, Vachova L, Garcia C, Palkova Z, Leon S (2017) Multilevel regulation of an alpha-arrestin by glucose depletion controls hexose transporter endocytosis. J Cell Biol. 216: 1811-1831, doi: 10.1083/jcb.201610094, PMID: 28468835

Gergondey R, Garcia C, Marchand CH, Lemaire SD, Camadro JM, Auchere F (2017) Modulation of the specific glutathionylation of mitochondrial proteins in the yeast Saccharomyces cerevisiae under basal and stress conditions. Biochem J. 474: 1175-1193, doi: 10.1042/BCJ20160927, PMID: 28167699

Boukpessi T, Hoac B, Coyac BR, Leger T, Garcia C, Wicart P, Whyte MP, Glorieux FH, Linglart A, Chaussain C, McKee MD (2017) Osteopontin and the dento-osseous pathobiology of X-linked hypophosphatemia. Bone. 95: 151-161, doi: 10.1016/j.bone.2016.11.019, PMID: 27884786

News

15/06/2023 :

The ProtéoSeine platform is organizing a seminar in conjunction with Protein Metrics. The aim of this seminar will be to present the work carried out by the platform, in particular on the analysis of intact proteins.

Protein Metrics will present its software solutions for protein, peptide and oligonucleotide analysis.

The seminar will take place on the morning of Thursday June 15, 2023, at the Institut Jacques Monod.

Would you like to take part?

You can view the program and register here :

https://proteinmetrics.com/paris-symposium-2023/

06/02/2023

Jean-Michel Camadro’s team, in collaboration with the ProtéoSeine platform, the Ladoux/Mège team and Dumont, have just published a new article:

Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling

Laurent Lignières, Nicolas Sénécaut, Tien Dang, Laura Bellutti, Marion Hamon, Samuel Terrier, Véronique Legros, Guillaume Chevreux, Gaëlle Lelandais, René-Marc Mège, Julien Dumont, and Jean-Michel Camadro, Journal of Proteome Research, 2023, 22, 3, 996–1002