ImagoSeine

Flow cytometry, Light microscopy, Electron microscopy, Image analysis

 

PRESENTATION 

 

ImagoSeine is the service and R&D (Research & Development) platform in imaging of the Institut Jacques Monod.

ImagoSeine is a member of the national infrastructure of imaging platforms France-BioImaging and the Euro-BioImaging network.

It offers and develops high-level services for the visualisation and analysis of the structure, dynamics, interactions and functions of biological samples.

To do this, the Institut Jacques Monod’s ImagoSeine platform brings together flow cytometry, electron microscopy and photonic microscopy resources in a single location.

  • Flow cytometry allows qualitative and quantitative multiparametric and multicolour analysis, as well as high-throughput cell sorting, based on the size, granularity and fluorescence intensity of suspended elements.
  • Electron microscopy allows the ultrastructural analysis of the cell and its components. It includes transmission microscopy and sample preparation at room temperature or at low temperature.
  • Photonic microscopy allows the visualisation and analysis of the structure and dynamic processes at the cellular (prokaryotic and eukaryotic) and tissue levels. It offers expertise and innovative equipment, allowing observations from the single molecule to the scale of an organism.

The Institut Jacques Monod’s ImagoSeine platform is open to the entire national and international scientific community, both public and private.

ImagoSeine is supported by the following organisations:

MISSIONS 

 

We are a team of engineers, coming from different disciplines (biology, physics, computer science), working on the interface of microscopy with biology.
We propose :

  • the orientation of users towards the most suitable equipment for their project
  • the assistance in the design of tools: molecular biology, protein labeling, choice of labels and fluorophores, analysis protocols…
  • the assistance in setting up the experiment: definition of controls, operating procedures, precautions to be taken with respect to potential artifacts
  • the possible supervision of the users for the realization of the experiments
  • the provision of functional and calibrated equipments
  • the training of users for an autonomous use of the equipments
  • the taking in charge of the acquisitions for the non autonomous users
  • the assistance to the treatment and analysis of the obtained data
  • assistance in the interpretation and presentation of the results.

 

Services

 

The ImagoSeine imaging platform offers 3 types of services in photonic microscopy, cytometry and electron microscopy:

1 – Independent use of equipments and reagents

The autonomous user is previously trained by the platform staff on certain equipment or for the performance of certain operations (see Training). He/she benefits from extended use time slots (see Access times) and adapted rates (autonomous rate). During the sessions reserved for autonomous use (see Reservation), the platform’s engineers can be called upon for specific questions, or in the event of technical problems (malfunctions or defects in the equipment), during the platform’s normal opening hours, depending on their availability. If necessary, the autonomous user can book a session with assistance (advice, in-depth training). The autonomous user is responsible for the quality and management of the results he/she has obtained. A “session follow-up book”, located next to each device, must be filled in at each session. Documents (user’s manual, tutorials, …) are made available to the users and can be consulted on site. During the service, the autonomous user is responsible for the equipment provided. He/she must follow the rules for starting up, using, closing and storing the equipment and reagents used.  The team leader agrees to pay the full cost of repairs in the event of damage due to improper use of equipment. Any independent user who has not used equipment for more than one year or since the update of equipment (hardware, software) will have to undergo additional training. The status of autonomous user is subject to the appreciation of the platform staff and can be reviewed without notice. An autonomous user cannot train another user under any circumstances.

2 – The assisted session

The assisted session is conducted in the presence of the platform’s staff. It is proposed within the framework of the implementation of known protocols in standard experimental conditions, decided with the user, and does not have an inventive character. The engineers of the platform involved guarantee the quality of the results, provided that the use is made according to their recommendations, under the conditions fixed in agreement with the user. The session with assistance is subject to prior reservation (see Reservation), depending on the availability of the platform’s equipment and engineers and only during the normal opening hours (see Access hours). It is subject to “assisted” pricing.

3 – The collaborative project

This is the case when the user’s project requires the support of one or more platform engineers for occasional or regular assistance or for the development of a technique or technology for the user’s applications, or of a data acquisition or processing protocol. The platform’s engineers involved in the project guarantee the quality of the results obtained and their restitution (samples, data). The stages of a project can be carried out in the presence or absence of the user, depending on what has been decided beforehand between the user and the engineers involved. In the second case they are not subject to a particular reservation. Project invoicing may be subject to special pricing, based on an estimate, depending on the equipment used for the project. Billing will be based on actual hours used. The rate applied will be the “assisted users” rate.

GENERAL COORDINATION OF IMAGOSEINE 

 

René-Marc Mège – +33 (0)157278067 – rene-marc.mege@ijm.fr

Jean-Marc Verbavatz – +33 (0)157278004 – jean-marc.verbavatz@ijm.fr

Flow cytometry: cytometrie@ijm.fr

Electron microscopy:  microscopie.electronique@ijm.fr

Photonic microscopy: microscopie.photonique@ijm.fr

Platform members ImagoSeine :

Nicolas VALENTIN Engineer, Flow Cytometry Manager
Rémi LE BORGNE Engineer, Head of Electron Microscopy
Catherine DURIEU Electron microscopy engineer
Xavier BAUDIN Engineer, Head of Photonic Microscopy
Nicolas MOISAN Photonic microscopy research engineer
Thomas RIOS Photonic microscopy engineer
Romain NAILLON

How to proceed

 

1) Contact us by email to make an appointment with our team.

Flow cytometry: cytometrie@ijm.fr

Electron microscopy: microscopie.electronique@ijm.fr

Light microscopy: microscopie.photonique@ijm.fr

2) During this meeting it is necessary that the person in charge of the project is present and that you provide us with the important information (purpose of the experiment, type of samples, markers, support…).

It will be decided whether your project requires collaboration, service or training on one of the devices.

3) Register on the platform’s booking site.

You must have a valid professional email address. Once your information has been validated by the platform, your identifier (first name.surname) will be activated to connect you.

4) Create your password to connect to the booking site on this page. Once connected, download the charter, print and sign the last page.

 

Use of equipment and services

Only after the signed charter has been received can the services take place. Make requests for equipment training and collaborative projects once you are logged on to the booking site with the “Request” tab.

Charter to download from the reservation site

Billing

The use of the platform’s equipment is subject to a fee (see our rates). The rates are calculated to cover the maintenance and updating of the platform’s equipment.

A detailed statement of the services provided will be sent to you each month. In return, you must send a purchase order to the Institute’s accounting department.

 

ImagoSeine Flow cytometry core facility user fees

 

ImagoSeine Light Microscopy User Fees

 

For time laps acquisitions, the price is divided by 2 between 8pm and 9am and during the weekend.

 

ImagoSeine Electronic Microscopy User Fees

PUBLICATIONS CO-WRITTEN BY IMAGOSEINE MEMBERS (2017-2023)

Huret, C., Ferrayé, L., David, A., Mohamed, M., Valentin, N., Charlotte, F., Savignac, M., Goodhardt, M., Guéry, J.-C., Rougeulle, C., & Morey, C. (2024). Altered X-chromosome inactivation predisposes to autoimmunity. Science Advances, 10(18), eadn6537. https://doi.org/10.1126/sciadv.adn6537
Villares, M., Lourenço, N., Ktorza, I., Berthelet, J., Panagiotou, A., Richard, A., Amo, A., Koziy, Y., Medjkane, S., Valente, S., Fioravanti, R., Pioche-Durieu, C., Lignière, L., Chevreux, G., Mai, A., & Weitzman, J. B. (2024). Theileria parasites sequester host eIF5A to escape elimination by host-mediated autophagy. Nature Communications, 15(1), 2235. https://doi.org/10.1038/s41467-024-45022-7
Szabová, J., Mravec, F., Mokhtari, M., Le Borgne, R., Kalina, M., & Berret, J.-F. (2023). N,N,N-Trimethyl chitosan as a permeation enhancer for inhalation drug delivery: Interaction with a model pulmonary surfactant. International Journal of Biological Macromolecules, 239, 124235. https://doi.org/10.1016/j.ijbiomac.2023.124235
Dhouib, A., Mezghrani, B., Finocchiaro, G., Le Borgne, R., Berthet, M., Daydé-Cazals, B., Graillot, A., Ju, X., & Berret, J.-F. (2023). Synthesis of Stable Cerium Oxide Nanoparticles Coated with Phosphonic Acid-Based Functional Polymers. Langmuir: The ACS Journal of Surfaces and Colloids. https://doi.org/10.1021/acs.langmuir.3c00576
Ferreras, S., Singh, N. P., Le Borgne, R., Bun, P., Binz, T., Parton, R. G., Verbavatz, J.-M., Vannier, C., & Galli, T. (2023). A synthetic organelle approach to probe SNARE-mediated membrane fusion in a bacterial host. The Journal of Biological Chemistry, 102974. https://doi.org/10.1016/j.jbc.2023.102974
Siegfried, H., Borgne, R. L., Durieu, C., Laplace, T. D. A., Verraes, A., Daunas, L., Verbavatz, J.-M., & Heuzé, M. L. (2023). The ER tether VAPA is required for proper cell motility and anchors ER-PM contact sites to focal adhesions. bioRxiv. https://doi.org/10.1101/2022.10.17.512434
Sonam, S., Balasubramaniam, L., Lin, S.-Z., Ivan, Y. M. Y., Jaumà, I. P., Jebane, C., Karnat, M., Toyama, Y., Marcq, P., Prost, J., Mège, R.-M., Rupprecht, J.-F., & Ladoux, B. (2023). Mechanical stress driven by rigidity sensing governs epithelial stability. Nature Physics, 19, 132–141. https://doi.org/10.1038/s41567-022-01826-2
Chevalier, L., Pinar, M., Le Borgne, R., Durieu, C., Peñalva, M. A., Boudaoud, A., & Minc, N. (2023). Cell wall dynamics stabilize tip growth in a filamentous fungus. PLoS Biology, 21(1), e3001981. https://doi.org/10.1371/journal.pbio.3001981
Gonçalves Antunes, M., Sanial, M., Contremoulins, V., Carvalho, S., Plessis, A., & Becam, I. (2022). High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothened. ELife, 11, e79843. https://doi.org/10.7554/eLife.79843
Zaaboub, R., Vimeux, L., Contremoulins, V., Cymbalista, F., Lévy, V., Donnadieu, E., Varin-Blank, N., Martin, A., & Dondi, E. (2022). Nurselike cells sequester B cells in disorganized lymph nodes in chronic lymphocytic leukemia via alternative production of CCL21. Blood Advances, 6(16), 4691–4704. https://doi.org/10.1182/bloodadvances.2021006169
Lachat, J., Pascault, A., Thibaut, D., Le Borgne, R., Verbavatz, J.-M., & Weiner, A. (2022). Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage. Nature Communications, 13(1), 3781. https://doi.org/10.1038/s41467-022-31237-z
Fernandes, P., Loubens, M., Le Borgne, R., Marinach, C., Ardin, B., Briquet, S., Vincensini, L., Hamada, S., Hoareau-Coudert, B., Verbavatz, J.-M., Weiner, A., & Silvie, O. (2022). The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts. PLoS Pathogens, 18(6), e1010643. https://doi.org/10.1371/journal.ppat.1010643
Gaudin, N., Martin Gil, P., Boumendjel, M., Ershov, D., Pioche-Durieu, C., Bouix, M., Delobelle, Q., Maniscalco, L., Phan, T. B. N., Heyer, V., Reina-San-Martin, B., & Azimzadeh, J. (2022). Evolutionary conservation of centriole rotational asymmetry in the human centrosome. ELife, 11, e72382. https://doi.org/10.7554/eLife.72382
Sonam, S., Balasubramaniam, L., Lin, S.-Z., Ivan, Y. M. Y., Jaumà, I. P., Jebane, C., Karnat, M., Toyama, Y., Marcq, P., Prost, J., Mège, R.-M., Rupprecht, J.-F., & Ladoux, B. (2022). Mechanical stress driven by rigidity sensing governs epithelial stability. bioRxiv. https://doi.org/10.1101/2022.03.10.483785
Deshayes, F., Fradet, M., Kaminski, S., Viguier, M., Frippiat, J.-P., & Ghislin, S. (2022). Link between the EZH2 noncanonical pathway and microtubule organization center polarization during early T lymphopoiesis. Scientific Reports, 12(1), 3655. https://doi.org/10.1038/s41598-022-07684-5
Xi, W., Saleh, J., Yamada, A., Tomba, C., Mercier, B., Janel, S., Dang, T., Soleilhac, M., Djemat, A., Wu, H., Romagnolo, B., Lafont, F., Mège, R.-M., Chen, Y., & Delacour, D. (2022). Modulation of designer biomimetic matrices for optimized differentiated intestinal epithelial cultures. Biomaterials, 282, 121380. https://doi.org/10.1016/j.biomaterials.2022.121380
Xie, J., Najafi, J., Le Borgne, R., Verbavatz, J.-M., Durieu, C., Sallé, J., & Minc, N. (2022). Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning. Proceedings of the National Academy of Sciences of the United States of America, 119(8), e2115593119. https://doi.org/10.1073/pnas.2115593119
Dussouchaud, A., Jacob, J., Secq, C., Verbavatz, J.-M., Moras, M., Larghero, J., Fader, C. M., Ostuni, M. A., & Lefevre, S. D. (2022). Transmission Electron Microscopy to Follow Ultrastructural Modifications of Erythroblasts Upon ex vivo Human Erythropoiesis. Frontiers in Physiology, 12, 791691. https://doi.org/10.3389/fphys.2021.791691
Nedara, K., Reinhardt, C., Lebraud, E., Arena, G., Gracia, C., Buard, V., Pioche-Durieu, C., Castelli, F., Colsch, B., Bénit, P., Rustin, P., Albaud, B., Gestraud, P., Baulande, S., Servant, N., Deutsch, E., Verbavatz, J.-M., Brenner, C., Milliat, F., & Modjtahedi, N. (2022). Relevance of the TRIAP1/p53 axis in colon cancer cell proliferation and adaptation to glutamine deprivation. Frontiers in Oncology, 12, 958155. https://doi.org/10.3389/fonc.2022.958155
Sonam, S., Vigouroux, C., Jégou, A., Romet-Lemonne, G., Le Clainche, C., Ladoux, B., & Mège, R. M. (2021). Direct measurement of near-nano-Newton forces developed by self-organizing actomyosin fibers bound α-catenin. Biology of the Cell, 113(11), 441–449. https://doi.org/10.1111/boc.202100014
Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mège, R.-M., Yeomans, J. M., & Ladoux, B. (2021). Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1156–1166. https://doi.org/10.1038/s41563-021-00919-2
d’Alessandro, J., Barbier-Chebbah, A., Cellerin, V., Benichou, O., Mège, R. M., Voituriez, R., & Ladoux, B. (2021). Cell migration guided by long-lived spatial memory. Nature Communications, 12(1), 4118. https://doi.org/10.1038/s41467-021-24249-8
Sellis, D., Guérin, F., Arnaiz, O., Pett, W., Lerat, E., Boggetto, N., Krenek, S., Berendonk, T., Couloux, A., Aury, J.-M., Labadie, K., Malinsky, S., Bhullar, S., Meyer, E., Sperling, L., Duret, L., & Duharcourt, S. (2021). Massive colonization of protein-coding exons by selfish genetic elements in Paramecium germline genomes. PLoS Biology, 19(7), e3001309. https://doi.org/10.1371/journal.pbio.3001309
Bernard, F., Jouette, J., Durieu, C., Le Borgne, R., Guichet, A., & Claret, S. (2021). GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool in Drosophila. Frontiers in Cell and Developmental Biology, 9, 719582. https://doi.org/10.3389/fcell.2021.719582
Gonzalez Curto, G., Der Vartanian, A., Frarma, Y. E.-M., Manceau, L., Baldi, L., Prisco, S., Elarouci, N., Causeret, F., Korenkov, D., Rigolet, M., Aurade, F., De Reynies, A., Contremoulins, V., Relaix, F., Faklaris, O., Briscoe, J., Gilardi-Hebenstreit, P., & Ribes, V. (2020). The PAX-FOXO1s trigger fast trans-differentiation of chick embryonic neural cells into alveolar rhabdomyosarcoma with tissue invasive properties limited by S phase entry inhibition. PLoS Genetics, 16(11), e1009164. https://doi.org/10.1371/journal.pgen.1009164
Velez-Aguilera, G., Nkombo Nkoula, S., Ossareh-Nazari, B., Link, J., Paouneskou, D., Van Hove, L., Joly, N., Tavernier, N., Verbavatz, J.-M., Jantsch, V., & Pintard, L. (2020). PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly. ELife, 9, e59510. https://doi.org/10.7554/eLife.59510
Déjardin, T., Carollo, P. S., Sipieter, F., Davidson, P. M., Seiler, C., Cuvelier, D., Cadot, B., Sykes, C., Gomes, E. R., & Borghi, N. (2020). Nesprins are mechanotransducers that discriminate epithelial-mesenchymal transition programs. The Journal of Cell Biology, 219(10), e201908036. https://doi.org/10.1083/jcb.201908036
Raich, N., Contremoulins, V., & Karess, R. E. (2020). Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes. Journal of Visualized Experiments: JoVE, 162. https://doi.org/10.3791/61061
Jain, S., Cachoux, V. M. L., Narayana, G. H. N. S., de Beco, S., D’Alessandro, J., Cellerin, V., Chen, T., Heuzé, M. L., Marcq, P., Mège, R.-M., Kabla, A. J., Lim, C. T., & Ladoux, B. (2020). The role of single cell mechanical behavior and polarity in driving collective cell migration. Nature Physics, 16(7), 802–809. https://doi.org/10.1038/s41567-020-0875-z
Botté, A., Lainé, J., Xicota, L., Heiligenstein, X., Fontaine, G., Kasri, A., Rivals, I., Goh, P., Faklaris, O., Cossec, J.-C., Morel, E., Rebillat, A.-S., Nizetic, D., Raposo, G., & Potier, M.-C. (2020). Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome. Acta Neuropathologica Communications, 8(1), 89. https://doi.org/10.1186/s40478-020-00956-z
Doss, B. L., Pan, M., Gupta, M., Grenci, G., Mège, R.-M., Lim, C. T., Sheetz, M. P., Voituriez, R., & Ladoux, B. (2020). Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress. Proceedings of the National Academy of Sciences of the United States of America, 117(23), 12817–12825. https://doi.org/10.1073/pnas.1917555117
Bidel, L. P. R., Meyer, S., Talhouët, A.-C., Baudin, X., Daniel, C., Cazals, G., & Streb, P. (2020). Epidermal UVA screening capacity measured in situ as an indicator of light acclimation state of leaves of a very plastic alpine plant Soldanella alpina L. Plant Physiology and Biochemistry: PPB, 151, 10–20. https://doi.org/10.1016/j.plaphy.2020.02.045
Debernardi, J., Pioche-Durieu, C., Cam, E. L., Wiels, J., & Robert, A. (2020). Verotoxin-1-Induced ER Stress Triggers Apoptotic or Survival Pathways in Burkitt Lymphoma Cells. Toxins, 12(5), 316. https://doi.org/10.3390/toxins12050316
Plan Sangnier, A., Van de Walle, A., Aufaure, R., Fradet, M., Motte, L., Guénin, E., Lalatonne, Y., & Wilhelm, C. (2020). Endosomal Confinement of Gold Nanospheres, Nanorods, and Nanoraspberries Governs Their Photothermal Identity and Is Beneficial for Cancer Cell Therapy. Advanced Biosystems, 4(4), e1900284. https://doi.org/10.1002/adbi.201900284
Talhouët, A.-C., Meyer, S., Baudin, X., & Streb, P. (2020). Dynamic acclimation to sunlight in an alpine plant, Soldanella alpina L. Physiologia Plantarum, 168(3), 563–575. https://doi.org/10.1111/ppl.12982
Dilsizoglu Senol, A., Tagliafierro, L., Gorisse-Hussonnois, L., Rebeillard, F., Huguet, L., Geny, D., Contremoulins, V., Corlier, F., Potier, M.-C., Chasseigneaux, S., Darmon, M., & Allinquant, B. (2019). Protein interacting with Amyloid Precursor Protein tail-1 (PAT1) is involved in early endocytosis. Cellular and Molecular Life Sciences: CMLS, 76(24), 4995–5009. https://doi.org/10.1007/s00018-019-03157-7
Sanchez-Guzman, D., Le Guen, P., Villeret, B., Sola, N., Le Borgne, R., Guyard, A., Kemmel, A., Crestani, B., Sallenave, J.-M., & Garcia-Verdugo, I. (2019). Silver nanoparticle-adjuvanted vaccine protects against lethal influenza infection through inducing BALT and IgA-mediated mucosal immunity. Biomaterials, 217, 119308. https://doi.org/10.1016/j.biomaterials.2019.119308
Plan Sangnier, A., Van de Walle, A. B., Curcio, A., Le Borgne, R., Motte, L., Lalatonne, Y., & Wilhelm, C. (2019). Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells. Nanoscale, 11(35), 16488–16498. https://doi.org/10.1039/c9nr05624f
Heuzé, M. L., Sankara Narayana, G. H. N., D’Alessandro, J., Cellerin, V., Dang, T., Williams, D. S., Van Hest, J. C., Marcq, P., Mège, R.-M., & Ladoux, B. (2019). Myosin II isoforms play distinct roles in adherens junction biogenesis. ELife, 8, e46599. https://doi.org/10.7554/eLife.46599
Peyret, G., Mueller, R., d’Alessandro, J., Begnaud, S., Marcq, P., Mège, R.-M., Yeomans, J. M., Doostmohammadi, A., & Ladoux, B. (2019). Sustained Oscillations of Epithelial Cell Sheets. Biophysical Journal, 117(3), 464–478. https://doi.org/10.1016/j.bpj.2019.06.013
Nguyen, T., Duchesne, L., Sankara Narayana, G. H. N., Boggetto, N., Fernig, D. D., Uttamrao Murade, C., Ladoux, B., & Mège, R.-M. (2019). Enhanced cell-cell contact stability and decreased N-cadherin-mediated migration upon fibroblast growth factor receptor-N-cadherin cross talk. Oncogene, 38(35), 6283–6300. https://doi.org/10.1038/s41388-019-0875-6
Duval, N., Vaslin, C., Barata, T. C., Frarma, Y., Contremoulins, V., Baudin, X., Nedelec, S., & Ribes, V. C. (2019). BMP4 patterns Smad activity and generates stereotyped cell fate organization in spinal organoids. Development (Cambridge, England), 146(14), dev175430. https://doi.org/10.1242/dev.175430
Chevalier, N. R., Dacher, N., Jacques, C., Langlois, L., Guedj, C., & Faklaris, O. (2019). Embryogenesis of the peristaltic reflex. The Journal of Physiology, 597(10), 2785–2801. https://doi.org/10.1113/JP277746
Collot, M., Ashokkumar, P., Anton, H., Boutant, E., Faklaris, O., Galli, T., Mély, Y., Danglot, L., & Klymchenko, A. S. (2019). MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Cell Chemical Biology, 26(4), 600-614.e7. https://doi.org/10.1016/j.chembiol.2019.01.009
Gauquelin, E., Tlili, S., Gay, C., Peyret, G., Mège, R.-M., Fardin, M. A., & Ladoux, B. (2019). Influence of proliferation on the motions of epithelial monolayers invading adherent strips. Soft Matter, 15(13), 2798–2810. https://doi.org/10.1039/c9sm00105k
Gupta, M., Doss, B. L., Kocgozlu, L., Pan, M., Mège, R.-M., Callan-Jones, A., Voituriez, R., & Ladoux, B. (2019). Cell shape and substrate stiffness drive actin-based cell polarity. Physical Review. E, 99(1–1), 012412. https://doi.org/10.1103/PhysRevE.99.012412
Walch, L., Pellier, E., Leng, W., Lakisic, G., Gautreau, A., Contremoulins, V., Verbavatz, J.-M., & Jackson, C. L. (2018). GBF1 and Arf1 interact with Miro and regulate mitochondrial positioning within cells. Scientific Reports, 8(1), 17121. https://doi.org/10.1038/s41598-018-35190-0
Fernandes, J., Hamidi, F., Leborgne, R., Beau, R., Castier, Y., Mordant, P., Boukkerou, A., Latgé, J. P., & Pretolani, M. (2018). Penetration of the Human Pulmonary Epithelium by Aspergillus fumigatus Hyphae. The Journal of Infectious Diseases, 218(8), 1306–1313. https://doi.org/10.1093/infdis/jiy298
Daste, F., Sauvanet, C., Bavdek, A., Baye, J., Pierre, F., Le Borgne, R., David, C., Rojo, M., Fuchs, P., & Tareste, D. (2018). The heptad repeat domain 1 of Mitofusin has membrane destabilization function in mitochondrial fusion. EMBO Reports, 19(6), e43637. https://doi.org/10.15252/embr.201643637
Penrad-Mobayed, M., Perrin, C., L’Hôte, D., Contremoulins, V., Lepesant, J.-A., Boizet-Bonhoure, B., Poulat, F., Baudin, X., & Veitia, R. A. (2018). A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte. Scientific Reports, 8(1), 7191. https://doi.org/10.1038/s41598-018-25356-1

 

PUBLICATIONS WITH THANKS FROM THE IMAGOSEINE PLATFORM (2017-2023)

Leclercq, B., Weiner, A., Zola, M., Mejlacowicz, D., Lassiaz, P., Jonet, L., Gélizé, E., Perrot, J., Viengchareun, S., Zhao, M., & Behar-Cohen, F. (2023). The choroidal nervous system: a link between mineralocorticoid receptor and pachychoroid. Acta Neuropathologica. https://doi.org/10.1007/s00401-023-02628-3
Augustin, S., Lam, M., Lavalette, S., Verschueren, A., Blond, F., Forster, V., Przegralek, L., He, Z., Lewandowski, D., Bemelmans, A.-P., Picaud, S., Sahel, J.-A., Mathis, T., Paques, M., Thuret, G., Guillonneau, X., Delarasse, C., & Sennlaub, F. (2023). Melanophages give rise to hyperreflective foci in AMD, a disease-progression marker. Journal of Neuroinflammation, 20(1), 28. https://doi.org/10.1186/s12974-023-02699-9
Siegfried, H., Borgne, R. L., Durieu, C., Laplace, T. D. A., Verraes, A., Daunas, L., Verbavatz, J.-M., & Heuzé, M. L. (2023). The ER tether VAPA is required for proper cell motility and anchors ER-PM contact sites to focal adhesions. bioRxiv. https://doi.org/10.1101/2022.10.17.512434
Coly, P.-M., Chatterjee, S., Mezine, F., Jekmek, C. E., Devue, C., Nipoti, T., Corona, M. L., Dingli, F., Loew, D., Niel, G. van, & Boulanger, C. M. (2023). Atheroprone shear stress stimulates noxious endothelial extracellular vesicle uptake by MCAM and PECAM-1 cell adhesion molecules. bioRxiv. https://doi.org/10.1101/2022.12.31.522373
Berret, J.-F., & Graillot, A. (2022). Versatile Coating Platform for Metal Oxide Nanoparticles: Applications to Materials and Biological Science. Langmuir, 38(18), 5323–5338. https://doi.org/10.1021/acs.langmuir.2c00338
Velez-Aguilera, G., Ossareh-Nazari, B., Van Hove, L., Joly, N., & Pintard, L. (2022). Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote. ELife, 11, e75382. https://doi.org/10.7554/eLife.75382
Loh, M., Dauvet, D., Sanchez-Garrido, F., Sadaouli, K., Bernard, F., & Guichet, A. (2022). Kinesin-1 promotes centrosome clustering and nuclear migration in the Drosophila oocyte [Preprint]. Developmental Biology. https://doi.org/10.1101/2022.02.16.480671
Brun, S., Kuo, H.-C., Jeffree, C. E., Thomson, D. D., & Read, N. (2021). Courtship Ritual of Male and Female Nuclei during Fertilization in Neurospora crassa. Microbiology Spectrum, 9(2), e0033521. https://doi.org/10.1128/Spectrum.00335-21
Tovey, C. A., Tsuji, C., Egerton, A., Bernard, F., Guichet, A., de la Roche, M., & Conduit, P. T. (2021). Autoinhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects. The Journal of Cell Biology, 220(8), e202010020. https://doi.org/10.1083/jcb.202010020
Gemin, O., Serna, P., Zamith, J., Assendorp, N., Fossati, M., Rostaing, P., Triller, A., & Charrier, C. (2021). Unique properties of dually innervated dendritic spines in pyramidal neurons of the somatosensory cortex uncovered by 3D correlative light and electron microscopy. PLoS Biology, 19(8), e3001375. https://doi.org/10.1371/journal.pbio.3001375
Chevalier, N. R., Ammouche, Y., Gomis, A., Langlois, L., Guilbert, T., Bourdoncle, P., & Dufour, S. (2021). A neural crest cell isotropic-to-nematic phase transition in the developing mammalian gut. Communications Biology, 4(1), 770. https://doi.org/10.1038/s42003-021-02333-5
Gonzalez-Estevez, A., Verrico, A., Orniacki, C., Reina-San-Martin, B., & Doye, V. (2021). Integrity of the short arm of the nuclear pore Y-complex is required for mouse embryonic stem cell growth and differentiation. Journal of Cell Science, 134(10), jcs258340. https://doi.org/10.1242/jcs.258340
Giménez-Andrés, M., Emeršič, T., Antoine-Bally, S., D’Ambrosio, J. M., Antonny, B., Derganc, J., & Čopič, A. (2021). Exceptional stability of a perilipin on lipid droplets depends on its polar residues, suggesting multimeric assembly. ELife, 10, e61401. https://doi.org/10.7554/eLife.61401
Gaston, C., De Beco, S., Doss, B., Pan, M., Gauquelin, E., D’Alessandro, J., Lim, C. T., Ladoux, B., & Delacour, D. (2021). EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nature Communications, 12(1), 2226. https://doi.org/10.1038/s41467-021-22482-9
Canever, H., Carollo, P. S., Fleurisson, R., Girard, P. P., & Borghi, N. (2021). Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition. Methods in Molecular Biology (Clifton, N.J.), 2179, 289–299. https://doi.org/10.1007/978-1-0716-0779-4_22
Chevalier, N. R., Agbesi, R. J. A., Ammouche, Y., & Dufour, S. (2021). How Smooth Muscle Contractions Shape the Developing Enteric Nervous System. Frontiers in Cell and Developmental Biology, 9, 678975. https://doi.org/10.3389/fcell.2021.678975
Brossas, C., Valton, A.-L., Venev, S. V., Chilaka, S., Counillon, A., Laurent, M., Goncalves, C., Duriez, B., Picard, F., Dekker, J., & Prioleau, M.-N. (2020). Clustering of strong replicators associated with active promoters is sufficient to establish an early-replicating domain. The EMBO Journal, 39(21), e99520. https://doi.org/10.15252/embj.201899520
Mukherjee, R. N., Sallé, J., Dmitrieff, S., Nelson, K. M., Oakey, J., Minc, N., & Levy, D. L. (2020). The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos. Developmental Cell, 54(3), 395-409.e7. https://doi.org/10.1016/j.devcel.2020.05.003
Bruzzone, L., Argüelles, C., Sanial, M., Miled, S., Alvisi, G., Gonçalves-Antunes, M., Qasrawi, F., Holmgren, R. A., Smibert, C. A., Lipshitz, H. D., Boccaccio, G. L., Plessis, A., & Bécam, I. (2020). Regulation of the RNA-binding protein Smaug by the GPCR Smoothened via the kinase Fused. EMBO Reports, 21(7), e48425. https://doi.org/10.15252/embr.201948425
de Vanssay, A., Touzeau, A., Arnaiz, O., Frapporti, A., Phipps, J., & Duharcourt, S. (2020). The Paramecium histone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements. PLoS Genetics, 16(7), e1008949. https://doi.org/10.1371/journal.pgen.1008949
D’Ambrosio, J. M., Albanèse, V., Lipp, N.-F., Fleuriot, L., Debayle, D., Drin, G., & Čopič, A. (2020). Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast. Journal of Cell Science, 133(11), jcs243733. https://doi.org/10.1242/jcs.243733
Joly, N., Beaumale, E., Van Hove, L., Martino, L., & Pintard, L. (2020). Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development. The Journal of Cell Biology, 219(6), e201912037. https://doi.org/10.1083/jcb.201912037
Coppin, L., Jannin, A., Ait Yahya, E., Thuillier, C., Villenet, C., Tardivel, M., Bongiovanni, A., Gaston, C., de Beco, S., Barois, N., van Seuningen, I., Durand, E., Bonnefond, A., Vienne, J.-C., Vamecq, J., Figeac, M., Vincent, A., Delacour, D., Porchet, N., & Pigny, P. (2020). Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface. Cell Death & Disease, 11(5), 360. https://doi.org/10.1038/s41419-020-2556-3
Borne, F., Kovalev, A., Gorb, S., & Courtier-Orgogozo, V. (2020). The glue produced by Drosophila melanogaster for pupa adhesion is universal. The Journal of Experimental Biology, 223(Pt 8), jeb220608. https://doi.org/10.1242/jeb.220608
Thai, L.-P.-A., Mousseau, F., Oikonomou, E., Radiom, M., & Berret, J.-F. (2020). Effect of Nanoparticles on the Bulk Shear Viscosity of a Lung Surfactant Fluid. ACS Nano, 14(1), 466–475. https://doi.org/10.1021/acsnano.9b06293
Pizon, V., Gaudin, N., Poteau, M., Cifuentes-Diaz, C., Demdou, R., Heyer, V., Reina San Martin, B., & Azimzadeh, J. (2020). hVFL3/CCDC61 is a component of mother centriole subdistal appendages required for centrosome cohesion and positioning. Biology of the Cell, 112(1), 22–37. https://doi.org/10.1111/boc.201900038
Casanova, M., Moscatelli, M., Chauvière, L. É., Huret, C., Samson, J., Liyakat Ali, T. M., Rosspopoff, O., & Rougeulle, C. (2019). A primate-specific retroviral enhancer wires the XACT lncRNA into the core pluripotency network in humans. Nature Communications, 10(1), 5652. https://doi.org/10.1038/s41467-019-13551-1
Basquin, C., Ershov, D., Gaudin, N., Vu, H. T.-K., Louis, B., Papon, J.-F., Orfila, A.-M., Mansour, S., Rink, J. C., & Azimzadeh, J. (2019). Emergence of a Bilaterally Symmetric Pattern from Chiral Components in the Planarian Epidermis. Developmental Cell, 51(4), 516-525.e5. https://doi.org/10.1016/j.devcel.2019.10.021
Mercier, A., Clairet, C., Debuchy, R., Morais, D., Silar, P., & Brun, S. (2019). The mitochondrial translocase of the inner membrane PaTim54 is involved in defense response and longevity in Podospora anserina. Fungal Genetics and Biology: FG & B, 132, 103257. https://doi.org/10.1016/j.fgb.2019.103257
Antunes, J. C., Benarroch, L., Moraes, F. C., Juenet, M., Gross, M.-S., Aubart, M., Boileau, C., Caligiuri, G., Nicoletti, A., Ollivier, V., Chaubet, F., Letourneur, D., & Chauvierre, C. (2019). Core-Shell Polymer-Based Nanoparticles Deliver miR-155-5p to Endothelial Cells. Molecular Therapy. Nucleic Acids, 17, 210–222. https://doi.org/10.1016/j.omtn.2019.05.016
Lipp, N.-F., Gautier, R., Magdeleine, M., Renard, M., Albanèse, V., Čopič, A., & Drin, G. (2019). An electrostatic switching mechanism to control the lipid transfer activity of Osh6p. Nature Communications, 10(1), 3926. https://doi.org/10.1038/s41467-019-11780-y
Demoor, A., Silar, P., & Brun, S. (2019). Appressorium: The Breakthrough in Dikarya. Journal of Fungi (Basel, Switzerland), 5(3), E72. https://doi.org/10.3390/jof5030072
Davì, V., Chevalier, L., Guo, H., Tanimoto, H., Barrett, K., Couturier, E., Boudaoud, A., & Minc, N. (2019). Systematic mapping of cell wall mechanics in the regulation of cell morphogenesis. Proceedings of the National Academy of Sciences of the United States of America, 116(28), 13833–13838. https://doi.org/10.1073/pnas.1820455116
Mousseau, F., Berret, J.-F., & Oikonomou, E. K. (2019). Design and Applications of a Fluorescent Labeling Technique for Lipid and Surfactant Preformed Vesicles. ACS Omega, 4(6), 10485–10493. https://doi.org/10.1021/acsomega.9b01094
Frapporti, A., Miró Pina, C., Arnaiz, O., Holoch, D., Kawaguchi, T., Humbert, A., Eleftheriou, E., Lombard, B., Loew, D., Sperling, L., Guitot, K., Margueron, R., & Duharcourt, S. (2019). The Polycomb protein Ezl1 mediates H3K9 and H3K27 methylation to repress transposable elements in Paramecium. Nature Communications, 10(1), 2710. https://doi.org/10.1038/s41467-019-10648-5
Duriez, B., Chilaka, S., Bercher, J.-F., Hercul, E., & Prioleau, M.-N. (2019). Replication dynamics of individual loci in single living cells reveal changes in the degree of replication stochasticity through S phase. Nucleic Acids Research, 47(10), 5155–5169. https://doi.org/10.1093/nar/gkz220
Thai, L. P. A., Mousseau, F., Oikonomou, E. K., & Berret, J.-F. (2019). On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy. Colloids and Surfaces. B, Biointerfaces, 178, 337–345. https://doi.org/10.1016/j.colsurfb.2019.03.020
Chen, T., Callan-Jones, A., Fedorov, E., Ravasio, A., Brugués, A., Ong, H. T., Toyama, Y., Low, B. C., Trepat, X., Shemesh, T., Voituriez, R., & Ladoux, B. (2019). Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nature Physics, 15, 393–402. https://doi.org/10.1038/s41567-018-0383-6
Cianciolo Cosentino, C., Berto, A., Pelletier, S., Hari, M., Loffing, J., Neuhauss, S. C. F., & Doye, V. (2019). Moderate Nucleoporin 133 deficiency leads to glomerular damage in zebrafish. Scientific Reports, 9(1), 4750. https://doi.org/10.1038/s41598-019-41202-4
Van de Walle, A., Plan Sangnier, A., Abou-Hassan, A., Curcio, A., Hémadi, M., Menguy, N., Lalatonne, Y., Luciani, N., & Wilhelm, C. (2019). Biosynthesis of magnetic nanoparticles from nano-degradation products revealed in human stem cells. Proceedings of the National Academy of Sciences of the United States of America, 116(10), 4044–4053. https://doi.org/10.1073/pnas.1816792116
Sallé, J., Xie, J., Ershov, D., Lacassin, M., Dmitrieff, S., & Minc, N. (2019). Asymmetric division through a reduction of microtubule centering forces. The Journal of Cell Biology, 218(3), 771–782. https://doi.org/10.1083/jcb.201807102
Jouette, J., Guichet, A., & Claret, S. B. (2019). Dynein-mediated transport and membrane trafficking control PAR3 polarised distribution. ELife, 8, e40212. https://doi.org/10.7554/eLife.40212
Planques, A., Malem, J., Parapar, J., Vervoort, M., & Gazave, E. (2019). Morphological, cellular and molecular characterization of posterior regeneration in the marine annelid Platynereis dumerilii. Developmental Biology, 445(2), 189–210. https://doi.org/10.1016/j.ydbio.2018.11.004
Baldim, V., Bia, N., Graillot, A., Loubat, C., & Berret, J. (2019). Monophosphonic versus Multiphosphonic Acid Based PEGylated Polymers for Functionalization and Stabilization of Metal (Ce, Fe, Ti, Al) Oxide Nanoparticles in Biological Media. Advanced Materials Interfaces, 6(7), 1801814. https://doi.org/10.1002/admi.201801814
D’Ambrosio, J. M., Albanèse, V., & Čopič, A. (2019). Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time. Methods in Molecular Biology (Clifton, N.J.), 1949, 35–46. https://doi.org/10.1007/978-1-4939-9136-5_4
Piquet, S., Le Parc, F., Bai, S.-K., Chevallier, O., Adam, S., & Polo, S. E. (2018). The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage. Molecular Cell, 72(5), 888-901.e7. https://doi.org/10.1016/j.molcel.2018.09.010
De Pascalis, C., Pérez-González, C., Seetharaman, S., Boëda, B., Vianay, B., Burute, M., Leduc, C., Borghi, N., Trepat, X., & Etienne-Manneville, S. (2018). Intermediate filaments control collective migration by restricting traction forces and sustaining cell-cell contacts. The Journal of Cell Biology, 217(9), 3031–3044. https://doi.org/10.1083/jcb.201801162
Plan Sangnier, A., Preveral, S., Curcio, A., K A Silva, A., Lefèvre, C. T., Pignol, D., Lalatonne, Y., & Wilhelm, C. (2018). Targeted thermal therapy with genetically engineered magnetite magnetosomes@RGD: Photothermia is far more efficient than magnetic hyperthermia. Journal of Controlled Release: Official Journal of the Controlled Release Society, 279, 271–281. https://doi.org/10.1016/j.jconrel.2018.04.036
Souquet, B., Freed, E., Berto, A., Andric, V., Audugé, N., Reina-San-Martin, B., Lacy, E., & Doye, V. (2018). Nup133 Is Required for Proper Nuclear Pore Basket Assembly and Dynamics in Embryonic Stem Cells. Cell Reports, 23(8), 2443–2454. https://doi.org/10.1016/j.celrep.2018.04.070
Lacroix, B., Letort, G., Pitayu, L., Sallé, J., Stefanutti, M., Maton, G., Ladouceur, A.-M., Canman, J. C., Maddox, P. S., Maddox, A. S., Minc, N., Nédélec, F., & Dumont, J. (2018). Microtubule Dynamics Scale with Cell Size to Set Spindle Length and Assembly Timing. Developmental Cell, 45(4), 496-511.e6. https://doi.org/10.1016/j.devcel.2018.04.022
October 2022: New LSM980 Airyscan2 scanning confocal and two-photon module available as part RH26B.
Latest generation Zeiss confocal, equipped for live experiments, allowing simultaneous spectral separation and FRAP experiments. The Airyscan 4Y module allows a 50% increase in resolution compared to a conventional confocal and an increase in acquisition speed. The two-photon microscopy module allows for more in-depth observation of samples.
September 2022 : New ELYRA7 Super-Resolution microscopy system in RH22B room.
Microscope allowing PALM and dSTORM experiments for localization accuracy down to 20nm.  Lattice SIM experiments allowing a 3D resolution increase of two times the theoretical limit and compatible with the living. TIRF experiments to observe events at the interface between the cell membrane and its substrate.
June 2022: New FLIM and FCS module on the confocal LSM980 in room RH20B1.
The FLIM technique allows studying the fluorescence lifetime in the sample, which gives information on its biological state as well as the interaction between proteins by the FLIM-FRET technique. FCS techniques allow the study of the mobility of fluorescent molecules.
September 2021: Upgrade of the CSU X1 FRAP spinning disk and UV Photoablation in RH20B9.
The spinning disk X1 FRAP has a new laser bench (405nm, 445nm, 488nm, 561nm, 642nm) as well as the possibility to do FRAP with the 5 lasers and photoablation by pulsed UV.