Mechanotransduction: from Cell Surface to Nucleus

How our cells respond to their mechanical environment?
We propose an approach using quantitative fluorescence microscopy and genetic engineering for responses from the molecule to the tissue.
Group Leader: Nicolas Borghi
Directeur de Recherche CNRS
+33 (0)157278041
nicolas.borghi(at)ijm.fr
RESEARCHMEMBERSPUBLICATIONSNEWSJOB
Research Topics:
Quantitative biology and modeling , Cellular dynamics and signaling, Molecular and cellular pathologies
Selected publications:
1: Déjardin T, Carollo PS, Sipieter F, Davidson PM, Seiler C, Cuvelier D, Cadot
B, Sykes C, Gomes ER, Borghi N. Nesprins are mechanotransducers that
discriminate epithelial-mesenchymal transition programs. J Cell Biol. 2020 Oct
5;219(10):e201908036. doi: 10.1083/jcb.201908036. PMID: 32790861; PMCID:
PMC7659719.
2: Davidson PM, Battistella A, Déjardin T, Betz T, Plastino J, Borghi N, Cadot
B, Sykes C. Nesprin-2 accumulates at the front of the nucleus during confined
cell migration. EMBO Rep. 2020 Jul 3;21(7):e49910. doi: 10.15252/embr.201949910.
Epub 2020 May 17. PMID: 32419336; PMCID: PMC7332974.
3: Audugé N, Padilla-Parra S, Tramier M, Borghi N, Coppey-Moisan M. Chromatin
condensation fluctuations rather than steady-state predict chromatin
accessibility. Nucleic Acids Res. 2019 Jul 9;47(12):6184-6194. doi:
10.1093/nar/gkz373. PMID: 31081027; PMCID: PMC6614833.
4: De Pascalis C, Pérez-González C, Seetharaman S, Boëda B, Vianay B, Burute M,
Leduc C, Borghi N, Trepat X, Etienne-Manneville S. Intermediate filaments
control collective migration by restricting traction forces and sustaining cell-
cell contacts. J Cell Biol. 2018 Sep 3;217(9):3031-3044. doi:
10.1083/jcb.201801162. Epub 2018 Jul 6. PMID: 29980627; PMCID: PMC6122997.
5: Gayrard C, Bernaudin C, Déjardin T, Seiler C, Borghi N. Src- and confinement-
dependent FAK activation causes E-cadherin relaxation and β-catenin activity. J
Cell Biol. 2018 Mar 5;217(3):1063-1077. doi: 10.1083/jcb.201706013. Epub 2018
Jan 8. PMID: 29311227; PMCID: PMC5839785.
6: Sarangi BR, Gupta M, Doss BL, Tissot N, Lam F, Mège RM, Borghi N, Ladoux B.
Coordination between Intra- and Extracellular Forces Regulates Focal Adhesion
Dynamics. Nano Lett. 2017 Jan 11;17(1):399-406. doi:
10.1021/acs.nanolett.6b04364. Epub 2016 Dec 23. PMID: 27990827; PMCID:
PMC5423523.
7: Liu Z, Bun P, Audugé N, Coppey-Moisan M, Borghi N. Vinculin head-tail
interaction defines multiple early mechanisms for cell substrate rigidity
sensing. Integr Biol (Camb). 2016 Jun 13;8(6):693-703. doi: 10.1039/c5ib00307e.
Epub 2016 May 11. PMID: 27169142.
8: Lowndes M, Rakshit S, Shafraz O, Borghi N, Harmon RM, Green KJ, Sivasankar S,
Nelson WJ. Different roles of cadherins in the assembly and structural integrity
of the desmosome complex. J Cell Sci. 2014 May 15;127(Pt 10):2339-50. doi:
10.1242/jcs.146316. Epub 2014 Mar 7. PMID: 24610950; PMCID: PMC4021477.
9: Borghi N, Sorokina M, Shcherbakova OG, Weis WI, Pruitt BL, Nelson WJ, and
E-cadherin is under constitutive actomyosin-generated tension that is
increased at cell-cell contacts upon externally applied stretch. Proc Natl Acad
Sci U S A. 2012 Jul 31;109(31):12568-73. doi: 10.1073/pnas.1204390109. Epub 2012
Jul 16. Erratum in: Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):19034. PMID:
22802638; PMCID: PMC3411997.
10: Borghi N, Lowndes M, Maruthamuthu V, Gardel ML, Nelson WJ. Regulation of
cell motile behavior by crosstalk between cadherin- and integrin-mediated
adhesions. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13324-9. doi:
10.1073/pnas.1002662107. Epub 2010 Jun 21. PMID: 20566866; PMCID: PMC2922157.