Mechanotransduction: from Cell Surface to Nucleus


How our cells respond to their mechanical environment?

We propose an approach using quantitative fluorescence microscopy and genetic engineering for responses from the molecule to the tissue.


Group Leader: Nicolas Borghi

Directeur de Recherche CNRS

+33 (0)157278041





Research Topics:


Quantitative biology and modeling , Cellular dynamics and signaling, Molecular and cellular pathologies


Selected publications:


1: Déjardin T, Carollo PS, Sipieter F, Davidson PM, Seiler C, Cuvelier D, Cadot

B, Sykes C, Gomes ER, Borghi N. Nesprins are mechanotransducers that

discriminate epithelial-mesenchymal transition programs. J Cell Biol. 2020 Oct

5;219(10):e201908036. doi: 10.1083/jcb.201908036. PMID: 32790861; PMCID:


2: Davidson PM, Battistella A, Déjardin T, Betz T, Plastino J, Borghi N, Cadot

B, Sykes C. Nesprin-2 accumulates at the front of the nucleus during confined

cell migration. EMBO Rep. 2020 Jul 3;21(7):e49910. doi: 10.15252/embr.201949910.

Epub 2020 May 17. PMID: 32419336; PMCID: PMC7332974.

3: Audugé N, Padilla-Parra S, Tramier M, Borghi N, Coppey-Moisan M. Chromatin

condensation fluctuations rather than steady-state predict chromatin

accessibility. Nucleic Acids Res. 2019 Jul 9;47(12):6184-6194. doi:

10.1093/nar/gkz373. PMID: 31081027; PMCID: PMC6614833.

4: De Pascalis C, Pérez-González C, Seetharaman S, Boëda B, Vianay B, Burute M,

Leduc C, Borghi N, Trepat X, Etienne-Manneville S. Intermediate filaments

control collective migration by restricting traction forces and sustaining cell-

cell contacts. J Cell Biol. 2018 Sep 3;217(9):3031-3044. doi:

10.1083/jcb.201801162. Epub 2018 Jul 6. PMID: 29980627; PMCID: PMC6122997.

5: Gayrard C, Bernaudin C, Déjardin T, Seiler C, Borghi N. Src- and confinement-

dependent FAK activation causes E-cadherin relaxation and β-catenin activity. J

Cell Biol. 2018 Mar 5;217(3):1063-1077. doi: 10.1083/jcb.201706013. Epub 2018

Jan 8. PMID: 29311227; PMCID: PMC5839785.

6: Sarangi BR, Gupta M, Doss BL, Tissot N, Lam F, Mège RM, Borghi N, Ladoux B.

Coordination between Intra- and Extracellular Forces Regulates Focal Adhesion

Dynamics. Nano Lett. 2017 Jan 11;17(1):399-406. doi:

10.1021/acs.nanolett.6b04364. Epub 2016 Dec 23. PMID: 27990827; PMCID:


7: Liu Z, Bun P, Audugé N, Coppey-Moisan M, Borghi N. Vinculin head-tail

interaction defines multiple early mechanisms for cell substrate rigidity

sensing. Integr Biol (Camb). 2016 Jun 13;8(6):693-703. doi: 10.1039/c5ib00307e.

Epub 2016 May 11. PMID: 27169142.

8: Lowndes M, Rakshit S, Shafraz O, Borghi N, Harmon RM, Green KJ, Sivasankar S,

Nelson WJ. Different roles of cadherins in the assembly and structural integrity

of the desmosome complex. J Cell Sci. 2014 May 15;127(Pt 10):2339-50. doi:

10.1242/jcs.146316. Epub 2014 Mar 7. PMID: 24610950; PMCID: PMC4021477.

9: Borghi N, Sorokina M, Shcherbakova OG, Weis WI, Pruitt BL, Nelson WJ, and Alexander R. Dunn

E-cadherin is under constitutive actomyosin-generated tension that is

increased at cell-cell contacts upon externally applied stretch. Proc Natl Acad

Sci U S A. 2012 Jul 31;109(31):12568-73. doi: 10.1073/pnas.1204390109. Epub 2012

Jul 16. Erratum in: Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):19034. PMID:

22802638; PMCID: PMC3411997.

10: Borghi N, Lowndes M, Maruthamuthu V, Gardel ML, Nelson WJ. Regulation of

cell motile behavior by crosstalk between cadherin- and integrin-mediated

adhesions. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13324-9. doi:

10.1073/pnas.1002662107. Epub 2010 Jun 21. PMID: 20566866; PMCID: PMC2922157.