The Borghi Lab published a new article in PLoS Computational Biology: Deep learning detection of dynamic exocytosis events in fluorescence TIRF microscopy   Abstract: Segmentation and detection of biological objects in fluorescence microscopy is of paramount importance in cell imaging. Deep learning approaches have recently shown promise to advance, automatize and accelerate analysis. However, most of the interest…

The Léon Lab published, in collaboration with the  ProtéoSeine platform, a new article in Plos Genetics: A genetic screen reveals a key role for Reg1 in 2-deoxyglucose sensing and yeast AMPK inhibition   Abstract: The yeast Saccharomyces cerevisiae thrives in sugar-rich environments by rapidly consuming glucose and favoring alcoholic fermentation. This strategy is tightly regulated by the glucose repression pathway,…

The ImagoSeine plaftorm contributed to the publication of a new article in Communication Biology: Myocardial disarray drives metabolic inefficiency in human cardiomyocytes   Abstract: Adult cardiomyocytes are embedded within a highly organized myocardial microenvironment that imposes critical geometric cues essential for the alignment and distribution of organelles and the shaping of their unique, rectangular cellular morphology. Despite the…

The Raote Lab contributed to the publication of a new article in Nature reviews molecular cell biology: Towards a unified framework for the function of endoplasmic reticulum exit sites Résumé: Endoplasmic reticulum exit sites (ERES) are specialized, ribosome-free ER subdomains that serve as dynamic portals for COPII-mediated export of proteins from the ER. Beyond their role…

The ProtéoSeine platform contributed to the publication of a new article in Cell Host & Microbe: Immunometabolic reprogramming of macrophages by gut microbiota-derived cadaverine controls colon inflammation   Abstract: Cadaverine is a polyamine produced by the gut microbiota with links to health and disease, notably inflammatory bowel disease (IBD). Here, we show that cadaverine shapes monocyte-macrophage immunometabolism…

The Greenberg Lab published a new review in Plos Genetics: Epigenetic relay: Polycomb-directed DNA methylation in mammalian development Abstract: In mammals, repression of germline-specific gene expression is essential for preserving somatic cell identity and preventing disease. Germline gene silencing is often dependent on the presence of promoter 5-cytosine DNA methylation (5meC). Early mammalian development, however, is marked…

The Romet-Lemonne/Jégou Lab contributed to the publication of a new article in Nature structural & molecular biology: Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers Abstract: Branched actin networks nucleated by the Arp2/3 complex have critical roles in various cellular processes, from cell migration to intracellular transport. However, when activated by WISH/DIP/SPIN90-family proteins, Arp2/3 nucleates linear actin filaments.…

The Ladoux/Mege Lab contributed to the publication of a new article in Nature Communications: Capturing nematic order on tissue surfaces of arbitrary geometry   Abstract: A leading paradigm for understanding the large-scale behavior of tissues is via generalizations of liquid crystal physics; much like liquid crystals, tissues combine fluid-like, viscoelastic behaviors with local orientational order, such as nematic…

The Romet-Lemonne/Jégou Lab published a new nouvel article in The European Physical Journal E: Probing protein–protein interactions with drag flow: a case study of F-actin and tropomyosin Abstract: Tropomyosins are central regulators of the actin cytoskeleton, controlling the binding and activity of the other actin binding proteins. The interaction between tropomyosin and actin is quite unique:…

L'équipe Greenberg a contribué à la publication d'un nouvel article dans Nature Communications : UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells   Abstract: In mammals, primordial germ cells (PGCs) undergo global erasure of DNA methylation with delayed demethylation of germline genes and selective retention of DNA methylation at evolutionarily young retrotransposons. However, the molecular mechanisms of…