Skip to content Skip to footer

Institut Jacques Monod
Close

Aude-Isabelle Dupré

 

  • 2022- now : CNRS Research Director, Mechanism of Meiosis’s team held by K. Wassmann, Institut Jacques Monod, Paris, France
  • 2007-2021 : CNRS Research Scientist, Biology of Oocyte’s team held by C. Jessus, IBPS, Paris, France
  • 2002-2007 : Post-doctoral researcher, Institute for Cancer Genetics, J. Gautier’s team, Columbia University, NY, USA
  • 1999-2002 : PhD, Physiology of Reproduction, Sorbonne University (UPMC), Paris, France

 

Meiosis is fundamental to sexual reproduction, ensuring the formation of haploid gametes. It consists of two successive divisions, meiosis I and meiosis II, with no intermediate S-phase. Moreover, in females, it is characterized by two physiological arrests. Oocytes are blocked in prophase of meiosis I to prepare for embryonic development, and then in metaphase of meiosis II while awaiting fertilization. Although meiotic progression and arrests remain poorly understood, they rely on the activity and specificity of the Cdk1 kinase, associated with cyclin B1, B2, and B3. The periodic accumulation/degradation of B1/B2 orchestrate the succession of the two divisions, while the turnover of B3 enables oocytes to distinguish meiosis I from meiosis II. Cyclin B3 is indeed essential for the metaphase I- to – anaphase I transition, and its degradation at the end of meiosis I is crucial to establish and maintain the metaphase II arrest. This project aims to elucidate the molecular mechanisms governing the late degradation of B3, to characterize its functional role between the two meiotic divisions, and to identify its substrates in order to better understand Cdk1 functional specificity. It relies on two complementary oocyte models: the Xenopus frog, for detailed biochemical analysis of meiosis, and the mouse, to study the conservation of these regulatory mechanisms in mammals. This integrated approach should provide a deeper mechanistic and evolutionary understanding of the processes that govern proper female meiosis.

 

  • 2025-2029 : ANR-AAPG 2024
  • 2022-2024 : Project ARC Fondation PJA2
  • 2018-2021 : ANR-AAPG 2018
  • 2017-2019 : Project ARC Fondation PJA2
  • 2011-2014 : Emergence » UPMC Project

 

  • Stefania Castagnetti (LBDV, Villefranche-sur mer, France)
  • Evelyn Houliston (LBDV, Villefranche-sur mer, France)

 

  • 3 PhD students: Enrico Daldello (2011-2015), Tom Lemonnier (2016-2019), Emmanuelle Marjault (2025-2028)
  • 14 Master students, 2 License students, 1 Engineer

 

Article

2022    Nikalayevich, E., El Jailani, S., Dupré, A., Cladière, D., Gryaznova, Y., Fosse, C., Buffin, E., Touati, S., Wassmann, K. Aurora B/C-dependent phosphorylation promotes Rec8 cleavage in mammalian oocytes. Current Biology 32(10):2281-2290.e4. doi: 10.1016/j.cub.2022.03.041.

2021    Lemonnier, T.1, Daldello, E.M.1, Poulhe, R., Le, T., Miot M., Jessus, C.2 and Dupré, A. 2,*. The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division. Nature Communications 12, 1837, doi: 10.1038/s41467-021-22124-0. 1: Co-1st, 2: Co-last.

2017    Dupré, A.*, Haccard, O., and Jessus, C. The greatwall kinase is dominant over PKA in controlling the antagonistic function of ARPP19 in Xenopus oocytes. Cell Cycle (16): 1440-52. doi: 10.1080/15384101.2017.1338985

2015    Daldello, E.M., Le, T, Poulhe, R, Jessus, C., Haccard, O. and Dupré, A.* Control of Cdc6 accumulation by Cdk1 and MAPK is essential for completion of oocyte meiotic divisions in Xenopus. Journal of Cell Science (14):2482-96. doi: 10.1242/jcs.166553

2014    Dupré, A.1, Daldello, E.M.1, Nairn, A.C., Jessus, C., and Haccard, O. Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes. Nature Communications 5, 3318. 1: Co-1st. doi: 10.1038/ncomms4318

2013    Dupré, A., Buffin, E., Roustan, C., Nairn, A.C., Jessus, C., and Haccard, O. The phosphorylation of ARPP19 by Greatwall renders the auto-amplification of MPF independently of PKA in Xenopus oocytes. Journal of Cell Science (126): 3916-26. doi: 10.1242/jcs.126599

2012    Haccard, O., Dupré, A., Liere, P., Pianos, A., Eychenne, B., Jessus, C., and Ozon, R. Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates. Molecular and Cellular Endocrinology (362): 110-119. doi: 10.1016/j.mce.2012.05.019

2011    Du Pasquier, D., Dupré, A. and Jessus, C. Unfertilized Xenopus eggs die by Bad-dependent apoptosis under the control of Cdk1 and JNK. PLoS One, doi: 10.1371/journal.pone.0023672

2008    Dupré, A., Boyer-Chatenet, L., Sattler, R.M., Modi, A.P., Lee, J.H., Nicolette, M.L., Kopelovich, L., Jasin, M., Baer, R., Paull, T.T. and Gautier J. A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nature Chemical Biology (4): 119-25. doi: 10.1038/nchembio.63

2007    Bhaskara, V., Dupré, A., Lengsfeld, B., Hopkins, B.B., Chan, A., Lee, J.H., Zhang, X., Gautier, J., Zakian, V. and Paull, T.T. Rad50 adenylate kinase activity regulates DNA tethering by Mre11/Rad50 complexes. Molecular Cell (25): 647-61. doi: 10.1016/j.molcel.2007.01.028

2006    Dupré, A., Boyer-Chatenet, L., and Gautier, J. Two-step activation of ATM by DNA and the Mre11-Rad50-Nbs1 complex. Nature Structural and Molecular Biology (13): 451-57. doi 10.1016/j.molcel.2007.01.028

2006    Gaffré, M., Dupré, A., Valuckaite, R., Suziedelis, K., Jessus, C. and Haccard, O. Deciphering the H-Ras pathway in Xenopus oocyte. Oncogene (25): 5155-62. doi: 10.1038/sj.onc.1209523

2002    Dupré, A., Jessus, C., Ozon, R. and Haccard, O. Mos is not required for the initiation of meiotic maturation in Xenopus oocytes. EMBO Journal (21): 4026-36. doi: 10.1093/emboj/cdf400

2002    Dupré, A., Suziedelis, K., Valuckaite, R., De Gunzburg, J., Ozon, R., Jessus, C. and Haccard, O. Xenopus H-RasV12 promotes entry into meiotic M phase and cdc2 activation independently of Mos and p42(MAPK). Oncogene (21): 6425-33. doi: 10.1093/emboj/cdf400

Review

2023    Dupré, A.* and Wassmann, K.* La cycline B3, verrou de la méiose femelle en attendant la fécondation. Med Sci (Paris), 39(3), doi : https:// doi.org/10.1051/medsci/2023019.

2020    Meneau, F., Dupré, A., Jessus, C. and Daldello, E.M. Translational Control of Xenopus Oocyte Meiosis: Toward the Genomic Era. Cells. 9(6):1502. doi: 10.3390/cells9061502

2020    Lemonnier, T., Dupré, A. and Jessus, C. The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division. Cell Division (15):9. doi: 10.1186/s13008-020-00065-2. Equal contribution

2011    Dupré, A., Haccard, O. and Jessus, C. Mos in the oocyte: how to use MAPK independently of growth factors and transcription to control meiotic divisions. Journal of Signal Transduction, 350412. doi: 10.1155/2011/350412

2007    Ben-Yehoyada, M., Gautier, J., Dupré, A*. The DNA damage response during an unperturbed S-phase. DNA repair (6)7:914-922. doi: 10.1016/j.dnarep.2007.02.005

2001    Karaiskou, A., Dupré, A., Haccard, O. and Jessus, C. From progesterone to active Cdc2 in Xenopus oocytes: a puzzling signalling pathway. Biology of the Cell (93) : 35-46. doi: 10.1016/s0248-4900(01)01126-1

Book chapter

2025    Dupré, A.* and wassmann K. Cell cycle regulation of vertebrate female meiosis. B. Mogessie (ed.), Cellular Architecture and Dynamics in Female Meiosis, https://doi.org/10.1007/978-3-031-97173-0_4

2017    Dupré, A. and Jessus, C. ARPP19 Phosphorylations by PKA and Greatwall: The Yin and the Yang of the Cell Decision to Divide. Protein phosphorylation, C. Prigent (ed.) (InTechOpen). doi:10.5772/INTECHOPEN.71332

 

  • 2023 – now : Member of the CN3 ARC commission
  • 2022 – now : Co-responsability of Experimentation Animal Module « Aquatic Models » (IBPS, SU)
  • 2016 – 2021 : Member of section 22 of the CoNRS
  • 2014 – 2019 : Member of the Unit Council (LBD, SU).