Contract Period : 24 months
Expected date of employment : 1 April 2024
Apply before 1st of December 2023 on: https://emploi.cnrs.fr/Offres/CDD/UMR7592-JEAFER-002/Default.aspx?lang=EN
Missions
Managing a research project
Objective: creating a Vibrio cholerae strain adapted to DnaC, tracing the modifications and their succession during adaptation to DnaC to understand the mechanism underlying bidirectional replication initiation.
Activities
– Setting up the evolutionary screen assay
– Construction of the screen strain
– Construction of the plasmids of complementation
– Building a viable screen strain
– Steady state culture of cells and selection of cells with improved fitness
– Whole genome sequence of adapted cells
– Bioinformatics to identify the adaptive mutations
Skills
– Molecular biology (needed)
– Genetics (needed)
– Bioinformatics (would be a plus – formation possible)
– Evolutionary biology (would be a plus)
– Cytology (would be a plus)
Work context
The host team
The team comprises 3 permanent positions and 3 PhD students, working on the initiation of replication in vertebrates (human, chicken) and bacteria (Vibrio cholerae, Escherichia coli). The project will be supervised by Jean-Luc Ferat who is a professor at University Paris Cité.
The IJM
The Institut Jacques Monod is a joint research unit of CNRS and Université Paris Cité. The Institute is ideally located on the left bank of Paris. The institute is a fundamental research center with some thirty groups covering the fields of genome and chromosome dynamics, cell dynamics and signaling, development and evolution with a rich scientific life.
