LSM 780 Inverted confocal

User Manual

Capabilities

• High sensitivity point scanning confocal imaging
• Spectral array GaAsP detectors
• Inverted microscope
• Incubation for live cell timelapse – temp and CO₂ control
• Timelapse with Definite focus2
• Multi-position
• Tiles acquisition and stitching of images
• BF and Nomarski contrast (DIC)

Detailed Specifications

Microscope:
Axio Observer Z.1 Fully motorized Inverted stand
Motorized XY stage

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Pecon)

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 476, 488, 496, 514nm)
• 561 nm Diode laser
• HeNe 633nm

Objectives:

• 10X/0.5 Plan-NeoFl Dry (420140-9900)
• 25x/0.8 LD LCI Plan-Apochromat Imm Corr DIC (420852-9871)
• 40X/1.4 Plan-Apochromat Oil DIC (420762-9800)
• 63X/1.4 Plan-Apochromat Oil DIC (420782-9900)

Dichroic(s):

• Twin gate dichroic system
• Low angle of incidence for improved reflection suppression

Detectors:

• GaAsP high QE 32 channel spectral array with unmixing capability
• 2 standard alkali PMTs in positions for blue and far-red detection
• Transmitted light detector

Computer:

• Z840 (HP)
• Intel Xeon E5-2623 CPU 2,99 GHZ; 192 GB RAM
• Acquisition Software ZEN black 2.3