IJM Seminar – Gaëlle Legube – 28/06/2024

IJM Seminar – Gaëlle Legube – 28/06/2024

Invited by par the Duharcourt, Gaëlle Legube (CBI, Toulouse) will present an Institut Jacques Monod seminar on the theme:

Chromosome and chromatin dynamics at DNA double strand breaks

 

Abstract:

DNA double-strand breaks (DSBs) are highly toxic lesions that are rapidly repaired by two main pathways, namely Homologous Recombination (HR) and Non Homologous End Joining (NHEJ). Using a cell line, called DIvA (for DSB Inducible via AsiSI), where multiples breaks are induced at annotated positions, combined with genome-wide, high throughput sequencing based techniques (ChIP-seq, HiC…) we investigate the contribution of chromatin and chromosome conformation in the response to DSB. I will present our recent work, on the contribution of chromatin and Topologically Associating Domains (TADs) during DSB repair. More specifically we recently demonstrated the role of cohesin-mediated loop extrusion in establishing gammaH2AX on an entire TAD in cis to DSBs. We now provide evidence that when established, gammaH2AX “tagged” TAD further self-segregate in the nucleus, forming a novel DSB-induced chromatin compartment (the “D” compartment) that contributes to the activation of the DNA damage response.

 

The seminar will take place on Friday, June 28th at 11.45 am in room François Jacob (15 rue Hélène Brion 75013 Paris)