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[Annulé] Séminaire IJM – Gaëlle Legube – 28/06/2024

Le séminaire est annulé

Invitée par l’équipe Duharcourt, Gaëlle Legube (CBI, Toulouse) présentera un séminaire de l’Institut Jacques Monod sur le thème :

Chromosome and chromatin dynamics at DNA double strand breaks

 

Résumé :

DNA double-strand breaks (DSBs) are highly toxic lesions that are rapidly repaired by two main pathways, namely Homologous Recombination (HR) and Non Homologous End Joining (NHEJ). Using a cell line, called DIvA (for DSB Inducible via AsiSI), where multiples breaks are induced at annotated positions, combined with genome-wide, high throughput sequencing based techniques (ChIP-seq, HiC…) we investigate the contribution of chromatin and chromosome conformation in the response to DSB. I will present our recent work, on the contribution of chromatin and Topologically Associating Domains (TADs) during DSB repair. More specifically we recently demonstrated the role of cohesin-mediated loop extrusion in establishing gammaH2AX on an entire TAD in cis to DSBs. We now provide evidence that when established, gammaH2AX “tagged” TAD further self-segregate in the nucleus, forming a novel DSB-induced chromatin compartment (the “D” compartment) that contributes to the activation of the DNA damage response.

 

Le séminaire aura lieu vendredi 28 juin de 11h45 à 13h en salle François Jacob (15 rue Hélène Brion 75013 Paris)

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