[Cancelled] IJM Seminar – Gaëlle Legube

The seminar is cancelled

Invited by par the Duharcourt, Gaëlle Legube (CBI, Toulouse) will present an Institut Jacques Monod seminar on the theme:

Chromosome and chromatin dynamics at DNA double strand breaks

Abstract:

DNA double-strand breaks (DSBs) are highly toxic lesions that are rapidly repaired by two main pathways, namely Homologous Recombination (HR) and Non Homologous End Joining (NHEJ). Using a cell line, called DIvA (for DSB Inducible via AsiSI), where multiples breaks are induced at annotated positions, combined with genome-wide, high throughput sequencing based techniques (ChIP-seq, HiC…) we investigate the contribution of chromatin and chromosome conformation in the response to DSB. I will present our recent work, on the contribution of chromatin and Topologically Associating Domains (TADs) during DSB repair. More specifically we recently demonstrated the role of cohesin-mediated loop extrusion in establishing gammaH2AX on an entire TAD in cis to DSBs. We now provide evidence that when established, gammaH2AX “tagged” TAD further self-segregate in the nucleus, forming a novel DSB-induced chromatin compartment (the “D” compartment) that contributes to the activation of the DNA damage response