ELYRA 7 SIM STORM

 

User Manual

The Basic Principle of Lattice SIM

The Basic Principle of SMLM

The Basic Principle of TIRF

 

Capabilities

  • Super resolution 3D Lattice SIM imaging for fixed or living sample
  • Super resolution 3D PALM imaging for living sample
  • Super resolution 3D dSTORM imaging for fixed sample
  • TIRF imaging
  • Inverted microscope
  • Incubation for live cell timelapse – temp and CO2 control
  • IR reflection based autofocus

Detailed Specifications

Zeiss Axio Observer7 stand with Definite Focus3
Motorized Piezo stage 130 mm x 100 mm
Piezo Z stage 500µm

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cube

Full environmental chamber with heating, humidity and CO2 control (Pecon)

Lasers:
405 nm (50mW), 488 nm (500mW), 561nm (500mW), 639nm (500mW)

Objectives:

  • 10x/0,20 Plan-Neofluar M27 (420340-9901)
  • 20x/0.8 Plan-Apochromat M27 (420650-9903)
  • 40x/1.4 Plan-Apochromat Oil DIC M27 (420762-9900)
  • 63x/1,4 Plan-Apochromat Oil DIC M27 (420762-9900-799)
  • 63x/1,46 Plan-Apochromat Oil Korr M27 Var2 (420780-9971)

Detectors:
Two cameras Edge 4.2 CLHS (PCO), adjusted for simultaneous display of dual channel

Workstation Z6 G4 (HP)
Xeon Gold 6234 3.3GHZ; 196Go RAM
SW ZEN Black 3.4