Collignon Lab – Dissecting signaling hierarchies in the patterning of the mouse primitive streak using micropatterned EpiLC colonies

Collignon Lab – Dissecting signaling hierarchies in the patterning of the mouse primitive streak using micropatterned EpiLC colonies
Congratulations to the Collignon Team for this new paper just published in Stem Cell Reports!

Summary

Embryo studies have established that the patterning of the mouse gastrula depends on a regulatory network in which the WNT, BMP, and NODAL signaling pathways cooperate, but aspects of their respective contributions remain unclear. Studying their impact on the spatial organization and developmental trajectories of micropatterned epiblast-like cell (EpiLC) colonies, we show that NODAL is required prior to BMP action to establish the mesoderm and endoderm lineages. The presence of BMP then forces NODAL and WNT to support the formation of posterior primitive streak (PS) derivatives, while its absence allows them to promote that of anterior PS derivatives. Also, a Nodal mutation elicits more severe patterning defects in vitro than in the embryo, suggesting that ligands of extra-embryonic origin can rescue them. These results support the implication of a combinatorial process in PS patterning and illustrate how the study of micropatterned EpiLC colonies can complement that of embryos.

Keywords

micropatterned EpiLC colonies
primitive streak
mouse
differentiation

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