Cell Adhesion and Mechanics


Mechanical constraints and force transmission play an essential role in multicellular living organisms They are regulating basic biological processes such as morphogenesis, tumor metastasis and tissue repair. Cell adhesions, coupled to the contractile cytoskeleton, are major sites of force transmission in cells. This mechanical coupling which enables cells to sense, signal, and respond to physical changes in the environment, has however been largely understudied. In this context, we are studying the cooperation between adhesion, mechanical and biochemical signaling for the adaptation of living cells to changes in their physical environment at various scales, from single molecules to tissues.

Keywords: mechanobiology, mechanosensing, epithelia homeostasis, collective cell migration, microfabrication, biophysics, cell extrusion, tissue mechanics

+33 (0)157278071 /  +33 (0)157278067     benoit.ladoux(at)ijm.fr  /  rene-marc.mege(at)ijm.fr     @BLadoux     @ReneMege    



We study force sensing and mechanotransduction at integrin-mediated cell-extracellular matrix and cadherin-mediated cell-cell adhesions. To answer these questions, we have developed single cells and cell doublets models allowing a tight control of cell-matrix and cell-adhesion formation in a physically and mechanically defined microenvironment. Coupled with advanced microscopy, microforce sensor devices and classical cell biology, these approaches allow us to determine the molecular mechanisms that control cell adhesion and cytoskeleton remodeling, as well as cell shape and migration and their adaptation to environment compliance as well as to cytoskeleton visco-elastic properties and cell’s internal tension generated by myosin motors.




We study the collective behavior of cells in epithelial sheets, in the context of tissue homeostasis and wound healing. To answer these questions, we developing microfabricated tools   and biophysical tools to measure and control the mechanical properties and topology of cell’s microenvironment. These tools are combined with molecular approaches, advanced techniques in light microscopy, image analysis and modeling to study the influence of physical properties of the environment on the organization of epithelial layers, collective cell migration, single and collective cell polarization, cell division and cell extrusion. We are characterizing how physical constraints can lead to emergent dynamical and mechanical properties of various epithelial tissues.





We study how more complex epithelial tissues formed of mixed populations of cells (normal/adhesion deficient, normal/cancer, differentiation/stem cells) facing homogeneous and heterogeneous substrates (extracellular matrix chemistry, rigidity, geometry and topography), regulate homeostasis, segregate and/or auto assemble. Our aims are to determine i) how physical constraints of the microenvironment modulate mechanical properties of epithelial cells and tissues, ii) how they direct a variety of cell behaviors including stem cell proliferation, cell extrusion or delamination, cell migration, differentiation, and polarity, and iii) how they impact on the morphogenesis normal epithelial tissue, as well as the pathological development of intestinal rare diseases. Biomimetic substrates coupled to high resolution imaging and biochemistry are instrumental to reach these goals.

Group Leaders:

Téléphone : +33 (0)157278071 / Email : benoit.ladoux (at) ijm.fr

René-Marc MÈGE
Téléphone : +33 (0)157278067 / Email : rene-marc.mege (at) ijm.fr


Membres de l’équipe :

ANGER Lucas Engineer
D’ALESSANDRO Joseph Researcher
DE BECO Simon teacher-researcher
DUBEY Sushil Postdoc
FARDIN Marc-Antoine Researcher
DANG Tien Engineer
PENETI Sudheer Kumar PhD Student
ROSSE Carine invited researcher
SCHONIT Andreas PhD student
SHEN Yuan Postdoc
WODRASCKA Fanny PhD Student
WU Huiqiong Postdoc
XI Wang Researcher
CHILUPURI Ranjith Kumar PhD Student
JIANG Pan Postdoctorant
MARTINS Joana PhD Student visitor
VIGNES Hélène Postdoc
GRUDTSYNA Valeriia PhD Student visitor
THIANT Clémence PhD Student
ARKOWITZ Grégory PhD Student
DAI Wufei PhD Student
KAILASAM MANI Satish Postdoc
RACHIDI Joud Master 2

Balasubramaniam L, Doostmohammadi A, Saw TB, Narayana GHNS, Mueller R, Dang T, Thomas M, Gupta S, Sonam S, Yap AS, Toyama Y, Mège RM, Yeomans JM, Ladoux B. Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nat Mater. 2021 Aug;20(8):1156-1166. doi: 10.1038/s41563-021-00919-2. Epub 2021 Feb 18. Erratum in: Nat Mater. 2021 Mar 9;: PMID: 33603188; PMCID: PMC7611436.

Gaston C, De Beco S, Doss B, Pan M, Gauquelin E, D’Alessandro J, Lim CT, Ladoux B, Delacour D. EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nat Commun. 2021 Apr 13;12(1):2226. doi: 10.1038/s41467-021-22482-9. PMID: 33850145; PMCID: PMC8044225.

Jain S, Cachoux VML, Narayana GHNS, de Beco S, D’Alessandro J, Cellerin V, Chen T, Heuzé ML, Marcq P, Mège RM, Kabla AJ, Lim CT, Ladoux B. The role of single cell mechanical behavior and polarity in driving collective cell migration. Nat Phys. 2020 Jul;16(7):802-809. doi: 10.1038/s41567-020-0875-z. Epub 2020 May 4. PMID: 32641972; PMCID: PMC7343533.

Le AP, Rupprecht JF, Mège RM, Toyama Y, Lim CT, Ladoux B. Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nat Commun. 2021 Jan 15;12(1):397. doi: 10.1038/s41467-020-20563-9. PMID: 33452264; PMCID: PMC7810754.

Doss BL, Pan M, Gupta M, Grenci G, Mège RM, Lim CT, Sheetz MP, Voituriez R, Ladoux B. Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress. Proc Natl Acad Sci U S A. 2020 Jun 9;117(23):12817-12825.
doi: 10.1073/pnas.1917555117. Epub 2020 May 22. PMID: 32444491; PMCID: PMC7293595.

Heuzé ML, Sankara Narayana GHN, D’Alessandro J, Cellerin V, Dang T, Williams DS, Van Hest JC, Marcq P, Mège RM, Ladoux B. Myosin II isoforms play distinct roles in <i>adherens</i> junction biogenesis. Elife. 2019 Sep 5;8:e46599. doi: 10.7554/eLife.46599. PMID: 31486768; PMCID: PMC6756789.

Chen T, Callan-Jones A, Fedorov E, Ravasio A, Brugués A, Ong HT, Toyama Y, Low BC, Trepat X, Shemesh T, Voituriez R, Ladoux B. Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nat Phys. 2019 Apr;15:393-402. doi: 10.1038/s41567-018-0383-6. Epub 2019 Jan 21. PMID: 30984281; PMCID: PMC6456019.

Seddiki R, Narayana GHNS, Strale PO, Balcioglu HE, Peyret G, Yao M, Le AP, Teck Lim C, Yan J, Ladoux B, Mège RM. Force-dependent binding of vinculin to α-catenin regulates cell-cell contact stability and collective cell behavior.
Mol Biol Cell. 2018 Feb 15;29(4):380-388. doi: 10.1091/mbc.E17-04-0231. Epub 2017 Dec 27. PMID: 29282282; PMCID: PMC6014167.

Saw TB, Doostmohammadi A, Nier V, Kocgozlu L, Thampi S, Toyama Y, Marcq P, Lim CT, Yeomans JM, Ladoux B. Topological defects in epithelia govern cell death and extrusion. Nature. 2017 Apr 12;544(7649):212-216.

Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, Mayeux A, Michaux G, Campeotto F, Lemale J, Viala J, Poirier F, Minc N, Schmitz J, Brousse N, Ladoux B, Goulet O, Delacour D. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017 Jan 13;8:13998.


Bertrand, T., d’Alessandro, J., Maitra, A., Jain, S., Mercier, B., Mège, R.-M., Ladoux, B., & Voituriez, R. (2024). Clustering and ordering in cell assemblies with generic asymmetric aligning interactions. Physical Review Research, 6(2), 023022. https://doi.org/10.1103/PhysRevResearch.6.023022
Sadhu, R. K., Luciano, M., Xi, W., Martinez-Torres, C., Schröder, M., Blum, C., Tarantola, M., Villa, S., Penič, S., Iglič, A., Beta, C., Steinbock, O., Bodenschatz, E., Ladoux, B., Gabriele, S., & Gov, N. S. (2024). A minimal physical model for curvotaxis driven by curved protein complexes at the cell’s leading edge. Proceedings of the National Academy of Sciences, 121(12), e2306818121. https://doi.org/10.1073/pnas.2306818121
Monteiro, P., Remy, D., Lemerle, E., Routet, F., Macé, A.-S., Guedj, C., Ladoux, B., Vassilopoulos, S., Lamaze, C., & Chavrier, P. (2023). A mechanosensitive caveolae–invadosome interplay drives matrix remodelling for cancer cell invasion. Nature Cell Biology, 1–17. https://doi.org/10.1038/s41556-023-01272-z
Eckert, J., Ladoux, B., Mège, R.-M., Giomi, L., & Schmidt, T. (2023). Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell density. Nature Communications, 14(1), 5762. https://doi.org/10.1038/s41467-023-41449-6
Lignieres, L., Sénécaut, N., Dang, T., Bellutti, L., Hamon, M., Terrier, S., Legros, V., Chevreux, G., Lelandais, G., Mège, R.-M., Dumont, J., & Camadro, J.-M. (2023). Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling. Journal of Proteome Research, 22(3), 996–1002. https://doi.org/10.1021/acs.jproteome.2c00699
Kawaue, T., Yow, I., Pan, Y., Le, A. P., Lou, Y., Loberas, M., Shagirov, M., Teng, X., Prost, J., Hiraiwa, T., Ladoux, B., & Toyama, Y. (2023). Inhomogeneous mechanotransduction defines the spatial pattern of apoptosis-induced compensatory proliferation. Developmental Cell, 58(4), 267-277.e5. https://doi.org/10.1016/j.devcel.2023.01.005
Rose, N., Estrada Chavez, B., Sonam, S., Nguyen, T., Grenci, G., Bigot, A., Muchir, A., Ladoux, B., Cadot, B., Le Grand, F., & Trichet, L. (2023). Bioengineering a miniaturized in vitro 3D myotube contraction monitoring chip to model muscular dystrophies. Biomaterials, 293, 121935. https://doi.org/10.1016/j.biomaterials.2022.121935
Sonam, S., Balasubramaniam, L., Lin, S.-Z., Ivan, Y. M. Y., Jaumà, I. P., Jebane, C., Karnat, M., Toyama, Y., Marcq, P., Prost, J., Mège, R.-M., Rupprecht, J.-F., & Ladoux, B. (2023). Mechanical stress driven by rigidity sensing governs epithelial stability. Nature Physics, 19, 132–141. https://doi.org/10.1038/s41567-022-01826-2
Gautier, B., Meneux, L., Feret, N., Audrain, C., Hudecek, L., Kuony, A., Bourdon, A., Le Guiner, C., Blouin, V., Delettre, C., & Michon, F. (2022). AAV2/9-mediated gene transfer into murine lacrimal gland leads to a long-term targeted tear film modification. Molecular Therapy. Methods & Clinical Development, 27, 1–16. https://doi.org/10.1016/j.omtm.2022.08.006
Kabla, A., Ladoux, B., & Di Meglio, J.-M. (2022). Tissue mechanics. The European Physical Journal. E, Soft Matter, 45(11), 90. https://doi.org/10.1140/epje/s10189-022-00240-z
d’Alessandro, J., Barbier-Chebbah, A., Voituriez, R., & Ladoux, B. (2022). [Cells as « Tom Thumbs » of living tissues]. Medecine Sciences: M/S, 38(11), 861–863. https://doi.org/10.1051/medsci/2022138
Petracchini, S., Hamaoui, D., Doye, A., Asnacios, A., Fage, F., Vitiello, E., Balland, M., Janel, S., Lafont, F., Gupta, M., Ladoux, B., Gilleron, J., Maia, T. M., Impens, F., Gagnoux-Palacios, L., Daugaard, M., Sorensen, P. H., Lemichez, E., & Mettouchi, A. (2022). Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction to control bacterial invasion and cell division. Nature Communications, 13(1), 6059. https://doi.org/10.1038/s41467-022-33803-x
Glentis, A., Blanch-Mercader, C., Balasubramaniam, L., Saw, T. B., d’Alessandro, J., Janel, S., Douanier, A., Delaval, B., Lafont, F., Lim, C. T., Delacour, D., Prost, J., Xi, W., & Ladoux, B. (2022). The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces. Science Advances, 8(37), eabn5406. https://doi.org/10.1126/sciadv.abn5406
Willems, M., Wells, C. F., Coubes, C., Pequignot, M., Kuony, A., & Michon, F. (2022). Hypolacrimia and Alacrimia as Diagnostic Features for Genetic or Congenital Conditions. Investigative Ophthalmology & Visual Science, 63(9), 3. https://doi.org/10.1167/iovs.63.9.3
Fardin, M. A., Hautefeuille, M., & Sharma, V. (2022). Spreading, pinching, and coalescence: the Ohnesorge units. Soft Matter, 18(17), 3291–3303. https://doi.org/10.1039/d2sm00069e
Liu, X., Tsubota, K., Yu, Y., Xi, W., & Gong, X. (2022). A numerical method to predict the membrane tension distribution of spreading cells based on the reconstruction of focal adhesions. Science China Physics, Mechanics & Astronomy, 65(6), 264612. https://doi.org/10.1007/s11433-021-1873-1
Xi, W., Saleh, J., Yamada, A., Tomba, C., Mercier, B., Janel, S., Dang, T., Soleilhac, M., Djemat, A., Wu, H., Romagnolo, B., Lafont, F., Mège, R.-M., Chen, Y., & Delacour, D. (2022). Modulation of designer biomimetic matrices for optimized differentiated intestinal epithelial cultures. Biomaterials, 282, 121380. https://doi.org/10.1016/j.biomaterials.2022.121380
Yang, Y.-A., Nguyen, E., Sankara Narayana, G. H. N., Heuzé, M., Fu, C., Yu, H., Mège, R.-M., Ladoux, B., & Sheetz, M. P. (2022). Local contractions regulate E-cadherin rigidity sensing. Science Advances, 8(4), eabk0387. https://doi.org/10.1126/sciadv.abk0387
Zisis, T., Brückner, D. B., Brandstätter, T., Siow, W. X., d’Alessandro, J., Vollmar, A. M., Broedersz, C. P., & Zahler, S. (2022). Disentangling cadherin-mediated cell-cell interactions in collective cancer cell migration. Biophysical Journal, 121(1), 44–60. https://doi.org/10.1016/j.bpj.2021.12.006
Sonam, S., Vigouroux, C., Jégou, A., Romet-Lemonne, G., Le Clainche, C., Ladoux, B., & Mège, R. M. (2021). Direct measurement of near-nano-Newton forces developed by self-organizing actomyosin fibers bound α-catenin. Biology of the Cell, 113(11), 441–449. https://doi.org/10.1111/boc.202100014
Jain, S., Ladoux, B., & Mège, R.-M. (2021). Mechanical plasticity in collective cell migration. Current Opinion in Cell Biology, 72, 54–62. https://doi.org/10.1016/j.ceb.2021.04.006
Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mège, R.-M., Yeomans, J. M., & Ladoux, B. (2021). Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1156–1166. https://doi.org/10.1038/s41563-021-00919-2
Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mège, R.-M., Yeomans, J. M., & Ladoux, B. (2021). Author Correction: Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1167. https://doi.org/10.1038/s41563-021-00974-9
d’Alessandro, J., Barbier-Chebbah, A., Cellerin, V., Benichou, O., Mège, R. M., Voituriez, R., & Ladoux, B. (2021). Cell migration guided by long-lived spatial memory. Nature Communications, 12(1), 4118. https://doi.org/10.1038/s41467-021-24249-8
Salvi, A. M., Bays, J. L., Mackin, S. R., Mege, R.-M., & DeMali, K. A. (2021). Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake. Nature Cell Biology, 23(5), 457–466. https://doi.org/10.1038/s41556-021-00677-y
Gaston, C., De Beco, S., Doss, B., Pan, M., Gauquelin, E., D’Alessandro, J., Lim, C. T., Ladoux, B., & Delacour, D. (2021). EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nature Communications, 12(1), 2226. https://doi.org/10.1038/s41467-021-22482-9
Latorre, E., Kale, S., Casares, L., Gómez-González, M., Uroz, M., Valon, L., Nair, R. V., Garreta, E., Montserrat, N., Del Campo, A., Ladoux, B., Arroyo, M., & Trepat, X. (2021). Addendum: Active superelasticity in three-dimensional epithelia of controlled shape. Nature, 592(7856), E30. https://doi.org/10.1038/s41586-021-03281-0
Le, A. P., Rupprecht, J.-F., Mège, R.-M., Toyama, Y., Lim, C. T., & Ladoux, B. (2021). Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nature Communications, 12(1), 397. https://doi.org/10.1038/s41467-020-20563-9
Ghisleni, A., Galli, C., Monzo, P., Ascione, F., Fardin, M.-A., Scita, G., Li, Q., Maiuri, P., & Gauthier, N. C. (2020). Complementary mesoscale dynamics of spectrin and acto-myosin shape membrane territories during mechanoresponse. Nature Communications, 11(1), 5108. https://doi.org/10.1038/s41467-020-18825-7
Tlili, S., Durande, M., Gay, C., Ladoux, B., Graner, F., & Delanoë-Ayari, H. (2020). Migrating Epithelial Monolayer Flows Like a Maxwell Viscoelastic Liquid. Physical Review Letters, 125(8), 088102. https://doi.org/10.1103/PhysRevLett.125.088102
Teo, J. L., Gomez, G. A., Weeratunga, S., Davies, E. M., Noordstra, I., Budnar, S., Katsuno-Kambe, H., McGrath, M. J., Verma, S., Tomatis, V., Acharya, B. R., Balasubramaniam, L., Templin, R. M., McMahon, K.-A., Lee, Y. S., Ju, R. J., Stebhens, S. J., Ladoux, B., Mitchell, C. A., … Yap, A. S. (2020). Caveolae Control Contractile Tension for Epithelia to Eliminate Tumor Cells. Developmental Cell, 54(1), 75-91.e7. https://doi.org/10.1016/j.devcel.2020.05.002
Jain, S., Cachoux, V. M. L., Narayana, G. H. N. S., de Beco, S., D’Alessandro, J., Cellerin, V., Chen, T., Heuzé, M. L., Marcq, P., Mège, R.-M., Kabla, A. J., Lim, C. T., & Ladoux, B. (2020). The role of single cell mechanical behavior and polarity in driving collective cell migration. Nature Physics, 16(7), 802–809. https://doi.org/10.1038/s41567-020-0875-z
Doss, B. L., Pan, M., Gupta, M., Grenci, G., Mège, R.-M., Lim, C. T., Sheetz, M. P., Voituriez, R., & Ladoux, B. (2020). Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress. Proceedings of the National Academy of Sciences of the United States of America, 117(23), 12817–12825. https://doi.org/10.1073/pnas.1917555117
Beauchesne, C. C., Chabanon, M., Smaniotto, B., Ladoux, B., Goyeau, B., & David, B. (2020). Channeling Effect and Tissue Morphology in a Perfusion Bioreactor Imaged by X-Ray Microtomography. Tissue Engineering and Regenerative Medicine, 17(3), 301–311. https://doi.org/10.1007/s13770-020-00246-8
Khataee, H., Czirok, A., & Neufeld, Z. (2020). Multiscale modelling of motility wave propagation in cell migration. Scientific Reports, 10(1), 8128. https://doi.org/10.1038/s41598-020-63506-6
Coppin, L., Jannin, A., Ait Yahya, E., Thuillier, C., Villenet, C., Tardivel, M., Bongiovanni, A., Gaston, C., de Beco, S., Barois, N., van Seuningen, I., Durand, E., Bonnefond, A., Vienne, J.-C., Vamecq, J., Figeac, M., Vincent, A., Delacour, D., Porchet, N., & Pigny, P. (2020). Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface. Cell Death & Disease, 11(5), 360. https://doi.org/10.1038/s41419-020-2556-3
Thomas, M., Ladoux, B., & Toyama, Y. (2020). Desmosomal Junctions Govern Tissue Integrity and Actomyosin Contractility in Apoptotic Cell Extrusion. Current Biology: CB, 30(4), 682-690.e5. https://doi.org/10.1016/j.cub.2020.01.002
Hafsia, N., Forien, M., Renaudin, F., Delacour, D., Reboul, P., Van Lent, P., Cohen-Solal, M., Lioté, F., Poirier, F., & Ea, H. K. (2020). Galectin 3 Deficiency Alters Chondrocyte Primary Cilium Formation and Exacerbates Cartilage Destruction via Mitochondrial Apoptosis. International Journal of Molecular Sciences, 21(4), E1486. https://doi.org/10.3390/ijms21041486
Steinway, S. N., Saleh, J., Koo, B.-K., Delacour, D., & Kim, D.-H. (2020). Human Microphysiological Models of Intestinal Tissue and Gut Microbiome. Frontiers in Bioengineering and Biotechnology, 8, 725. https://doi.org/10.3389/fbioe.2020.00725
Bosch-Fortea, M., Rodriguez-Fraticelli, A. E., Herranz, G., Hachimi, M., Barea, M. D., Young, J., Ladoux, B., & Martin-Belmonte, F. (2019). Micropattern-based platform as a physiologically relevant model to study epithelial morphogenesis and nephrotoxicity. Biomaterials, 218, 119339. https://doi.org/10.1016/j.biomaterials.2019.119339
Heuzé, M. L., Sankara Narayana, G. H. N., D’Alessandro, J., Cellerin, V., Dang, T., Williams, D. S., Van Hest, J. C., Marcq, P., Mège, R.-M., & Ladoux, B. (2019). Myosin II isoforms play distinct roles in adherens junction biogenesis. ELife, 8, e46599. https://doi.org/10.7554/eLife.46599
Peyret, G., Mueller, R., d’Alessandro, J., Begnaud, S., Marcq, P., Mège, R.-M., Yeomans, J. M., Doostmohammadi, A., & Ladoux, B. (2019). Sustained Oscillations of Epithelial Cell Sheets. Biophysical Journal, 117(3), 464–478. https://doi.org/10.1016/j.bpj.2019.06.013
Nguyen, T., Duchesne, L., Sankara Narayana, G. H. N., Boggetto, N., Fernig, D. D., Uttamrao Murade, C., Ladoux, B., & Mège, R.-M. (2019). Enhanced cell-cell contact stability and decreased N-cadherin-mediated migration upon fibroblast growth factor receptor-N-cadherin cross talk. Oncogene, 38(35), 6283–6300. https://doi.org/10.1038/s41388-019-0875-6
Hu, S., Grobe, H., Guo, Z., Wang, Y.-H., Doss, B. L., Pan, M., Ladoux, B., Bershadsky, A. D., & Zaidel-Bar, R. (2019). Reciprocal regulation of actomyosin organization and contractility in nonmuscle cells by tropomyosins and alpha-actinins. Molecular Biology of the Cell, 30(16), 2025–2036. https://doi.org/10.1091/mbc.E19-02-0082
Yeo, M. S., Subhash, V. V., Suda, K., Balcıoğlu, H. E., Zhou, S., Thuya, W. L., Loh, X. Y., Jammula, S., Peethala, P. C., Tan, S. H., Xie, C., Wong, F. Y., Ladoux, B., Ito, Y., Yang, H., Goh, B. C., Wang, L., & Yong, W. P. (2019). FBXW5 Promotes Tumorigenesis and Metastasis in Gastric Cancer via Activation of the FAK-Src Signaling Pathway. Cancers, 11(6), E836. https://doi.org/10.3390/cancers11060836
Gauquelin, E., Tlili, S., Gay, C., Peyret, G., Mège, R.-M., Fardin, M. A., & Ladoux, B. (2019). Influence of proliferation on the motions of epithelial monolayers invading adherent strips. Soft Matter, 15(13), 2798–2810. https://doi.org/10.1039/c9sm00105k
Chen, T., Callan-Jones, A., Fedorov, E., Ravasio, A., Brugués, A., Ong, H. T., Toyama, Y., Low, B. C., Trepat, X., Shemesh, T., Voituriez, R., & Ladoux, B. (2019). Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nature Physics, 15, 393–402. https://doi.org/10.1038/s41567-018-0383-6
Gupta, M., Doss, B. L., Kocgozlu, L., Pan, M., Mège, R.-M., Callan-Jones, A., Voituriez, R., & Ladoux, B. (2019). Cell shape and substrate stiffness drive actin-based cell polarity. Physical Review. E, 99(1–1), 012412. https://doi.org/10.1103/PhysRevE.99.012412
Acharya, B. R., Nestor-Bergmann, A., Liang, X., Gupta, S., Duszyc, K., Gauquelin, E., Gomez, G. A., Budnar, S., Marcq, P., Jensen, O. E., Bryant, Z., & Yap, A. S. (2018). A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Developmental Cell, 47(4), 439-452.e6. https://doi.org/10.1016/j.devcel.2018.09.016
Nier, V., Peyret, G., d’Alessandro, J., Ishihara, S., Ladoux, B., & Marcq, P. (2018). Kalman Inversion Stress Microscopy. Biophysical Journal, 115(9), 1808–1816. https://doi.org/10.1016/j.bpj.2018.09.013


Ron, J. E., Joseph d’Alesandro, Cellerin, V., Voituriéz, R., Ladoux, B., & Gov, N. S. (2023). Polarization and motility of one-dimensional multi-cellular trains. Biophysical Journal. https://doi.org/10.1016/j.bpj.2023.11.003

Book chapters

Kuony, A., Brezak, M., Mège, R.-M., & Sumbalova Koledova, Z. (2024). Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland. In Z. Sumbalova Koledova (Ed.), 3D Cell Culture: Methods and Protocols (pp. 145–156). Springer US. https://doi.org/10.1007/978-1-0716-3674-9_10
Valon, L., & de Beco, S. (2021). Control of Cell Migration Using Optogenetics. Methods in Molecular Biology (Clifton, N.J.), 2179, 415–425. https://doi.org/10.1007/978-1-0716-0779-4_29
Xi, W., Sonam, S., Lim, C. T., & Ladoux, B. (2018). Tubular microscaffolds for studying collective cell migration. Methods in Cell Biology, 146, 3–21. https://doi.org/10.1016/bs.mcb.2018.05.001


Balasubramaniam, L., Mège, R.-M., & Ladoux, B. (2022). Active nematics across scales from cytoskeleton organization to tissue morphogenesis. Current Opinion in Genetics & Development, 73, 101897. https://doi.org/10.1016/j.gde.2021.101897
Doostmohammadi, A., & Ladoux, B. (2022). Physics of liquid crystals in cell biology. Trends in Cell Biology, 32(2), 140–150. https://doi.org/10.1016/j.tcb.2021.09.012
Saleh, J., Mercier, B., & Xi, W. (2021). Bioengineering methods for organoid systems. Biology of the Cell, 113(12), 475–491. https://doi.org/10.1111/boc.202000119
Xi, W., Delacour, D., & Ladoux, B. (2020). Designer substrates and devices for mechanobiology study. Journal of Semiconductors, 41(4), 041607. https://doi.org/10.1088/1674-4926/41/4/041607
Xi, W., Saw, T. B., Delacour, D., Lim, C. T., & Ladoux, B. (2019). Material approaches to active tissue mechanics. Nature Reviews Materials, 4(1), 23–44. https://doi.org/10.1038/s41578-018-0066-z
Chen, T., Saw, T. B., Mège, R.-M., & Ladoux, B. (2018). Mechanical forces in cell monolayers. Journal of Cell Science, 131(24), jcs218156. https://doi.org/10.1242/jcs.218156
Saw, T. B., Xi, W., Ladoux, B., & Lim, C. T. (2018). Biological Tissues as Active Nematic Liquid Crystals. Advanced Materials (Deerfield Beach, Fla.), 30(47), e1802579. https://doi.org/10.1002/adma.201802579
Ladoux, B., & Mège, R.-M. (2017). Mechanobiology of collective cell behaviours. Nature Reviews. Molecular Cell Biology, 18(12), 743–757. https://doi.org/10.1038/nrm.2017.98


Alexandre Kabla
Cambridge University, UK

Xavier Trepat
IBEC, Spain

Alpha Yap
University of Queensland, Australia

Julia Yeomans
Oxford University, UK

Michael Sheetz
Pakorn tony Kanchanawong
Lim chwee Teck
Yusuke Tonama
Yan Jie
Gianluca Grenci
Mechanobiology Institute (MBI), Singapore



Raphael Voituriez, Philippe Marcq
Sorbonne Université, Paris

Sylvie Hénon
Laboratoire Matière et Systèmes Complexes, Université de Paris

Philippe ChavrierChristophe LamazeJacques Prost
Institut Curie, Paris 

Olivier Goulet
Hôpital Necker-Enfants Malades, Paris

Yong Chen
Ecole Normale Supérieure, Département de Chimie, Paris

Bénédicte Dalaval
CRBM, Montpellier



Nicolas Borghi
Mechanotransduction: from Cell Surface to Nucleus

Nicolas Minc
Cellular Spatial Organization

Guillaume Romet-Lemonne & Antoine Jégou
Regulation of Actin Assembly Dynamics