Cell Adhesion and Mechanics

BENOIT LADOUX & RENÉ MARC MÈGE

Mechanical constraints and force transmission play an essential role in multicellular living organisms They are regulating basic biological processes such as morphogenesis, tumor metastasis and tissue repair. Cell adhesions, coupled to the contractile cytoskeleton, are major sites of force transmission in cells. This mechanical coupling which enables cells to sense, signal, and respond to physical changes in the environment, has however been largely understudied. In this context, we are studying the cooperation between adhesion, mechanical and biochemical signaling for the adaptation of living cells to changes in their physical environment at various scales, from single molecules to tissues.

Keywords: mechanobiology, mechanosensing, epithelia homeostasis, collective cell migration, microfabrication, biophysics, cell extrusion, tissue mechanics

+33 (0)157278071 / +33 (0)157278067 benoit.ladoux(at)ijm.fr / rene-marc.mege(at)ijm.fr @BLadoux @ReneMege

https://ladoux-mege-lab.cnrs.fr/

Research

AT THE SINGLE CELLS LEVEL

We study force sensing and mechanotransduction at integrin-mediated cell-extracellular matrix and cadherin-mediated cell-cell adhesions. To answer these questions, we have developed single cells and cell doublets models allowing a tight control of cell-matrix and cell-adhesion formation in a physically and mechanically defined microenvironment. Coupled with advanced microscopy, microforce sensor devices and classical cell biology, these approaches allow us to determine the molecular mechanisms that control cell adhesion and cytoskeleton remodeling, as well as cell shape and migration and their adaptation to environment compliance as well as to cytoskeleton visco-elastic properties and cell’s internal tension generated by myosin motors.

AT THE CELL ASSEMBLIES LEVEL

We study the collective behavior of cells in epithelial sheets, in the context of tissue homeostasis and wound healing. To answer these questions, we developing microfabricated tools and biophysical tools to measure and control the mechanical properties and topology of cell’s microenvironment. These tools are combined with molecular approaches, advanced techniques in light microscopy, image analysis and modeling to study the influence of physical properties of the environment on the organization of epithelial layers, collective cell migration, single and collective cell polarization, cell division and cell extrusion. We are characterizing how physical constraints can lead to emergent dynamical and mechanical properties of various epithelial tissues.

AT THE TISSUES AND ORGANOID LEVEL

We study how more complex epithelial tissues formed of mixed populations of cells (normal/adhesion deficient, normal/cancer, differentiation/stem cells) facing homogeneous and heterogeneous substrates (extracellular matrix chemistry, rigidity, geometry and topography), regulate homeostasis, segregate and/or auto assemble. Our aims are to determine i) how physical constraints of the microenvironment modulate mechanical properties of epithelial cells and tissues, ii) how they direct a variety of cell behaviors including stem cell proliferation, cell extrusion or delamination, cell migration, differentiation, and polarity, and iii) how they impact on the morphogenesis normal epithelial tissue, as well as the pathological development of intestinal rare diseases. Biomimetic substrates coupled to high resolution imaging and biochemistry are instrumental to reach these goals.

Members

Group Leaders:

Benoît LADOUX
Téléphone : +33 (0)157278071 / Email : benoit.ladoux (at) ijm.fr

René-Marc MÈGE
Téléphone : +33 (0)157278067 / Email : rene-marc.mege (at) ijm.fr

Membres de l’équipe :

ANGER	Lucas	Engineer
D’ALESSANDRO	Joseph	Researcher
DE BECO	Simon	teacher-researcher
DUBEY	Sushil	Postdoc
FARDIN	Marc-Antoine	Researcher
DANG	Tien	Engineer
PENETI	Sudheer Kumar	PhD Student
ROSSE	Carine	invited researcher
SCHONIT	Andreas	PhD student
SHEN	Yuan	Postdoc
WODRASCKA	Fanny	PhD Student
WU	Huiqiong	Postdoc
XI	Wang	Researcher
CHILUPURI	Ranjith Kumar	PhD Student
JIANG	Pan	Postdoctorant
MARTINS	Joana	PhD Student visitor
VIGNES	Hélène	Postdoc
GRUDTSYNA	Valeriia	PhD Student visitor
THIANT	Clémence	PhD Student
ARKOWITZ	Grégory	PhD Student
DAI	Wufei	PhD Student
KAILASAM MANI	Satish	Postdoc

Selected Publications

Balasubramaniam L, Doostmohammadi A, Saw TB, Narayana GHNS, Mueller R, Dang T, Thomas M, Gupta S, Sonam S, Yap AS, Toyama Y, Mège RM, Yeomans JM, Ladoux B. Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nat Mater. 2021 Aug;20(8):1156-1166. doi: 10.1038/s41563-021-00919-2. Epub 2021 Feb 18. Erratum in: Nat Mater. 2021 Mar 9;: PMID: 33603188; PMCID: PMC7611436.

Gaston C, De Beco S, Doss B, Pan M, Gauquelin E, D’Alessandro J, Lim CT, Ladoux B, Delacour D. EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nat Commun. 2021 Apr 13;12(1):2226. doi: 10.1038/s41467-021-22482-9. PMID: 33850145; PMCID: PMC8044225.

Jain S, Cachoux VML, Narayana GHNS, de Beco S, D’Alessandro J, Cellerin V, Chen T, Heuzé ML, Marcq P, Mège RM, Kabla AJ, Lim CT, Ladoux B. The role of single cell mechanical behavior and polarity in driving collective cell migration. Nat Phys. 2020 Jul;16(7):802-809. doi: 10.1038/s41567-020-0875-z. Epub 2020 May 4. PMID: 32641972; PMCID: PMC7343533.

Le AP, Rupprecht JF, Mège RM, Toyama Y, Lim CT, Ladoux B. Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nat Commun. 2021 Jan 15;12(1):397. doi: 10.1038/s41467-020-20563-9. PMID: 33452264; PMCID: PMC7810754.

Doss BL, Pan M, Gupta M, Grenci G, Mège RM, Lim CT, Sheetz MP, Voituriez R, Ladoux B. Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress. Proc Natl Acad Sci U S A. 2020 Jun 9;117(23):12817-12825.
doi: 10.1073/pnas.1917555117. Epub 2020 May 22. PMID: 32444491; PMCID: PMC7293595.

Heuzé ML, Sankara Narayana GHN, D’Alessandro J, Cellerin V, Dang T, Williams DS, Van Hest JC, Marcq P, Mège RM, Ladoux B. Myosin II isoforms play distinct roles in <i>adherens</i> junction biogenesis. Elife. 2019 Sep 5;8:e46599. doi: 10.7554/eLife.46599. PMID: 31486768; PMCID: PMC6756789.

Chen T, Callan-Jones A, Fedorov E, Ravasio A, Brugués A, Ong HT, Toyama Y, Low BC, Trepat X, Shemesh T, Voituriez R, Ladoux B. Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nat Phys. 2019 Apr;15:393-402. doi: 10.1038/s41567-018-0383-6. Epub 2019 Jan 21. PMID: 30984281; PMCID: PMC6456019.

Seddiki R, Narayana GHNS, Strale PO, Balcioglu HE, Peyret G, Yao M, Le AP, Teck Lim C, Yan J, Ladoux B, Mège RM. Force-dependent binding of vinculin to α-catenin regulates cell-cell contact stability and collective cell behavior.
Mol Biol Cell. 2018 Feb 15;29(4):380-388. doi: 10.1091/mbc.E17-04-0231. Epub 2017 Dec 27. PMID: 29282282; PMCID: PMC6014167.

Saw TB, Doostmohammadi A, Nier V, Kocgozlu L, Thampi S, Toyama Y, Marcq P, Lim CT, Yeomans JM, Ladoux B. Topological defects in epithelia govern cell death and extrusion. Nature. 2017 Apr 12;544(7649):212-216.
Abstract

Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, Mayeux A, Michaux G, Campeotto F, Lemale J, Viala J, Poirier F, Minc N, Schmitz J, Brousse N, Ladoux B, Goulet O, Delacour D. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017 Jan 13;8:13998.
Abstract

All the publications since 2017

Publications

Monteiro, P., Remy, D., Lemerle, E., Routet, F., Macé, A.-S., Guedj, C., Ladoux, B., Vassilopoulos, S., Lamaze, C., & Chavrier, P. (2023). A mechanosensitive caveolae–invadosome interplay drives matrix remodelling for cancer cell invasion. Nature Cell Biology, 1–17. https://doi.org/10.1038/s41556-023-01272-z

Eckert, J., Ladoux, B., Mège, R.-M., Giomi, L., & Schmidt, T. (2023). Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell density. Nature Communications, 14(1), 5762. https://doi.org/10.1038/s41467-023-41449-6

Lignieres, L., Sénécaut, N., Dang, T., Bellutti, L., Hamon, M., Terrier, S., Legros, V., Chevreux, G., Lelandais, G., Mège, R.-M., Dumont, J., & Camadro, J.-M. (2023). Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling. Journal of Proteome Research, 22(3), 996–1002. https://doi.org/10.1021/acs.jproteome.2c00699

Kawaue, T., Yow, I., Pan, Y., Le, A. P., Lou, Y., Loberas, M., Shagirov, M., Teng, X., Prost, J., Hiraiwa, T., Ladoux, B., & Toyama, Y. (2023). Inhomogeneous mechanotransduction defines the spatial pattern of apoptosis-induced compensatory proliferation. Developmental Cell, 58(4), 267-277.e5. https://doi.org/10.1016/j.devcel.2023.01.005

Rose, N., Estrada Chavez, B., Sonam, S., Nguyen, T., Grenci, G., Bigot, A., Muchir, A., Ladoux, B., Cadot, B., Le Grand, F., & Trichet, L. (2023). Bioengineering a miniaturized in vitro 3D myotube contraction monitoring chip to model muscular dystrophies. Biomaterials, 293, 121935. https://doi.org/10.1016/j.biomaterials.2022.121935

Sonam, S., Balasubramaniam, L., Lin, S.-Z., Ivan, Y. M. Y., Jaumà, I. P., Jebane, C., Karnat, M., Toyama, Y., Marcq, P., Prost, J., Mège, R.-M., Rupprecht, J.-F., & Ladoux, B. (2023). Mechanical stress driven by rigidity sensing governs epithelial stability. Nature Physics, 19, 132–141. https://doi.org/10.1038/s41567-022-01826-2

Gautier, B., Meneux, L., Feret, N., Audrain, C., Hudecek, L., Kuony, A., Bourdon, A., Le Guiner, C., Blouin, V., Delettre, C., & Michon, F. (2022). AAV2/9-mediated gene transfer into murine lacrimal gland leads to a long-term targeted tear film modification. Molecular Therapy. Methods & Clinical Development, 27, 1–16. https://doi.org/10.1016/j.omtm.2022.08.006

Kabla, A., Ladoux, B., & Di Meglio, J.-M. (2022). Tissue mechanics. The European Physical Journal. E, Soft Matter, 45(11), 90. https://doi.org/10.1140/epje/s10189-022-00240-z

d’Alessandro, J., Barbier-Chebbah, A., Voituriez, R., & Ladoux, B. (2022). [Cells as « Tom Thumbs » of living tissues]. Medecine Sciences: M/S, 38(11), 861–863. https://doi.org/10.1051/medsci/2022138

Petracchini, S., Hamaoui, D., Doye, A., Asnacios, A., Fage, F., Vitiello, E., Balland, M., Janel, S., Lafont, F., Gupta, M., Ladoux, B., Gilleron, J., Maia, T. M., Impens, F., Gagnoux-Palacios, L., Daugaard, M., Sorensen, P. H., Lemichez, E., & Mettouchi, A. (2022). Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction to control bacterial invasion and cell division. Nature Communications, 13(1), 6059. https://doi.org/10.1038/s41467-022-33803-x

Glentis, A., Blanch-Mercader, C., Balasubramaniam, L., Saw, T. B., d’Alessandro, J., Janel, S., Douanier, A., Delaval, B., Lafont, F., Lim, C. T., Delacour, D., Prost, J., Xi, W., & Ladoux, B. (2022). The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces. Science Advances, 8(37), eabn5406. https://doi.org/10.1126/sciadv.abn5406

Willems, M., Wells, C. F., Coubes, C., Pequignot, M., Kuony, A., & Michon, F. (2022). Hypolacrimia and Alacrimia as Diagnostic Features for Genetic or Congenital Conditions. Investigative Ophthalmology & Visual Science, 63(9), 3. https://doi.org/10.1167/iovs.63.9.3

Fardin, M. A., Hautefeuille, M., & Sharma, V. (2022). Spreading, pinching, and coalescence: the Ohnesorge units. Soft Matter, 18(17), 3291–3303. https://doi.org/10.1039/d2sm00069e

Liu, X., Tsubota, K., Yu, Y., Xi, W., & Gong, X. (2022). A numerical method to predict the membrane tension distribution of spreading cells based on the reconstruction of focal adhesions. Science China Physics, Mechanics & Astronomy, 65(6), 264612. https://doi.org/10.1007/s11433-021-1873-1

Xi, W., Saleh, J., Yamada, A., Tomba, C., Mercier, B., Janel, S., Dang, T., Soleilhac, M., Djemat, A., Wu, H., Romagnolo, B., Lafont, F., Mège, R.-M., Chen, Y., & Delacour, D. (2022). Modulation of designer biomimetic matrices for optimized differentiated intestinal epithelial cultures. Biomaterials, 282, 121380. https://doi.org/10.1016/j.biomaterials.2022.121380

Yang, Y.-A., Nguyen, E., Sankara Narayana, G. H. N., Heuzé, M., Fu, C., Yu, H., Mège, R.-M., Ladoux, B., & Sheetz, M. P. (2022). Local contractions regulate E-cadherin rigidity sensing. Science Advances, 8(4), eabk0387. https://doi.org/10.1126/sciadv.abk0387

Zisis, T., Brückner, D. B., Brandstätter, T., Siow, W. X., d’Alessandro, J., Vollmar, A. M., Broedersz, C. P., & Zahler, S. (2022). Disentangling cadherin-mediated cell-cell interactions in collective cancer cell migration. Biophysical Journal, 121(1), 44–60. https://doi.org/10.1016/j.bpj.2021.12.006

Sonam, S., Vigouroux, C., Jégou, A., Romet-Lemonne, G., Le Clainche, C., Ladoux, B., & Mège, R. M. (2021). Direct measurement of near-nano-Newton forces developed by self-organizing actomyosin fibers bound α-catenin. Biology of the Cell, 113(11), 441–449. https://doi.org/10.1111/boc.202100014

Jain, S., Ladoux, B., & Mège, R.-M. (2021). Mechanical plasticity in collective cell migration. Current Opinion in Cell Biology, 72, 54–62. https://doi.org/10.1016/j.ceb.2021.04.006

Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mège, R.-M., Yeomans, J. M., & Ladoux, B. (2021). Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1156–1166. https://doi.org/10.1038/s41563-021-00919-2

Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mège, R.-M., Yeomans, J. M., & Ladoux, B. (2021). Author Correction: Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1167. https://doi.org/10.1038/s41563-021-00974-9

d’Alessandro, J., Barbier-Chebbah, A., Cellerin, V., Benichou, O., Mège, R. M., Voituriez, R., & Ladoux, B. (2021). Cell migration guided by long-lived spatial memory. Nature Communications, 12(1), 4118. https://doi.org/10.1038/s41467-021-24249-8

Salvi, A. M., Bays, J. L., Mackin, S. R., Mege, R.-M., & DeMali, K. A. (2021). Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake. Nature Cell Biology, 23(5), 457–466. https://doi.org/10.1038/s41556-021-00677-y

Gaston, C., De Beco, S., Doss, B., Pan, M., Gauquelin, E., D’Alessandro, J., Lim, C. T., Ladoux, B., & Delacour, D. (2021). EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nature Communications, 12(1), 2226. https://doi.org/10.1038/s41467-021-22482-9

Latorre, E., Kale, S., Casares, L., Gómez-González, M., Uroz, M., Valon, L., Nair, R. V., Garreta, E., Montserrat, N., Del Campo, A., Ladoux, B., Arroyo, M., & Trepat, X. (2021). Addendum: Active superelasticity in three-dimensional epithelia of controlled shape. Nature, 592(7856), E30. https://doi.org/10.1038/s41586-021-03281-0

Le, A. P., Rupprecht, J.-F., Mège, R.-M., Toyama, Y., Lim, C. T., & Ladoux, B. (2021). Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nature Communications, 12(1), 397. https://doi.org/10.1038/s41467-020-20563-9

Ghisleni, A., Galli, C., Monzo, P., Ascione, F., Fardin, M.-A., Scita, G., Li, Q., Maiuri, P., & Gauthier, N. C. (2020). Complementary mesoscale dynamics of spectrin and acto-myosin shape membrane territories during mechanoresponse. Nature Communications, 11(1), 5108. https://doi.org/10.1038/s41467-020-18825-7

Tlili, S., Durande, M., Gay, C., Ladoux, B., Graner, F., & Delanoë-Ayari, H. (2020). Migrating Epithelial Monolayer Flows Like a Maxwell Viscoelastic Liquid. Physical Review Letters, 125(8), 088102. https://doi.org/10.1103/PhysRevLett.125.088102

Teo, J. L., Gomez, G. A., Weeratunga, S., Davies, E. M., Noordstra, I., Budnar, S., Katsuno-Kambe, H., McGrath, M. J., Verma, S., Tomatis, V., Acharya, B. R., Balasubramaniam, L., Templin, R. M., McMahon, K.-A., Lee, Y. S., Ju, R. J., Stebhens, S. J., Ladoux, B., Mitchell, C. A., … Yap, A. S. (2020). Caveolae Control Contractile Tension for Epithelia to Eliminate Tumor Cells. Developmental Cell, 54(1), 75-91.e7. https://doi.org/10.1016/j.devcel.2020.05.002

Jain, S., Cachoux, V. M. L., Narayana, G. H. N. S., de Beco, S., D’Alessandro, J., Cellerin, V., Chen, T., Heuzé, M. L., Marcq, P., Mège, R.-M., Kabla, A. J., Lim, C. T., & Ladoux, B. (2020). The role of single cell mechanical behavior and polarity in driving collective cell migration. Nature Physics, 16(7), 802–809. https://doi.org/10.1038/s41567-020-0875-z

Doss, B. L., Pan, M., Gupta, M., Grenci, G., Mège, R.-M., Lim, C. T., Sheetz, M. P., Voituriez, R., & Ladoux, B. (2020). Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress. Proceedings of the National Academy of Sciences of the United States of America, 117(23), 12817–12825. https://doi.org/10.1073/pnas.1917555117

Beauchesne, C. C., Chabanon, M., Smaniotto, B., Ladoux, B., Goyeau, B., & David, B. (2020). Channeling Effect and Tissue Morphology in a Perfusion Bioreactor Imaged by X-Ray Microtomography. Tissue Engineering and Regenerative Medicine, 17(3), 301–311. https://doi.org/10.1007/s13770-020-00246-8

Khataee, H., Czirok, A., & Neufeld, Z. (2020). Multiscale modelling of motility wave propagation in cell migration. Scientific Reports, 10(1), 8128. https://doi.org/10.1038/s41598-020-63506-6

Coppin, L., Jannin, A., Ait Yahya, E., Thuillier, C., Villenet, C., Tardivel, M., Bongiovanni, A., Gaston, C., de Beco, S., Barois, N., van Seuningen, I., Durand, E., Bonnefond, A., Vienne, J.-C., Vamecq, J., Figeac, M., Vincent, A., Delacour, D., Porchet, N., & Pigny, P. (2020). Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface. Cell Death & Disease, 11(5), 360. https://doi.org/10.1038/s41419-020-2556-3

Thomas, M., Ladoux, B., & Toyama, Y. (2020). Desmosomal Junctions Govern Tissue Integrity and Actomyosin Contractility in Apoptotic Cell Extrusion. Current Biology: CB, 30(4), 682-690.e5. https://doi.org/10.1016/j.cub.2020.01.002

Hafsia, N., Forien, M., Renaudin, F., Delacour, D., Reboul, P., Van Lent, P., Cohen-Solal, M., Lioté, F., Poirier, F., & Ea, H. K. (2020). Galectin 3 Deficiency Alters Chondrocyte Primary Cilium Formation and Exacerbates Cartilage Destruction via Mitochondrial Apoptosis. International Journal of Molecular Sciences, 21(4), E1486. https://doi.org/10.3390/ijms21041486

Steinway, S. N., Saleh, J., Koo, B.-K., Delacour, D., & Kim, D.-H. (2020). Human Microphysiological Models of Intestinal Tissue and Gut Microbiome. Frontiers in Bioengineering and Biotechnology, 8, 725. https://doi.org/10.3389/fbioe.2020.00725

Bosch-Fortea, M., Rodriguez-Fraticelli, A. E., Herranz, G., Hachimi, M., Barea, M. D., Young, J., Ladoux, B., & Martin-Belmonte, F. (2019). Micropattern-based platform as a physiologically relevant model to study epithelial morphogenesis and nephrotoxicity. Biomaterials, 218, 119339. https://doi.org/10.1016/j.biomaterials.2019.119339

Heuzé, M. L., Sankara Narayana, G. H. N., D’Alessandro, J., Cellerin, V., Dang, T., Williams, D. S., Van Hest, J. C., Marcq, P., Mège, R.-M., & Ladoux, B. (2019). Myosin II isoforms play distinct roles in adherens junction biogenesis. ELife, 8, e46599. https://doi.org/10.7554/eLife.46599

Peyret, G., Mueller, R., d’Alessandro, J., Begnaud, S., Marcq, P., Mège, R.-M., Yeomans, J. M., Doostmohammadi, A., & Ladoux, B. (2019). Sustained Oscillations of Epithelial Cell Sheets. Biophysical Journal, 117(3), 464–478. https://doi.org/10.1016/j.bpj.2019.06.013

Nguyen, T., Duchesne, L., Sankara Narayana, G. H. N., Boggetto, N., Fernig, D. D., Uttamrao Murade, C., Ladoux, B., & Mège, R.-M. (2019). Enhanced cell-cell contact stability and decreased N-cadherin-mediated migration upon fibroblast growth factor receptor-N-cadherin cross talk. Oncogene, 38(35), 6283–6300. https://doi.org/10.1038/s41388-019-0875-6

Hu, S., Grobe, H., Guo, Z., Wang, Y.-H., Doss, B. L., Pan, M., Ladoux, B., Bershadsky, A. D., & Zaidel-Bar, R. (2019). Reciprocal regulation of actomyosin organization and contractility in nonmuscle cells by tropomyosins and alpha-actinins. Molecular Biology of the Cell, 30(16), 2025–2036. https://doi.org/10.1091/mbc.E19-02-0082

Yeo, M. S., Subhash, V. V., Suda, K., Balcıoğlu, H. E., Zhou, S., Thuya, W. L., Loh, X. Y., Jammula, S., Peethala, P. C., Tan, S. H., Xie, C., Wong, F. Y., Ladoux, B., Ito, Y., Yang, H., Goh, B. C., Wang, L., & Yong, W. P. (2019). FBXW5 Promotes Tumorigenesis and Metastasis in Gastric Cancer via Activation of the FAK-Src Signaling Pathway. Cancers, 11(6), E836. https://doi.org/10.3390/cancers11060836

Gauquelin, E., Tlili, S., Gay, C., Peyret, G., Mège, R.-M., Fardin, M. A., & Ladoux, B. (2019). Influence of proliferation on the motions of epithelial monolayers invading adherent strips. Soft Matter, 15(13), 2798–2810. https://doi.org/10.1039/c9sm00105k

Chen, T., Callan-Jones, A., Fedorov, E., Ravasio, A., Brugués, A., Ong, H. T., Toyama, Y., Low, B. C., Trepat, X., Shemesh, T., Voituriez, R., & Ladoux, B. (2019). Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nature Physics, 15, 393–402. https://doi.org/10.1038/s41567-018-0383-6

Gupta, M., Doss, B. L., Kocgozlu, L., Pan, M., Mège, R.-M., Callan-Jones, A., Voituriez, R., & Ladoux, B. (2019). Cell shape and substrate stiffness drive actin-based cell polarity. Physical Review. E, 99(1–1), 012412. https://doi.org/10.1103/PhysRevE.99.012412

Acharya, B. R., Nestor-Bergmann, A., Liang, X., Gupta, S., Duszyc, K., Gauquelin, E., Gomez, G. A., Budnar, S., Marcq, P., Jensen, O. E., Bryant, Z., & Yap, A. S. (2018). A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Developmental Cell, 47(4), 439-452.e6. https://doi.org/10.1016/j.devcel.2018.09.016

Nier, V., Peyret, G., d’Alessandro, J., Ishihara, S., Ladoux, B., & Marcq, P. (2018). Kalman Inversion Stress Microscopy. Biophysical Journal, 115(9), 1808–1816. https://doi.org/10.1016/j.bpj.2018.09.013

Mège, R. M., & Ladoux, B. (2018). [The irruption of mechanics in the chemistry of life]. Medecine Sciences: M/S, 34(11), 963–971. https://doi.org/10.1051/medsci/2018241

Latorre, E., Kale, S., Casares, L., Gómez-González, M., Uroz, M., Valon, L., Nair, R. V., Garreta, E., Montserrat, N., Del Campo, A., Ladoux, B., Arroyo, M., & Trepat, X. (2018). Active superelasticity in three-dimensional epithelia of controlled shape. Nature, 563(7730), 203–208. https://doi.org/10.1038/s41586-018-0671-4

Preprint

Book chapters

Reviews

Theses

Collaborations

INTERNATIONAL

Alexandre Kabla
Cambridge University, UK

Xavier Trepat
IBEC, Spain

Alpha Yap
University of Queensland, Australia

Julia Yeomans
Oxford University, UK

Michael Sheetz
Pakorn tony Kanchanawong
Lim chwee Teck
Yusuke Tonama
Yan Jie
Gianluca Grenci
Mechanobiology Institute (MBI), Singapore

NATIONAL:

FRANCE

Raphael Voituriez, Philippe Marcq
Sorbonne Université, Paris

Sylvie Hénon
Laboratoire Matière et Systèmes Complexes, Université de Paris

Philippe Chavrier, Christophe Lamaze, Jacques Prost
Institut Curie, Paris

Olivier Goulet
Hôpital Necker-Enfants Malades, Paris

Yong Chen
Ecole Normale Supérieure, Département de Chimie, Paris

Bénédicte Dalaval
CRBM, Montpellier

INTERNAL

Nicolas Borghi
Mechanotransduction: from Cell Surface to Nucleus

Nicolas Minc
Cellular Spatial Organization

Guillaume Romet-Lemonne & Antoine Jégou
Regulation of Actin Assembly Dynamics

17/02/2022 – Post-doc

01/12/2021 – Post-doc -Dead or Alive project (ERC)