Post-doctoral Position – Localized mRNA translation at nuclear pore complexes
We are looking for a highly-motivated Post-doctoral Research Scientist to join the “RNA biogenesis and genome homeostasis” team led by Benoit Palancade at Institut Jacques Monod (Paris, France). Current work in the lab is aimed at discovering cis- and trans-acting regulations acting on mRNA metabolism, deciphering their impact on gene expression and genome integrity, and gaining insight into their spatial organization. In this context, we recently reported that a subset of mRNAs encoding nuclear proteins and nucleoporins are translated at nuclear pore complexes (NPCs) in budding yeast (Lautier et al., 2021).
The proposed project will further explore the molecular mechanisms underlying mRNA translation at NPCs, and determine the impact of localized protein synthesis on NPC assembly and other nuclear processes. For this purpose, the Post-doctoral fellow will combine transcriptome-wide strategies with mechanistic studies based on yeast imaging and molecular biology/biochemistry approaches. They will benefit from a highly dynamic and inclusive scientific environment, including a network of national and international collaborations, and will have access to the leading-edge core facilities available at IJM (including proteomics and imaging).
The applicant must hold a PhD in life sciences and have demonstrable experience in molecular biology and biochemistry. Prior expertise in mRNA biology, yeast models or bioinformatic analyses of transcriptomic data will be highly appreciated. Strong organizational and team working abilities, as well as verbal and written communication skills in English, will be required (French is not mandatory). The CV and publication record of the applicant should be competitive for national/international post-doctoral fellowships.
The project is supported by the International Collaborative Program from French Agence Nationale de la Recherche (joint grant with Prof. Martine Collart’s team, University of Geneva). The position is fixed-term for 2 years with the possibility of an extension, and may start at any time after December 1st 2023. Applications including a CV and a cover letter summarizing current and future research interests, in particular in relation to the proposed project, as well as the contact details of 2 referees, should be sent by e-mail to email@example.com.
Selection of publications from the host lab
Penzo A, Dubarry M, Brocas C, Zheng M, Mangione RM, Rougemaille M, Goncalves C, Lautier O, Libri D, Simon MN, Géli V, Dubrana K & Palancade B.(2023)A R-loop sensing pathway mediates the relocation of transcribed genes to nuclear pore complexes. Nature communications 14(1):5606
Hashimoto H, Ramirez DH, Lautier O, Pawlak N, Blobel G, Palancade B*, Debler EW*. (2022) Structure of the pre-mRNA leakage 39-kDa protein reveals a single domain of integrated zf-C3HC and Rsm1 modules. Scientific Reports 12(1):17691
Lautier O, Penzo A, Rouvière JO, Chevreux G, Collet L, Loïodice I, Taddei A, Devaux F, Collart MA & Palancade B. (2021) Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis. Molecular Cell 2021 81(11):2417-2427
Rouviere JO, Bulfoni M, Tuck A, Cosson B, Devaux F & Palancade B. (2018) A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits. Nature communications 9(1):1665
Bonnet A, Grosso AR, Elkaoutari A, Coleno E, Presle A, Sridhara SC, Janbon G, Géli V, de Almeida SF & Palancade B. (2017).
Introns protect eukaryotic genomes from transcription-associated genetic instability. Molecular Cell 67(4):608-621.e6