The Raote Lab contributes to the publication of a new article in Nature communications:
TUG protein acts through a disordered region to organize the early secretory pathway
Abstract:
The Endoplasmic Reticulum (ER)-Golgi Intermediate Compartment (ERGIC) is a network of tubules and vesicles known for producing COPI vesicles and receiving COPII vesicles from the ER. Much about its identity, stability, and regulation remains unknown. Here, we show that TUG (UBXN9, Aspscr1) protein, a central regulator of GLUT4 trafficking, localizes to the ERGIC, and that its deletion enhances anterograde flux of a model soluble cargo protein. TUG deletion redistributes ERGIC markers to the cis-Golgi and alters Golgi morphology. TUG forms biomolecular condensates in vitro and contains a central disordered region that mediates its recruitment to ERGIC membranes. A distinct N-terminal region mediates its oligomerization in cells. TUG deletion disrupts ERGIC-dependent processes, including autophagy and collagen secretion, and alters the targeting of the CFTR chloride channel. We conclude that TUG organizes and stabilizes ERGIC membranes to support their roles in diverse secretory and degradative membrane trafficking pathways.
Parchure A, Tejada H, Xi Z, Kim Y, Su M, Yan Y, Julca-Zevallos O, Alcázar-Román AR, Villemeur M, Liu X, Toomre D, Raote I, Bogan JS. TUG protein acts through a disordered region to organize the early secretory pathway. Nat Commun. 2025 Jul 1;16(1):5518. doi: 10.1038/s41467-025-60691-8. PMID: 40593538; PMCID: PMC12218103.
Figure © Parchure A et al.