Spinning Disk CSU-X1 FRAP

The Basic Principle of FRAP

Capabilities

High-speed multidimensional imaging with optical sectioning
Spinning disk modality produces high SNR images with minimal photobleaching
Inverted microscope
High sensitivity sCMOS cameras
Laser lines for all classes of fluorescent proteins
Incubation for mammalian cells
IR reflection based autofocus
BF and Nomarski contrast (DIC)
Dual channel
FRAP experiment
UV ablation experiment

Detailed Specifications

Microscope:

Axio Observer Z.1 Fully motorized Inverted stand
ASI Motorized stage with linear encoding
150 micron piezo for rapid z-control
Definit Focus 2 autofocus

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Life Imaging Services)

Scanhead:

Yokogawa CSU-X1 5000 rpm
dichroics – (405/488/561/640) (488/561) (488)

Lasers:

405 nm (100mw), 445 nm (80mW), 488 nm (150mW), 561 nm (150mW), 639 nm (160mW) (Gataca Systems)

Filters:

450/60 – 483/25 – 510/23 – 535/45 – 550/25 – 590/35 – 670/40

Objectives:

10x/0,45 Plan-Apochromat Ph1 (420641-9910) 0.916 µm/pixel
25x/0.8 LD LCI Plan-Apochromat DIC Imm Korr (UV) (420852-9870) 0.366 µm/pixel
40x/1.3 Plan-Apochromat Oil DIC (420762-9800) 0.229 µm/pixel
40x/1.2 C-Apochromat W Korr (421767-9970) 0.229 µm/pixel
63x/1,4 Plan-APOCHROMAT Oil (420782-9900) 0.145 µm/pixel
100x/1,4 Plan-APOCHROMAT Oil (420790-9901) 0.091 µm/pixel
Field of view max in software 1131 x 833 pixel

Cameras:

Two Cameras Prime 95B (Photometrics) for simultaneous display of dual channel

Computer:

Z840 (HP)
Xeon E5-2623 3GHZ 3GHZ; 32Go RAM
Acquisition Software MetaMorph 7.10