Spinning Disk CSU-W1

User Manual

Capabilities

• High-speed multidimensional imaging with optical sectioning
• Spinning disk modality produces high SNR images with minimal photobleaching
• Inverted microscope
• High sensitivity sCMOS cameras
• Laser lines for all classes of fluorescent proteins
• Incubation for mammalian cells
• IR reflection based autofocus
• BF and Nomarski contrast (DIC)

Detailed Specifications

Microscope:

• DMI8 Fully motorized Inverted stand (Leica)
• ASI Motorized stage with linear encoding
• 300 micron piezo for rapid z-control
• Adaptive Focus Control

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Life Imaging Services)

Scanhead:

• Yokogawa CSU-W1 4000 rpm
• dichroics – (405/488/561/640) (488/561) (488)

Lasers:
405 nm (100mw), 445 nm (80mW), 488 nm (150mW), 561 nm (150mW), 639 nm (160mW) (Gataca Systems)

Filters:
450/60 – 483/25 – 510/23 – 535/45 – 550/25 – 590/35 – 670/40

Objectives:

• 10x/0.40 HC PL APO (506284)   0.707 µm/pixel
• 20x/0.75 HC PL APO IMM CORR CS2 (506343)   0.350 µm/pixel
• 40x/1.30 HC PL APO OIL CS2 (506358)   0.175 µm/pixel
• 63x/1.40 HC PL APO OIL CS2 (506350)   0.112 µm/pixel
• 63x/1,2 W HCX PL APO CORR (506131)   0.112 µm/pixel
• 100x/1.40 HC PL APO OIL CS2 (506372)   0.070 µm/pixel
• Field of view  max in software 2048 x 2048 pixels

Cameras:
Two Cameras ORCA-FLASH 4 V2+ (Hamamatsu) for simultaneous display of dual channel

Computer:
Dell Precision Tower 7910

Xeon E5 2630 V3 @2,40GHz 2,4GHz; 64Go RAM
Acquisition Software MetaMorph 7.10