Confocal LSM980 Airyscan2 FLIM
The Basic Principle of Airyscan
The Basic Principle of FLIM
The Basic Principle of FCS
Capabilities
- High sensitivity point scanning confocal imaging
- Spectral array GaAsP detectors
- AiryScan2 SR module with up to 1.5x resolution gain and high SNR
- AiryScan2 8Y module with up to 8x speed and high SNR
- Inverted microscope
- Incubation for live cell timelapse – temp and CO2 control
- Timelapse with Definite focus2
- Multi-position
- Tiles acquisition and stitching of images
- BF and Nomarski contrast (DIC)
- FLIM and FCS module from Picoquant
Detailed Specifications
Zeiss Axio Observer Z.1 stand with Definite Focus2
Motorized XY stage
Piezo Z stage
Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes
Full environmental chamber with heating, humidity and CO2 control (Pecon)
Lasers:
405 nm, 445 nm, 488 nm, 561nm, 639nm Diode laser
Objectives:
- 10x/0.30 Plan-Apo WD: 5.2mm Air (420642-9800)
- 25x/0.8 Plan-Apochromat Imm LD LCI Corr DIC (420852-9870)
- 40x/1.3 Plan-Apochromat Oil DIC (420762-9800)
- 63x/1.4 Plan-Apochromat Oil DIC (420782-9900)
Detectors:
- GaAsP high QE 32 channel spectral array with unmixing capability
- 2 standard alkali PMTs in positions for blue and far-red detection
- AiryScan detector module and AiryScan Fast 4Y-8Y capability
- Transmitted light detector
Workstation Z6 (HP)
CPU: Intel Xeon ES-2623 v3 3.0 GHz; Memory: 32GB
Acquisition software Zen 3.5
Direct network link to Zeiss offline workstation – designed for Airyscan processing
FLIM and FCS module for LSM (PicoQuant) with Symphotime Software