L'équipe Veitia a publié une nouvelle revue dans Trends in biochemical sciences : Rethinking transcription factor dynamics and transcription regulation in eukaryotes Résumé : Transcription factors (TFs) control gene expression by binding to specific DNA motifs in cis-regulatory elements. Cooperativity has been thought to ensure TF binding specificity. Recent research suggests that, at least in yeast, the role…

L'équipe Romet-Lemonne/Jégou a contribué à la publication d'un nouvel article dans Nature communications : Histidine 73 methylation coordinates β-actin plasticity in response to key environmental factors Résumé : The functional importance of the methylation of histidine 73 (H73) in actin remains unclear. Focusing on cytoplasmic β-actin, present in all mammalian cells, we use molecular dynamics simulations with a polarizable…

L'équipe Palancade a publié un nouvel article dans Nature Communications : DNA lesions can frequently precede DNA:RNA hybrid accumulation   Résumé : While DNA:RNA hybrids contribute to multiple genomic transactions, their unscheduled formation is a recognized source of DNA lesions. Here, through a suite of systematic screens, we rather observed that a wide range of yeast mutant situations primarily…

L'équipe Conduit a publié un nouvel article dans Current Biology : Asymmetric microtubule nucleation from Golgi stacks promotes opposite microtubule polarity in axons and dendrites   Résumé : The neuronal microtubule cytoskeleton is highly polarized, with most microtubules growing away from the soma in axons (plus-end-out), but many microtubules growing toward the soma in dendrites (minus-end- out). This…

L'équipe Palancade a publié un nouvel article dans Nucleic Acid Research : RNA exosome-driven RNA processing instructs the duration of the unfolded protein response Résumé : Upon stresses, cellular compartments initiate adaptive programs meant to restore homeostasis. Dedicated to the resolution of transient perturbations, these pathways are typically maintained at a basal level, activated upon stress, and…

L'équipe Pintard a publié un nouvel article dans The EMBO Journal : The MAST kinase KIN-4 carries out mitotic entry functions of Greatwall in C. elegans   Résumé : MAST-like, or Greatwall (Gwl), an atypical protein kinase related to the evolutionarily conserved MAST kinase family, is crucial for cell cycle control during mitotic entry. Mechanistically, Greatwall is activated by…

L'équipe Léon a publié un nouvel article dans Biology of the cell : Direct observation of fluorescent proteins in gels: A rapid, cost-efficient, and quantitative alternative to immunoblotting Résumé : Background Information The discovery of green fluorescent protein (GFP) and its derivatives has revolutionized cell biology. These fluorescent proteins (FPs) have enabled the real-time observation of protein…

L'équipe Wassmann a publié un nouveau preprint : Elimination of separase inhibition reveals absence of cohesin protection in oocyte metaphase II   Résumé : The meiotic segregation pattern to generate haploid gametes is mediated by step-wise cohesion removal by separase, first from chromosome arms in meiosis I, and then from the centromere region in meiosis II. In mammalian…

L'équipe Romet-Lemonne/Jégou a publié une nouvelle revue dans Annual review of Biophysics : Mechanics of Single Cytoskeletal Filaments   Résumé : The cytoskeleton comprises networks of different biopolymers, which serve various cellular functions. To accomplish these tasks, their mechanical properties are of particular importance. Understanding them requires detailed knowledge of the mechanical properties of the individual filaments that…

L'équipe Borghi a publié une revue dans Front. Cell Dev: Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties   Résumé : Intracellular trafficking supports all cell functions maintaining the exchange of material between membrane-bound organelles and the plasma membrane during endocytosis, cargo sorting, and exocytosis/secretion. Several proteins of the intracellular trafficking machinery are…