Widefield Zeiss

User manuel

Capababilities

• High-speed multidimensional imaging for thin samples with minimal photobleaching
• Inverted microscope
• Filters cubes for all classes of fluorescent proteins
• BF; Phase contrast; Interferential contrast DIC
• Incubation for mammalian cells
• Z-stacks
• Time lapse
• Multi-position
• Tiles acquisition and stitching of images

Detailed Specifications

Axio Observer Z.1 Fully motorized Inverted stand (Zeiss Microscopy)
Motorized stage

fluorescence Cool LED pE 400 White

Fluorescence cubes filters:

• Dapi (365 D395 445/50)
• CFP (436/25 D455 480/40)
• YFP (500/24 D520 542/27)
• GFP (472/30 D495 520/35)
• RFP (531/40 D562 593/40)
• Cy5 (640/30 D660 690/50)

Available objectives:

• 5x/0.16 NeoFl dry Ph1, WD: 18,5mm, ∞/0.17
• 10x/0.45 Plan-Apo dry Ph1, WD: 2mm, ∞/0.17
• 20x/0.4 LD Plan-NeoFl dry Ph2, WD: 7.9mm, ∞/0-1.5
• 20x/0.8 Plan-Apo dry, WD: 0.550mm, ∞/0.17
• 25x/0.8 LD LCI Plan-Apo imm Corr, WD: 0.570mm, ∞/0-0.17
• 40x/0.6 LD Plan-NeoFl dry, WD: 2.9mm, ∞/0-1.5
• 40x/0.6 LD Plan-NeoFl dry Ph2, WD: 2.9mm, ∞/0-1.5
• 40x/1.3 Plan-Apo Oil, WD: 0.21mm, ∞/0.17
• 63x/1.4 Plan-Apo Oil, WD: 0.19mm, ∞/0.17
• 100x/1.4 Plan-Apo Oil, WD: 0.17mm, ∞/0.17

Environmental chamber with heating control (Life Imaging Services)
stage top incubation with CO₂ and humidity control

Camera : sCMOS Orca Flash 4 LT plus; 2048 x 2048 pixels (Hamamatsu)

Workstation Z;2 Core I3 3,6Go (HP)

Intel Xeon 5620 2,4GHZ; 32 GB RAM
Acquisition software ZEN 2.3 (Blue Edition)