L’équipe Palancade et la plateforme ProtéoSeine ont contribué à la publication d’un nouvel article dans PLOS Genetics :
Cellular imbalance of specific RNA binding proteins associates with harmful R-loops
Abstract :
Understanding how the assembly of nascent mRNA into a ribonucleoprotein (mRNP) influences R-loop homeostasis is crucial for gaining insight into the cellular mechanisms that prevent genome instability. Here, we identify three RNA-binding proteins, Rie1, Rim4 and She2, whose expression levels are important to limit R-loop accumulation and, thus, to prevent DNA damage. Interestingly, Rim4 and She2 are overrepresented in CBP80-containing mRNPs formed in the absence of THO. In addition, we found that an excess of the RNA exosome component Dis3 impairs its function, promoting R-loops, particularly from non-coding RNAs, which cause genomic instability. Our results indicate that changes in the availability of different RBPs or RNAs, causes R-loop-mediated DNA damage in the cell. These results may help to understand the mechanism that promotes cancer, as several RBPs are overexpressed in different types of tumors.
Mérida-Cerro JA, Chevreux G, Palancade B, Rondón AG, Aguilera A. Cellular imbalance of specific RNA binding proteins associates with harmful R-loops. PLoS Genet. 2025 Jul 2;21(7):e1011491. doi: 10.1371/journal.pgen.1011491. Epub ahead of print. PMID: 40601718.
Figure : Mérida-Cerro JA et al.