TELIGHT LIVE CODIM
User Manual (to update)
The basic principle of Conical Diffraction Imaging (CODIM)
Capabilities
- 3-in-1 imaging modes: widefield, confocal and super-resolution
- Super-resolution confocal structured illumination
- High sensitivity point scanning confocal-like imaging
- Laser lines for all classes of fluorescent proteins
- High sensitivity sCMOS camera
- Z-stacks
- Incubation for live cell timelapse – temperature and CO2 control
- Inverted microscope
Detailed specifications
Leica DMI6000 stand
Motorized stage 160 x 110 mm
Environmental chamber with heating control (Life Imaging Services), stage top incubation with CO2 and humidity control.
CoolLED pE-4000 light source for widefield imaging (16 individual wavelength available from 365 nm to 770 nm – see pE4000)
Lasers:
Toptica MLE delivering the following wavelengths: 405 nm (50 mW), 488 nm (50 mW), 561 nm (50 mW) and 640 nm (50 mW).
Fluorescence filter cubes:
- DAPI (Omega Optical XF131: Ex= 387AF28, Dic= 410DRLP, Em= 450AF65)
- Texas Red (Omega Optical XF102-2: Ex= 560AF55, Dic= 595DRLP, Em= 645AF65)
- Multi LED (Chroma quad band ET-89402)
- GFP Emission filter Thorlabs FESH550
Available objectives: (more objectives on demand)
- 63x/1.4 Plan-Apo Oil, WD: 0.19mm, ∞/0.17
Camera : sCMOS Kinetix ; 3200 x 3200 pixels (Teledyne – see detailed specifications here)
Workstation: Antec P20C equipped with 13th Gen Intel Core i7-13700KF (3.40 GHz); 128 GB RAM, NVIDIA Titan V 4095MB.
Operating system: Windows 11 Professional 64-bit
Acquisition software Telight LiveCodim.