Widefield Leica DMI8

User Manual

Capababilities

• High-speed multidimensional imaging for thin samples with minimal photobleaching
• Inverted microscope
• Filters cubes for all classes of fluorescent proteins
• BF; Phase contrast; Interferential contrast DIC,
• Environmental control: temperature, CO₂, humidity

• IR reflection based autofocus

• Z-stacks
• Time lapse
• Multi-position 

Detailed Specifications

Microscope:

• DMI8 Fully motorized Inverted stand (Leica)
• ASI Motorized stage with linear encoding
• Adaptive Hardware Focus Control (AFC)
• Metal halide fluorescence source
• Fluorescence cubes filters:
  Dapi (350/50 D400 460/50)
  GFP (470/40 D495 525/50)
  RFP (560/40 D585 630/75)
  Cy5 LP (620/60 D660 655lp)

Available objectives:

• 10x/0.40 HC PL APO (506284)
• 20x/0.75 HC PL APO IMM CORR CS2 (506343)
• 40x/1.30 HC PL APO OIL CS2 (506358)
• 63x/1.40 HC PL APO OIL CS2 (506350)
• 63x/1,2 W HCX PL APO CORR (506131)
• 100x/1.40 HC PL APO OIL CS2 (506372)

Field of view  max 2048 x 2048 pixels

Environmental control:
Full chamber with heating control
Stage top incubation with CO₂ and humidity control (Life Imaging Services)

Camera:
sCMOS ORCA-FLASH 4 V2+ (Hamamatsu)

Computer:
Dell Precision Tower 7910
Xeon E5 2630 V3, 2×2,4 GHz, 64 Go RAM
Windows 10

Acquisition Software:
MetaMorph 7.10