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Sandra Touati 

 

Depuis 2019 :  Chargé de recherche at CNRS (CRCN, section 22), Katja Wassmann’s team, Institute Jacques Monod, Paris

2015 – 2019 : Postdoctoral Researcher, Chromosome Segregation Laboratory, Frank Uhlmann’s team, Francis Crick Institute, London, UK.

2011 – 2014 :  PhD Physiology, Physiopathology and Therapeutic (ED394), Université Pierre et Marie Curie (UPMC), Paris.

 

La méiose est une forme spécialisée de division cellulaire qui assure la formation des gamètes et la stabilité du génome. Elle repose sur une reprogrammation profonde des réseaux de phosphorylation par rapport à la mitose. Ces réseaux sont guidés par un “phosphocode” : une combinaison de sites de phosphorylation, de motifs de reconnaissance et d’interactions avec des protéines régulatrices. Nous cherchons à comprendre comment ce phosphocode se met en place et se réorganise au cours de la méiose, en utilisant le modèle Saccharomyces cerevisiae. Notre approche combine l’analyse mécanistique des kinases et phosphatases, la phosphoprotéomique à grande échelle et résolue dans le temps, et la modélisation structurelle. Nous nous concentrons sur des enzymes clés du cycle cellulaire, notamment la kinase Cdk, la kinase spécifique de la méiose Ime2, et la phosphatase PP2A, pour comprendre comment leur activité et leur spécificité sont réorientées pendant la méiose. Ce projet vise à fournir de nouvelles connaissances sur la régulation méiotique, avec des implications pour la fertilité, la stabilité du génome et l’évolution des réseaux de signalisation spécialisés.

 

2022 – 2026       ANR JCJC, 300 k€

2021 – 2023       Project ARC PJA1, 50 k€

2020 – 2021        Tremplin FSI – Sorbonne université, 15 k€

 

  • Alessandra Carbone (IBPS, LCQB, Paris, France)
  • Philipp Kaldis (Lund University, Sweden)
  • Simonetta Piatti (CRBM, Montpellier, France)
  • Piotr Sicinski (Dana Farber cancer Institute, Boston, USA)

 

PhD student : Irem Polat (2023 – 2026), Dunja Celebic (2020 – 2023)

6 Master, 2 licence/BTS

 

ORCID # 0000-0002-0007-2130

Underlined – PhD candidates and students supervised

2025      El Jailani S, Cladière D, Nikalayevich E, Touati SA, Chesnokova V, Melmed S, Buffin E, Wassmann K. Eliminating separase inhibition reveals absence of robust cohesin protection in oocyte metaphase II. The EMBO Journal, 44: 5187-5214. Open Access

2025     Cladière D, Wassmann K, Touati SA*.  » JESS Simple western  » – a new technology to assess protein abundance in mouse oocytes. Methods in Molecular Biology, 2946:175-192.

* Corresponding author

2024     Varela Salgado M, Adriaans IE, Touati SA, Ibanes S, Lai-Kee-Him J, Ancelin A, Cipelletti L, Picas L, Piatti S. Phosphorylation of the F-BAR protein Hof1 drives septin ring splitting in budding yeast. Nature Communication, 15(1):3383. Open Access

2024     Celebic D, Polat I, Legros V, Chevreux G, Wassmann K, Touati SA*. Qualitative rather than quantitative phosphoregulation shapes the end of meiosis I in budding yeast. The EMBO Journal, 43: 1325-1350. Open Access

* Corresponding author

2024     El Jailani S, Wassmann K, Touati SA*. Whole immunofluorescence staining to visualize cell cycle progression in mouse oocytes. Methods in Molecular Biology, 2740: 211-227.

* Corresponding author

2022     Nikalayevich E, El Jailani S, Dupré A, Cladière D, Gryaznova Y, Fosse C, Buffin E, Touati SA, Wassmann K. Aurora B/C-dependent phosphorylation promotes Rec8 cleavage in mammalian oocytes. Current Biology. S0960-9822(22)00436-5. Open Access

2021     Gryaznova Y, Keating L#, Touati SA#, Cladière D, El Yakoubi W, Buffin E, Wassmann K. Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II. The EMBO Journal. 40(7):e106797. Open Access

# Co-second

2020     Jones AW, Flynn HR, Uhlmann F, Snijders AP*, Touati SA*. Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling. STAR protocols. 1(1):100022. Open Access

* Corresponding author

2020     Keating L, Touati SA, Wassmann KW. A PP2A-B56-Centered View on Metaphase-to-Anaphase Transition in Mouse Oocyte Meiosis I. Cells. 9(2):390. Open Access

2019     Touati SA*, Hofbauer L, Jones AW, Snijders AP, Kelly G, Uhlmann F*. Cdc14 and PP2A phosphatases cooperate to shape phosphoproteome dynamics during mitotic exit. Cell Reports. 29(7):2105-2119. Open Access

* Corresponding author

2018     Touati SA* and Uhlmann F*. A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit. Microbial Cell. 5, 389-392. Open Access

* Corresponding author

2018     Touati SA, Kataria M, Jones AW, Snijders AP, Uhlmann F. Phosphoproteome dynamics during mitotic exit in budding yeast. The EMBO Journal. 37: e98745. Open Access

2017     Godefrey M, Touati SA, Kataria M, Jones A, Snijders AP, Uhlmann F. PP2ACdc55 phosphatase imposes ordered cell cycle phosphorylation by opposing threonine phosphorylation. Molecular Cell. 65;393-402 e393. Open Access

2017     El Yakoubi W, Buffin E, Cladière D, Gryaznova Y, Berenger I, Touati SA, Gomez.R, Suja JA, van Deursen JM, Wassmann K. Mps1 kinase-dependent Sgo2 centromere localisation mediates cohesin protection in mouse oocyte meiosis I. Nature Communication. 8,694. Open Access

2016     Touati SA and Wassmann K. How oocytes try to get it right: spindle checkpoint control in meiosis. Chromosoma. 125, 321-335.

2015     Touati SA, Buffin E, Cladière D, Hached K, Rachez C, van Deursen JM, Wassmann K. Mouse oocytes depend on BubR1 for proper chromosome segregation but not for prophase I arrest. Nature Communication. 6, 6946. Open Access

2013     Chambon JP, Touati SA, Berneau S, Cladière D, Hebras C, Groeme R, McDougall A, Wassmann K. The PP2A inhibitor I2PP2A is essential for sister chromatid segregation in oocyte meiosis II. Current Biology. 23, 485-490. Open Access

2012     Touati SA, Cladière D, Lister LM, Leontiou I, Chambon JP, Rattani A, Böttger F, Stemmann O, Nasmyth K, Herbert M, Wassmann K. Cyclin A2 is required for sister chromatid separation, but not Separase control, in mouse oocyte meiosis. Cell Reports. 2, 1077-1087. Open Access

2012     Cromer L, Heyman J, Touati S, Harashima H, Araou E, Girard C, Horlow C, Wassmann K, Schnittger A, De Veylder L, Mercier R. OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TA. Plos Genetics. 8, e1002865. Open Access

 

2025 – now        Member of the Institute Jacques Monod council, representative rang B

2024 – now        Member of the COPIL for the LID (Laboratory of Interdisciplinarity studies on the doctorate) of Adoc Talent Management

2020 – now        Secretaire generale and Member of the Executive Committee Association Nationale des docteurs (ANDès)