Paris Postdoc Seminar – Nike Walther

Invitée par l’Institut Jacques Monod, Nike Walther (Institut Curie) présentera le Paris Postdoc Seminar :

« Transcription factors in action during cell state transitions – quantitative insights from live-cell single-molecule imaging in intestinal organoid models »

Résumé :

Transcription factors in action during cell state transitions – quantitative insights from live-cell single-molecule imaging in intestinal organoid models
Transcription factors (TFs) mediate gene expression changes during embryonic development and adult differentiation. They thus represent one regulatory level crucial for the generation and maintenance of healthy tissues. However, it remains poorly understood how the biophysical properties and the dosage of TFs dynamically regulate cell state transitions. To address these questions in in vitro models of the rapidly renewing mammalian intestinal epithelium, I established live-cell single-molecule tracking (SMT) and proximity-assisted photoactivation (PAPA)-SMT for dynamic protein interaction studies in multicellular intestinal organoids, and I co-developed an automated SMT imaging and analysis pipeline to resolve cellular subpopulations in heterogenous differentiation models. In this talk I will present how these technological advances allowed me to determine lineage TF-mediated regulatory principles of intestinal differentiation based on context-dependent TF single-molecule dynamics. I will further outline the power of our automated live-cell SMT framework to generate testable hypotheses towards unraveling molecular mechanisms underlying tissue-level phenotypes.