Stellaris Sted

User Manual

Capabilities

• High sensitivity Confocal Laser Scanning imaging
• Super resolution 3D STED microscopy for fixed or living sample
• Inverted microscope
• Incubation for live cell timelapse – temp and CO₂ control

• Timelapse with IR reflection based autofocus
• Multi-position

• Tiles acquisition and stitching of images
• LIGHTNING adaptative deconvolution

Detailed Specifications

Leica DMi8 CS stand with AFC
Motorized Piezo stage 120 mm x 80 mm
Z-Piezo stage 500µm
Metal halide fluorescence source with triple band pass fluorescence cube for widefield eyepiece visualization.

Full environmental chamber with heating, humidity and CO₂ controls (OKOLab)

Lasers: 405 nm (50mW), Whilte Laser Plus 440 nm up to 790 nm; STED Laser 775 nm

Detectors: two HyD X SP and one HyD S,Transmitted light detector

Objectives:

• HC PL APO 10x/0,45 (506410)
• HC PL APO 20x/0,75 IMM CORR CS2 (506343)
• HC PL APO 40x/1,25 GLYC CORR CS2 (506422)
• HC PL APO 93x/1,30 GLYC motCORR STED (506417)
• HC PL APO 100x/1,40 OIL STED WHITE (506378)

Workstation Z6 G5 (HP)
Microscope software “LAS X”