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X-WR-CALNAME:Institut Jacques Monod
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X-WR-CALDESC:Évènements pour Institut Jacques Monod
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DTSTART;TZID=Europe/Paris:20240906T114500
DTEND;TZID=Europe/Paris:20240906T130000
DTSTAMP:20260509T153516
CREATED:20240814T140519Z
LAST-MODIFIED:20240821T115723Z
UID:22776-1725623100-1725627600@www.ijm.fr
SUMMARY:Séminaire de l'Institut Jacques Monod - Amir Aharoni
DESCRIPTION:Invité par l’équipe Palancade\, Amir Aharoni (Department of Life Sciences and the National Institute for Biotechnology in the Negev\, Ben-Gurion University of the Negev\, Israel) présentera un séminaire de l’Institut Jacques Monod sur le thème : \nReal-time monitoring of replication fork progression through damaged DNA \n  \nRésumé : \nDespite the frequent occurrence of AP sites in the eukaryotic genome\, understanding how replication forks bypass non-repaired AP sites remains elusive. While an error-prone and an error-free DNA damage bypass pathways exist\, the choice and timing of activation of each bypass pathway during AP sites replication are still unknown. Here\, we have established and examined a system for measuring replication fork progression through site-specific AP sites in live yeast cells\, for monitoring DNA damage bypass in real time. In this system\, AP sites are introduced site-specifically between two bacterial fluorescent repressor-operator systems (FROS) during the G1 phase\, using catalytically inactive Cas9 fused to DNA glycosidase. Time-lapse fluorescent microscopy is then used to monitor the progression of single replisomes through the AP sites and to reveal the timing of AP bypass. Using this real-time microscopy system\, we revealed the cellular choice between replication associated and post replicative AP sites bypass pathways controlling replication fork stalling at AP sites and cell survival.
URL:https://www.ijm.fr/event/seminaire-ijm-amir-aharoni/
LOCATION:Institut Jacques Monod Salle François Jacob\, 15 rue Hélène Brion\, Paris\, 75013\, France
CATEGORIES:Séminaire de l'Institut Jacques Monod
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