Spinning Disk CSU-X1 Zeiss

 

User Manual

Capabilities

• High-speed multidimensional imaging with optical sectioning
• Spinning disk modality produces high SNR images with minimal photobleaching
• Inverted microscope
• High sensitivity sCMOS cameras
• Laser lines for all classes of fluorescent proteins
• Incubation for mammalian cells
• Dual channel
• BF and Nomarski contrast (DIC)
• Tiles acquisition and stitching of images

Detailed specifications

Microscope:

Axio Observer Z.1 Fully motorized Inverted stand
Motorized XY stage

Colibri Led fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Life Imaging Services)

Scanhead:

• Yokogawa CSU-X1 5000 rpm
• dichroics – (405/488/561/640)

Lasers:

405 nm (50mw), 488 nm (50mW), 561 nm (75mW), 638 nm (75mW)

Emission Filters:

BP 450/50 – BP 525/50 – BP 630/98 – BP 690/50

Objectives:

• 10x/0,3 EC Plan-NEOFLUAR Ph1 (420341-9911)   0.650 µm/pixel
• 25x/0.8 LD LCI Plan-Apochromat Imm Corr DIC (420852-9871)   0.260 µm/pixel
• 40x/1.3 Plan-Apochromat Oil DIC (420762-9800)   0.163µm/pixel
• 63x/1.4 Plan-Apochromat Oil DIC (420782-9900)   0.103 µm/pixel
• 100x/1.46 a Plan-Apochromat Oil DIC (420792-9800-720)   0.065 µm/pixel
• Field of view  max in software 1024 x 1024 pixels

Cameras:

Two cameras Orca-Fusion Gen III (Hamamatsu) for simultaneous display of dual channel

Computer:

Z840 (HP)
Xeon E5-2623 V3 3GHZ 3GHZ; 128Go RAM
Acquisition Software ZEN blue 2.6