Spinning Disk CSU-X1 FRAP

 

User Manual

The Basic Principle of FRAP

Capabilities

• High-speed multidimensional imaging with optical sectioning
• Spinning disk modality produces high SNR images with minimal photobleaching
• Inverted microscope
• High sensitivity sCMOS cameras
• Laser lines for all classes of fluorescent proteins
• Incubation for mammalian cells
• IR reflection based autofocus
• BF and Nomarski contrast (DIC)
• Dual channel
• FRAP experiment
• UV ablation experiment

Detailed Specifications

Microscope:

Axio Observer Z.1 Fully motorized Inverted stand
ASI Motorized stage with linear encoding
150 micron piezo for rapid z-control
Definit Focus 2 autofocus

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Life Imaging Services)

Scanhead:

• Yokogawa CSU-X1 5000 rpm
• dichroics – (405/488/561/640) (488/561) (488)

Lasers:

405 nm (100mw), 445 nm (80mW), 488 nm (150mW), 561 nm (150mW), 639 nm (160mW) (Gataca Systems)

Filters:

450/60 – 483/25 – 510/23 – 535/45 – 550/25 – 590/35 – 670/40

Objectives:

• 10x/0,45 Plan-Apochromat Ph1 (420641-9910)   0.916 µm/pixel
• 25x/0.8 LD LCI Plan-Apochromat DIC Imm Korr (UV) (420852-9870)   0.366 µm/pixel
• 40x/1.3 Plan-Apochromat Oil DIC (420762-9800)     0.229 µm/pixel
• 40x/1.2 C-Apochromat W Korr (421767-9970)   0.229 µm/pixel
• 63x/1,4 Plan-APOCHROMAT Oil (420782-9900)   0.145 µm/pixel
• 100x/1,4 Plan-APOCHROMAT Oil  (420790-9901)   0.091 µm/pixel
• Field of view  max in software 1131 x 833 pixel

Cameras:

Two Cameras Prime 95B (Photometrics) for simultaneous display of dual channel

Computer:

Z840 (HP)
Xeon E5-2623 3GHZ 3GHZ; 32Go RAM
Acquisition Software MetaMorph 7.10