RNA biogenesis and genome homeostasis

 

 

Guaranteeing genome integrity while ensuring gene expression is a permanent challenge in living cells: while the regulated production of messenger RNAs is critical for the establishment of gene expression patterns, it can compete with the maintenance and the duplication of chromosomes. In this context, our team explores the mechanisms that keep in check the different stages of mRNA metabolism, from their synthesis in the nucleus to their export and translation in the cytoplasm. We are specially willing to understand:

– how mRNA biogenesis is synchronized with other DNA-dependent molecular transactions, by counteracting the formation of genotoxic DNA:mRNA hybrids (or R-loops);

– how these processes are spatially organized, in particular with respect to nuclear pores, the macromolecular complexes ensuring the transit of mRNAs out of the nucleus.

To address these questions, the integrative approach adopted by our team combines systematic and mechanistic approaches in a unicellular model eukaryote, the yeast S. cerevisiae .

 

Group Leader : Benoit Palancade

Research Director CNRS

+33 (0)157278039

benoit.palancade(at)ijm.fr

@PalancadeLab

ORCID: 0000-0002-3894-9735

 

 

RESEARCHMEMBERSPUBLICATIONSNEWSJOB

 

Research Topics:

 

Biologie quantitative et modélisation

Dynamique cellulaire et signalisation

Dynamique du génome et des chromosomes

Pathologies moléculaires et cellulaires

 

Selected publications:

 

Palancade, B & Rothstein, R (2021)

The ultimate (mis)-match: when DNA meets RNA

Cells 10(6):1433

https://www.mdpi.com/2073-4409/10/6/1433

 

Lautier O, Penzo A, Rouvière JO, Chevreux G, Collet L, Loïodice I, Taddei A, Devaux F, Collart MA, Palancade B. (2021)

Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis.

Mol Cell. 2021 Apr 4:S1097-2765(21)00225-2.

https://www.sciencedirect.com/science/article/abs/pii/S1097276521002252

Image de couverture: https://www.cell.com/molecular-cell/issue?pii=S1097-2765(20)X0012-8

Voir les actualités du CNRS (INSB) et de l’Université de Paris.

https://insb.cnrs.fr/fr/cnrsinfo/des-arn-messagers-traduits-aux-pores-nucleaires

https://u-paris.fr/des-proteines-cellulaires-fabriquees-a-la-surface-du-noyau/

 

Rouviere JO, Bulfoni M, Tuck A, Cosson B, Devaux F & Palancade B. (2018)

A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits.

Nature communications 9(1):1665

https://www.nature.com/articles/s41467-018-03673-3

 

Bonnet A, Grosso AR, Elkaoutari A, Coleno E, Presle A, Sridhara SC, Janbon G, Géli V, de Almeida SF & Palancade B. (2017)

Introns protect eukaryotic genomes from transcription-associated genetic instability

Mol Cell 67(4):608-621.e6

https://www.sciencedirect.com/science/article/pii/S1097276517304963?via%3Dihub

Voir le commentaire dans Science et l’actualité du CNRS(INSB):

http://science.sciencemag.org/content/357/6354/883.5

https://www.cnrs.fr/insb/recherche/parutions/articles2017/b-palancade.html

Cet article a été élu “article de l’année 2017” par la SFBBM (Société Française de Biochimie et de Biologie Moléculaire)

https://www.sfbbm.fr/index.php?option=com_content&view=featured&Itemid=364&lang=fr

 

Bonnet A, Bretes H & Palancade B.(2015)

Nuclear pores affect distinct stages of intron-containing gene expression

Nucleic Acids Research 43(8):4249-61

https://academic.oup.com/nar/article/43/8/4249/2414571

 

Bretes H, Rouviere JO, Léger T, Oeffinger M, Devaux F, Doye V and Palancade B. (2014)

Sumoylation of the THO complex regulates the biogenesis of a subset of mRNPs 

Nucleic Acids Research  42(8):5043-58

https://academic.oup.com/nar/article/42/8/5043/1069049

 

Bonnet A & Palancade B. (2014)

Regulation of mRNA trafficking by nuclear pore complexes 

Genes 5 :767-791 Special issue « Mechanisms of mRNA export »

http://www.mdpi.com/2073-4425/5/3/767/htm

 

Rouviere JO, Geoffroy MC and Palancade B. (2013)

Multiple cross talks between mRNP biogenesis and SUMO 

Chromosoma 122(5):387-99

https://link.springer.com/article/10.1007%2Fs00412-013-0408-y