Metabolism and Function of RNA in the Nucleus
We seek to understand the nuclear pathways that shape transcriptomes, thereby determining cellular functions. We are broadly interested in the molecular mechanisms and physiological functions of transcripional and post-transcriptional control of gene expression using genome-wide approaches, molecular genetics, and biochemistry. We also aim at undrestanding how perturbations in these processes are involved in diseases. We use budding yeast and human cells in culture as model systems.
Xie, J., Aiello, U., Clement, Y., Haidara, N., Girbig, M., Schmitzova, J., Pena, V., Müller, C. W. , Libri, D. and Porrua, O.
Haidara, N. and Porrua, O. Modulated termination of non-coding transcription partakes in the regulation of gene expression. doi: https://doi.org/10.1101/2021.01.06.425579.
Villa, T., Barucco, M., Martin-Niclos, M.-J., Jacquier, A., and Libri, D. (2020). Degradation of Non-coding RNAs Promotes Recycling of Termination Factors at Sites of Transcription. Cell Rep 32, 107942.
Appanah, R., Lones, E.C., Aiello, U., Libri, D., and De Piccoli, G. (2020). Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability. Cell Rep 30, 2094-2105.e9.
Han, Z., Jasnovidova, O., Haidara, N., Tudek, A., Kubicek, K., Libri, D., Stefl, R., and Porrua, O. (2020). Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1. EMBO J. e101548.
Porrua, O (2020). Purification and in vitro analysis of the exosome cofactors Nrd1-Nab3 and Trf4-Air2. Methods Mol Biol 2062:277-289.
Kubik, S., Bruzzone, M.J., Challal, D., Dreos, R., Mattarocci, S., Bucher, P., Libri, D., and Shore, D. (2019). Opposing chromatin remodelers control transcription initiation frequency and start site selection. Nat. Struct. Mol. Biol. 26, 744–754.
Wang, S., Han, Z., Libri, D., Porrua, O., and Strick, T.R. (2019). Single-molecule characterization of extrinsic transcription termination by Sen1 helicase. Nat Commun 10, 1545.
Candelli, T., Gros, J.*, and Libri, D*. (2018). Pervasive transcription fine-tunes replication origin activity. Elife 7.
Challal, D., Barucco, M., Kubik, S., Feuerbach, F., Candelli, T., Geoffroy, H., Benaksas, C., Shore, D., and Libri, D. (2018). General Regulatory Factors Control the Fidelity of Transcription by Restricting Non-coding and Ectopic Initiation. Mol. Cell 72, 955-969.e7.
Han, Z., Porrua, O. (2018) Helicases as transcription termination factors: Different solutions for a common problem. Transcription 9(3):152-158.
Candelli, T., Challal, D., Briand J-B, Boulay J., Porrua O., Colin J., and Libri D. (2018) High-resolution transcription maps reveal the widespread impact of roadblock termination in S. cerevisiae. EMBO J. 2018 Jan 19. pii: e97490