Membrane Trafficking, Ubiquitin and Signalling

 

 

Our team seeks to determine the adaptation strategies of cells to nutritional stresses, such as nutrient deficiencies or inhibition of metabolism. Our theme is at the intersection of cell biology, cell signaling and metabolism, using the yeast Saccharomyces cerevisiae as a model.

 

Group Leader: Sébastien Léon

Research Director CNRS

+33 (0)157278057

sebastien.leon(at)ijm.fr

@SebLeon5

https://orcid.org/0000-0002-2536-8595

 

 

RESEARCHMEMBERSPUBLICATIONS

 

Research Topics :

 

Theme : Cellular dynamics and signalling (Signalling, trafficking and membranes, ubiquitin, endocytosis-exocytosis)

Axes : Quantitative biology and modelling (cell imaging, proteomics, regulatory networks), Molecular and cellular pathologies (cancer)

Models : yeast, cell lines

 

Selected publications :

 

  1. Kahlhofer J, Leon S, Teis D, Schmidt O. The alpha-arrestin family of ubiquitin ligase adaptors links metabolism with selective endocytosis. Biology of the cell. 2021;113(4):183-219. Epub 2020/12/15. doi: 10.1111/boc.202000137. PubMed PMID: 33314196.
  2. Le Boulch M, Brossard A, Le Dez G, Leon S, Rabut G. Sensitive detection of protein ubiquitylation using a protein fragment complementation assay. J Cell Sci. 2020;133(12):jcs.240093. doi: 10.1242/jcs.240093. PubMed PMID: 32409563.
  3. Laussel C, Leon S. Cellular toxicity of the metabolic inhibitor 2-deoxyglucose and associated resistance mechanisms. Biochem Pharmacol. 2020;182:114213. doi: 10.1016/j.bcp.2020.114213. PubMed PMID: 32890467.
  4. Ivashov V, Zimmer J, Schwabl S, Kahlhofer J, Weys S, Gstir R, Jakschitz T, Kremser L, Bonn GK, Lindner H, Huber LA, Leon S, Schmidt O, Teis D. Complementary alpha-arrestin-ubiquitin ligase complexes control nutrient transporter endocytosis in response to amino acids. eLife. 2020;9. doi: 10.7554/eLife.58246. PubMed PMID: 32744498.
  5. Defenouillėre Q, Verraes A, Laussel C, Friedrich A, Schacherer J, Leon S. The induction of HAD-like phosphatases by multiple signaling pathways confers resistance to the metabolic inhibitor 2-deoxyglucose. Sci Signal. 2019;12(597):aaw8000. doi: 10.1126/scisignal.aaw8000. PubMed PMID: 31481524.
  6. Hovsepian J, Albanese V, Becuwe M, Ivashov V, Teis D, Leon S. The yeast arrestin-related protein Bul1 is a novel actor of glucose-induced endocytosis. Mol Biol Cell. 2018;29(9):1012-20. doi: 10.1091/mbc.E17-07-0466. PubMed PMID: 29514933; PMCID: PMC5921569.
  7. Hovsepian J, Defenouillere Q, Albanese V, Vachova L, Garcia C, Palkova Z, Leon S. Multilevel regulation of an alpha-arrestin by glucose depletion controls hexose transporter endocytosis. J Cell Biol. 2017;216(6):1811-31. Epub 2017/05/05. doi: 10.1083/jcb.201610094. PubMed PMID: 28468835; PMCID: PMC5461024.
  8. Becuwe M, Leon S. Integrated control of transporter endocytosis and recycling by the arrestin-related protein Rod1 and the ubiquitin ligase Rsp5. eLife. 2014;3:03307. doi: 10.7554/eLife.03307. PubMed PMID: 25380227; PMCID: 4244573.
  9. Becuwe M, Vieira N, Lara D, Gomes-Rezende J, Soares-Cunha C, Casal M, Haguenauer-Tsapis R, Vincent O, Paiva S, Léon S. A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis. J Cell Biol. 2012;196(2):247-59. Epub 2012/01/18. doi: 10.1083/jcb.201109113. PubMed PMID: 22249293.
  10. Leon S, Erpapazoglou Z, Haguenauer-Tsapis R. Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Mol Biol Cell. 2008;19(6):2379-88. Epub 2008/03/28. doi: 10.1091/mbc.E08-01-0068. PubMed PMID: 18367543.