Comparative Developmental Neurobiology

Nikos KONSTANTINIDES

In the lab of Comparative Developmental Neurobiology, our goal is to understand how the impressive diversity of neurons that can be found in the animal brain develops during the hours, days, or weeks of embryonic development and how it has evolved over the millions of years of evolution. We use single-cell sequencing and Drosophila powerful genetic tools to characterize complex neurodevelopmental mechanisms and understand how they have evolved.

Keywords :Neuro-evo-devo, Drosophila melanogaster, Neuronal development, Evolution of developmental mechanisms, Visual system, Single-cell sequencing

+33 (0)157278145     nikos.konstantinides(at)ijm.fr     @nkonst4    www.konstantinides-lab.com

THE QUESTION THAT DRIVES OUR RESEARCH

Neurons are the most diverse cell type in the animal body in terms of morphology, physiology, molecular identity, activity, function, etc. In the lab, we try to understand how this impressive neuronal diversity is being generated. We study this in two different timescales, first in the few hours, days, or months of embryonic development, and second over the millions of years of animal evolution.

In other words, we want to answer :

  • How does a neuron acquire a neuronal type identity and how does it implement it?
  • How do neurons that are tightly interconnected in circuits evolve to output new behaviours?

 

OUR MODEL

We are using the insect visual system as a model (Figure 1). Insects inhabit diverse environments with different visual landscapes and stimuli. While preserving the overall organisation of their visual system (optic lobes), they have adapted it to their environment. Thus, insect optic lobes represent an ideal system to address how neuronal systems evolve to support different behaviours. Indeed, over the last 10 years, the Drosophila optic lobe has emerged as an ideal system to address these questions for four reasons: a) It is an experimentally manageable, albeit complex structure, for which we have a very comprehensive catalogue of neuronal cell types. Meticulous work from 30 years ago had identified 100 cell types in the optic lobes based solely on morphological characters. Recent work took advantage of elaborate genetic tools, as well as single-cell mRNA sequencing, to expand the number of neuronal cell types to 169, based on both morphology and molecular identity (Figure 2). Importantly, many of the optic lobe cell types are present in multiple copies in the brain (ranging from 50 to 800), which makes clustering of single-cell sequencing (scSeq) data and cluster annotation easier. b) We know the basic principles by which most of these neuronal cell types are generated during development and how they are specified. The interplay of three mechanisms is responsible for the generation of the 169 different neuronal types: spatial patterning diversifies neural stem cells based on their spatial origin, temporal patterning allows neural stem cells of different ages to sequentially give birth to different cell types; while a Notch-driven binary cell fate decision made at the time of division of the precursor, results in the generation of two different neurons. c) A detailed connectomic map of the medulla (the largest optic ganglion), as well as a thorough enumeration of the synapses between different cell types can be used to probe circuitry. Recently, an expanded EM reconstruction of the whole brain has been made available to track neurons and circuits. d) Finally, the neuronal circuits responsible for different behaviours, such as motion detection and colour vision, walking, jumping, etc are relatively well characterized. Nonetheless, studies in one species alone cannot address evolutionary questions; the Drosophila visual system is, hence, an invaluable starting point for comparative studies across insects.

Figure 1

OUR TOOLS

We are using the Drosophila genetic arsenal (Figure 3) and sc-Seq approaches (Figure 2) as tools to answer our questions. Technological developments in scSeq approaches and in transcriptome analysis give us for the first time the opportunity to understand neuronal diversity and get access to the transcriptomes of almost all cell types in the brain. Moreover, recent research has catalogued almost all neurons in the adult brain in Drosophila melanogaster. Apart from generating atlases, significant biological insights have arisen from scSeq studies. For example, we unraveled new causal relationships between transcription factors and terminal features and found that a specific neuronal feature (e.g. neurotransmitter identity) can be the endpoint of several distinct developmental trajectories (we termed this phenotypic convergence). Similar studies in other animals have illustrated the power of scSeq to address a variety of biological questions.

Figure 2

MORE DETAILS ABOUT THE DROSOPHILA VISUAL SYSTEM

The fly optic lobes can be divided into four neuropils: lamina, medulla, lobula and lobula plate (Figure 1). The lamina receives direct innervation from photoreceptors before relaying information to the medulla and to higher processing centers for motion detection. The lamina neurons, as well as two photoreceptors, project to the medulla. With ~40,000 cells belonging to over 100 cell types, the medulla is the most complex structure in the optic lobe. 800 ‘columns’ represent the functional units of the medulla that are processing visual information coming from one point in space in a retinotopic manner. The retinotopic organization of the medulla is somehow preserved in the lobula complex, where motion (lobula plate) and other visual features discussed in detail later (lobula), are processed.

Figure 3

The vast majority of Drosophila optic lobe neurons are generated during the third larval stage. Two neuroepithelial structures generate neural stem cells (neuroblasts) that divide many times, self-renewing and generating an intermediate precursor, called ganglion mother cell (GMC), which divides only once to generate two neurons. Neuroblasts are capable of generating many different neuronal types using three main mechanisms. i) Temporal patterning: eleven transcription factors (TFs), Oaz, Hth, Opa, Erm, Ey, Hbn, Slp, Scro, D, BarHI, and Tll (Figure 4) are expressed in a tight temporal sequence in every neuroblast, leading to the formation of the different neuronal populations. ii) Spatial patterning: three TFs expressed in sub-regions of the neuroepithelium (Vsx, Optix, Rx) that generate the medulla modify the neuroblast identity without affecting the temporal sequence to generate locally more neuronal types. iii) Notch-driven binary cell fate decision: further neuronal diversity is achieved by Notch during the last asymmetric division of the GMC. The combination of these three mechanisms, as well as cell death, are responsible for the generation of a large proportion of the observed neuronal diversity.

Figure 4

We are interested in understanding how these neuronal specification mechanisms have evolved and how they diversify to generate novel cell types.

Group Leader:

Nikos KONSTANTINIDES

Email:  nikos.konstantinides(at)ijm.fr
Phone: +33 (0) 1 57 27 81 45

 

Members:

Konstantina FILIPPOPOULOU Researcher
Nicola GRILLENZONI Biological engineer
Elisavet ILIOPOULOU Biological engineer

Konstantinides N.*,$, Holguera I. $, Rossi A.M. ,$, Escobar A., Dudragne L., Chen Y.C., Tran T., Martinez Jaimes A., Özel M.N., Simon F., Shao Z., Tsankova N.M., Fullard J.F., Walldorf U., Roussos P., Desplan C.* (2021) A comprehensive series of temporal transcription factors in the fly visual system.

Nature (accepted)

$ co-first author, * co-corresponding author

Simon F.*, Konstantinides N.* (2021) Single-cell transcriptomics in the Drosophila visual system: advances and perspectives on cell identity regulation, connectivity, and neuronal diversity evolution.

Developmental Biology 479:107-122

* co-corresponding author

Özel M.N., Jafari S., Holguera I., Chen Y.C, Benhra N., El-Danaf R.N., Kapuralin K.,  Malin J.A., Konstantinides N.*, Desplan C. (2021) Transient and heterogeneous neuronal types revealed by a comprehensive developmental atlas of the Drosophila optic lobe.

Nature 589(7840):88-95

* co-corresponding author

Konstantinides N.*, and Desplan C.* (2020) Neuronal differentiation strategies: insights from single-cell sequencing and machine learning.

Development 147: dev193631

* co-corresponding author

Konstantinides N.*, Kapuralin K., Fadil C., Barboza L., Satija R., Desplan C. (2018) Phenotypic convergence in the brain: distinct transcription factors regulate common terminal neuronal characters.

Cell 174(3):622-635.

* co-corresponding author

Konstantinides N.*, Degabriel S., Desplan C. (2018) Neuro-evo-devo in the single cell sequencing era.

Curr Opin Syst Biol 11:32-40.

* corresponding author

Konstantinides N.* and Averof M. (2014) A common cellular basis for muscle regeneration in arthropods and vertebrates.

Science 343(6172):788-91

* co-corresponding

Since 2015:

Konstantinides N., Rossi A.M. and Desplan C. (2015) Common temporal identity factors regulate neuronal diversity in fly ventral nerve cord and mouse retina.

Neuron 85(3):447-9

Courgeon M., Konstantinides N. and Desplan C. (2015) Cell competition: dying for communal interest.

Current Biology 25(8):R339-41

Grillo M., Konstantinides N.*, Averof M. (2016) Old questions, new models: unraveling complex organ regeneration with new experimental approaches.

Curr Opin Genet Dev. 40:23-31.

* co-corresponding author

Pinto-Teixeira F.$Konstantinides N.*$, Desplan C. (2016) Programmed cell death acts at different stages of Drosophila neurodevelopment to shape the central nervous system.

FEBS Lett. 590(15):2435-53

equal contribution * co-corresponding author

Kao D., Lai A.G., Stamataki E., Rosic S., Konstantinides N., et al. (2016) The genome of the crustacean Parhyale hawaiensis: a model for animal development, regeneration, immunity and lignocellulose digestion.

Elife. 5. pii: e20062

Konstantinides N. A mathematical approach for secondary structure analysis can provide an eyehole to the RNA world.

Biorxiv doi: https://doi.org/10.1101/079608

Perry M., Konstantinides N., Pinto-Teixeira F., Desplan C. (2017) Generation and evolution of neural cell types and circuits: insights from the Drosophila visual system.

Annu Rev Genet. 51:501-527

Konstantinides N.*, Kapuralin K., Fadil C., Barboza L., Satija R., Desplan C. (2018) Phenotypic convergence in the brain: distinct transcription factors regulate common terminal neuronal characters.

Cell 174(3):622-635.

* co-corresponding author

Davie K., Janssens J., Koldere D., De Waegeneer M., Pech U., Kreft L., Aibar S., Makhzami S., Christiaens V., Bravo González-Blas C., Poovathingal S., Hulselmans G., Spanier K.I., Moerman T., Vanspauwen B., Geurs S., Voet T., Lammertyn J., Thienpont B., Liu S., Konstantinides N., Fiers M., Verstreken P., and Aerts S. (2018) A single-cell transcriptome atlas of the ageing Drosophila brain. Cell 174(4):982-998.

Konstantinides N.*, Degabriel S., Desplan C. (2018) Neuro-evo-devo in the single cell sequencing era.

Curr Opin Syst Biol 11:32-40.

* corresponding author

Özel M.N., Jafari S., Holguera I., Chen Y.C, Benhra N., El-Danaf R.N., Kapuralin K.,  Malin J.A., Konstantinides N.*, Desplan C. (2021) Transient and heterogeneous neuronal types revealed by a comprehensive developmental atlas of the Drosophila optic lobe.

Nature 589(7840):88-95

* co-corresponding author

Konstantinides N.*, and Desplan C.* (2020) Neuronal differentiation strategies: insights from single-cell sequencing and machine learning.

Development 147: dev193631

* co-corresponding author

Opachaloemphan C.*, Mancini M.*, Konstantinides N.*, Parikh A., Mlejnek J., Yan H., Reinberg D., Desplan C. (2021) Early behavioral and molecular events leading to caste switching in the ant Harpegnathos.

Genes and Development 35: 410-424.

* co-first author

Konstantinides N.*, Rossi A.M., Escobar A., Dudragne L., Chen Y.C., Tran T., Martinez Jaimes A., Özel M.N., Simon F., Shao Z., Tsankova N.M., Fullard J.F., Walldorf U., Roussos P., Desplan C.* (2021) A comprehensive series of temporal transcription factors in the fly visual system.

Biorxiv doi: https://doi.org/10.1101/2021.06.13.448242

* co-corresponding author

Simon F.*, Konstantinides N.* (2021) Single-cell transcriptomics in the Drosophila visual system: advances and perspectives on cell identity regulation, connectivity, and neuronal diversity evolution.

Developmental Biology 479:107-122

* co-corresponding author

Marques G.S., Teles-Reis J., Konstantinides N., Brito P.H., Homem C.C.F. (2021) Fate transitions in Drosophila neural lineages: a single cell road map to mature neurons.
Biorxiv doi: https://doi.org/10.1101/2021.06.22.449317

Li H., Janssens J., De Waegeneer M., Kolluru S.S., Davie K., Gardeux V., Saelens W., David F., Brbić M., Leskovec J., McLaughlin C.N., Xie Q., Jones R.C., Brueckner K., Shim J., Tattikota S.G., Schnorrer F., Rust K., Nystul T.G., Carvalho-Santos Z., Ribeiro C., Pal S., Przytycka T.M., Allen A.M., Goodwin S.F., Berry C.W., Fuller M.T., White-Cooper H., Matunis E.L., DiNardo S., Galenza A., O’Brien L.E., Dow J.A.T., FCA Consortium, Jasper H., Oliver B., Perrimon N., Deplancke B., Quake S.R., Luo L., Aerts S. (2022) Fly Cell Atlas: a single-nucleus transcriptomic atlas of the adult fruit fly.

Science 375(6584):eabk2432

Cheng Y.C.*, Konstantinides N.* (2022) Integration of spatial and temporal patterning in the invertebrate and vertebrate nervous system.
Frontiers in Neuroscience 16: https://doi.org/10.3389/fnins.2022.854422
* co-corresponding author

  • Toni Gabaldon (IRB, Barcelona)
  • Michael Perry (University of California San Diego)