Chromatin Dynamics in Mammalian Development



DNA methylation is a stable epigenetic mark that is absolutely essential for proper mammalian development. In the Greenberg lab, the primary focus is understanding the various mechanisms and functions of DNA methylation in the context of development. We take a multidisciplinary approach, utilizing genetics, genomics, and biochemistry. We also strive to comprehend how DNA methylation interacts with other chromatin pathways to gain a holistic picture of chromatin-based gene regulation


Group Leader: Maxim Greenberg

Research Director CNRS

ORCiD: 0000-0001-9935-8763

Office: +33 (0) 1 57 27 81 20
Lab: +33 (0) 1 57 27 80 32







Research Topics:


Biologie quantitative et modélisation (Bioinformatics, Transcriptomics), Développement et évolution (Stem cells, Cell fate and differentiation), Dynamique du génome et des chromosomes (Epigenetics, Gene expression)


Selected publications


Greenberg M.V.C. (2021) Get Out and Stay Out: New Insights into DNA Methylation Reprogramming in Mammals. Front. Cell Dev. Biol. 8:629068


Greenberg M.V.C, Bourc’his D. (2019) The diverse roles of DNA methylation in mammalian development and disease. Nature Reviews Molecular Cell Biology 20:590-607


Greenberg M.V.C.†, Teissandier A., Walter, M., Noordermeer D., Bourc’his D†. (2019) Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency. eLife 8:e44057


Greenberg M.V.C.*, Glaser J.*, Borsos M., El Marjou F., Walter M., Teissandier A., Bourc’his D. (2017) Transient transcription in the early embryo sets an epigenetic state that programs postnatal growth. Nature Genet. 49:110-118

F1000Prime recommendation –


Greenberg M.V.C., Bourc’his D. (2015) Cultural relativism: maintenance of genomic imprints in pluripotent stem cell culture systems. Curr. Op. Genet. & Dev. 31:42–49


Greenberg M.V.C.*, Deleris A.*, Hale C.J.*, Liu A., Feng S., Jacobsen S.E. (2013) Interplay between active chromatin marks and RNA-directed DNA methylation in Arabidopsis thalianaPLoS Genet. 9(11): e1003946, 1-11


Stroud H., Greenberg M.V.C., Feng S., Bernatavichute Y.V., Jacobsen S.E. (2013) Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome. Cell 152: 352-64


Ausin I.*, Greenberg M.V.C.*, Simanshu D.K.*, Hale C.J., Vashisht A.A., Simon S.A., Lee T., Feng S., Espanola S.D., Meyers B.C., Wohlschlegel J.A., Patel D.J., Jacobsen S.E. (2012) INVOLVED IN DE NOVO 2 containing complex involved in RNA-directed DNA methylation in ArabidopsisProc. Nat. Acad. Sci. U. S. A. 109: 8374-8381

Greenberg M.V.C., Ausin I., Chan S.W.L., Cokus S.J., Cuperus J.T., Feng S., Law J.A., Chu C., Pellegrini M., Carrington J.C., Jacobsen S.E. (2011) Identification of genes required for de novo DNA methylation in ArabidopsisEpigenetics6:344-354


Deleris A.*, Greenberg M.V.C.*, Ausin I., Law R.W.Y., Moissiard G., Schubert D., Jacobsen S.E. (2010) Involvement of a Jumonji-C domain-containing histone demethylase in DRM2-mediated maintenance of DNA Methylation. EMBO Rep.11: 950-955