Epigenome and Paleogenome

THIERRY GRANGE & EVA-MARIA GEIGL

« Nothing in biology makes sense except in the light of evolution” (Theodosius Dobzhansky, 1900-1975). Our overarching research theme is the study of the evolution of genomes in effort to deepen our understanding of the relationship between genotypes and phenotypes’ aiming at deepening our understanding of the evolutionary relationship between genotypes and phenotypes. Recent phenotypic adapatations are ideal to identify the underlying genomic evolution while minimizing the the confounding effects of genetic drift. Our laboratory is currently working on several research projects concerning the recent evolution of genomes through the analysis of ancient genomes from fossils, the direct witnesses of evolution. This paleogenomic approach adds a geological or historical time-scale to evolutionary studies anchoring and rendering more robust evolutionary modeling from present-day genomes. We use and develop paleogenetic and paleogenomic approaches to document evolutionary processes occurring over the last hundreds of thousands of years and to push methodological limits to enable the study of more ancient samples and fossils from geographic regions where the hot climate is particularly detrimental to DNA preservation.

We focus on two major research lines: 1) We study animal genomes throughout their domestication processes, as domestication represents a unique, accelerated and human-driven model of evolution of specific genomic regions. 2) We study the genomic evolution of human populations during the peopling of Europe since the last Ice Age.

Keywords : evolution, genomes, ancient DNA, paleogenomics, peopling, domestication

+33 (0)157278129      thierry.grange (at) ijm.fr / eva-maria.geigl (at) ijm.fr     @ThierryGrange5

« Nothing in biology makes sense except in the light of evolution” (Theodosius Dobzhansky, 1900-1975). Our overarching research theme is the study of the evolution of genomes in effort to deepen our understanding of the relationship between genotypes and phenotypes’ aiming at deepening our understanding of the evolutionary relationship between genotypes and phenotypes. Recent phenotypic adapatations are ideal to identify the underlying genomic evolution while minimizing the the confounding effects of genetic drift. Our laboratory is currently working on several research projects concerning the recent evolution of genomes through the analysis of ancient genomes from fossils, the direct witnesses of evolution. This paleogenomic approach adds a geological or historical time-scale to evolutionary studies anchoring and rendering more robust evolutionary modeling from present-day genomes. We use and develop paleogenetic and paleogenomic approaches to document evolutionary processes occurring over the last hundreds of thousands of years and to push methodological limits to enable the study of more ancient samples and fossils from geographic regions where the hot climate is particularly detrimental to DNA preservation.

We focus on two major research lines: 1) We study animal genomes throughout their domestication processes, as domestication represents a unique, accelerated and human-driven model of evolution of specific genomic regions. 2) We study the genomic evolution of human populations during the peopling of Europe since the last Ice Age.

We have developed five interdisciplinary research themes:

  1. Paleogenomic study of the dynamics of bovine populations during the Pleistocene influenced by climate and humans. Through the analysis of mitochondrial and nuclear genomes in up to 150,000-year-old fossils, we retrace migrations, contractions, expansions, extinctions and replacements of aurochs and bison populations. We aim to discriminate between environmental and human-induced effects on the evolution of these large herbivore populations by comparing the two sister-species, Bos and Bison, as only Bos (the aurochs) was domesticated.
  2. Evolution of the bovine genome during domestication. We use paleogenomic approaches to reconstruct the genomic modifications that have occurred over the last ~9 000 years during the domestication of the aurochs, the wild ancestor of present-day cattle. We are characterizing in time and space the selection exerted by different ancient societies on particular genomic regions thus shaping the genomic make-up of present-day cattle breeds.
  3. Evolution of the cat genome during domestication. Using ancient genomes, we attempt to reconstruct the domestication history of the cat over the last ~10,000 years, which is very different from the one of cattle. The cat’s domestication largely followed the commensal pathway and selective breeding became a prominent domestication mechanism only very recently. Currently, we are also characterizing the genomic adaptation of the domestic cat alongside its interaction and hybridization with the European wildcat.
  4. Paleogenomics of the Neolithic of the Paris Basin. We analyze the genomes of individuals who lived in the Paris Basin between ~7,000 and 4,500 years ago to reconstruct the evolution of their corresponding populations, along with the gene flow, admixture and migrations that took place during this period. This study lends us broader insight into the evolution of the peopling of France as the Neolithic represents a key period for the development of societies leading to the present.
  5. Paleogenomics of the evolution of disease susceptibility and resistance on the territory of present-day France since the Neolithic. We are analyzing the co-evolution of humans and cattle and the influence of epidemics, including bovine zoonoses, on the genomes of the populations in the north and west of France from the Neolithic, 7,200 years ago, to the present.

Group Leaders:

Thierry GRANGE
Téléphone : +33 (0)157278129
Email : thierry.grange (at) ijm.fr

Eva-Maria GEIGL
Téléphone : +33 (0)157278132
Email : eva-maria.geigl (at) ijm.fr

 

Members:

Fabien

Lesley Caitlin

FAUCHEREAU

MARTIN

Researcher

PhD Student

Jeanne MATTEI PhD Student
Oguzhan PARASAYAN PhD Student
Sabine ROUSSELOT Biological engineer

Bennett, E.A., Weber, J., Bendhafer, W., Champlot, S., Peters, J., Schwartz, G., Grange, T., Geigl, E.-M. (2022) The genetic identity of the earliest human-made hybrid animals, the kungas of Syro-Mesopotamia. Science Advances 8, eabm0218. DOI : 10.1126/sciadv.abm0218

Guimaraes, S., Arbuckle, B., Peters, J., Adcock, S., Buitenhuis, H., Chavin, H., Manaseryan, N., Uerpmann, H.-P., Grange, T.*, Geigl, E.-M.* (2020) Ancient DNA shows domestic horses were introduced in the southern Caucasus and Anatolia during the Bronze Age. Science Advances, 6: eabb0030, DOI: 10.1126/sciadv.abb0030

Brunel, S., Bennett, E.A., Cardin, L., Garraud, D., Barrand Emam, H., Beylier, A., Boulestin, B., Chenal, F., Cieselski, E., Convertini, F., Dedet, B., Desenne, S., Dubouloz, J., Duday, H., Fabre, V., Gailledrat, E., Gandelin, M., Gleize, Y., Goepfert, S., Guilaine, J., Hachem, L., Ilett, M., Lambach, F., Mazière, F., Perrin, B., Plouin, S., Pinard, E., Praud, I., Richard, I., Riquier, V., Roure, R., Sendra, B., Thevenet, C., Thiol, S., Vauquelin, E., Vergnaud, L., Grange, T.*, Geigl, E.-M.*, Pruvost, M.* (2020) Ancient genomes from present-day France unveil 7,000 years of its demographic history. Proceedings of the National Academy of Sciences USA, 117(23):12791-12798, DOI:10.1073/pnas.1918034117.

Bennett, E.A., Crevecoeur, I., Viola, B., Derevianko, A.P., Shunkov, M.V., Grange, T., Maureille, B., Geigl, E.-M. (2019) Morphology of the Denisovan phalanx closer to modern humans than to Neandertals. Science Advances, 5:eaaw3950, DOI 10.1126/sciadv.aaw3950, DOI: 10.1126/sciadv.aaw3950

Grange, T., Brugal, J.-P., Flori, L., Gautier, M., Uzunidis, A., Geigl, E.-M. (2018) The Evolution and Population Diversity of Bison in Pleistocene and Holocene Eurasia: Sex Matters. Diversity 10, 65; doi:10.3390/d10030065

Ottoni, C., Van Neer, W., De Cupere, B., Daligault, J., Guimaraes, S., Peters, J., Spassov, N., Prendergast, M.E., Boivin, N., Morales-Muniz, A., Bălăşescu, A., Becker, C., Benecke, N., Boronenanț, A., Buitenhuis, H., Chahoud, J., Crowther, A., Llorente, L., Manaseryan, N., Monchot, H., Onar, V., Osypińska, M., Putelat, O., Studer, J., Wierer, U., Decorte, R., Grange, T.*, Geigl, E.-M.* (2017) The paleogenetics of cat dispersal in the ancient world. Nature Ecology & Evolution 1, 0139. DOI: https://doi.org/10.1038/s41559-017-0139.

Bennett, E.A., Champlot, S., Peters, J., Arbuckle, B.S., Guimaraes, S., Pruvost, M., Bar-David, S., Davis, S.J.M., Gautier, M., Kaczensky, P., Kuehn, R., Mashkour, M., Morales-Muñiz, M., Pucher, E., Tournepiche, J.-F., Uerpmann, H.-P., Bălăşescu, A., Germonpré, M., Y. Günden, C., Hemami, M.-R., Moullé, P.-E., Öztan, A., Uerpmann, M., Walzer, C., Grange, T.*, Geigl, E.-M.* (2017) Taming the Late Quaternary phylogeography of the Eurasiatic wild ass through ancient and modern DNA. Plos one 12(4): e0174216. /doi.org/10.1371/journal.pone.0174216

Massilani, D., Guimaraes, S., Brugal, J.-P., Bennett, E.A., Tokarska, M., Arbogast, R., Baryshnikov, G., Boeskorov, G., Castel, J.-C., Davydov, S., Madelaine, S., Putelat, O., Spasskaya, N., Uerpmann, H.-P., Grange, T.*, Geigl,E.-M.* (2016) Past climate changes, population dynamics and the origin of Bison in Europe. BMC Biology 14:93-110. DOI 10.1186/s12915-016-0317-7

Guimaraes S, Pruvost M, Daligault J, Stoetzel E, Bennett EA, Côté NM, Nicolas V, Lalis A, Denys C, Geigl EM*, Grange T.* (2017) A cost-effective high-throughput metabarcoding approach powerful enough to genotype ~44 000 year-old rodent remains from Northern Africa. Mol Ecol Resour. 17: 405-417. doi: 10.1111/1755-0998.12565

Champlot S., Berthelot C., Pruvost M., Bennett E. A., Grange T. and Geigl E.M. (2010) An Efficient Multistrategy DNA Decontamination Procedure of PCR reagents for Hypersensitive PCR Applications. PLOS One, 5(9), e13042.

Pruvost, M., Schwarz, R., Bessa Correia, V., Champlot, S., Braguier, S., Morel, N., Fernandez-Jalvo, Y., Grange, T., Geigl, E.-M. (2007) Freshly excavated fossil bones are best for amplification of ancient DNA. Proc. Ntl. Acad. Sci. USA, 104, 739-744.

*shared senior authors

Publications significatives de l’équipe :

Bennett, E.A., Weber, J., Bendhafer, W., Champlot, S., Peters, J., Schwartz, G., Grange, T., Geigl, E.-M. (2022) The genetic identity of the earliest human-made hybrid animals, the kungas of Syro-Mesopotamia. Science Advances 8, eabm0218. DOI : 10.1126/sciadv.abm0218

 

Guimaraes, S., Arbuckle, B., Peters, J., Adcock, S., Buitenhuis, H., Chavin, H., Manaseryan, N., Uerpmann, H.-P., Grange, T.*, Geigl, E.-M.* (2020) Ancient DNA shows domestic horses were introduced in the southern Caucasus and Anatolia during the Bronze Age. Science Advances, 6: eabb0030, DOI: 10.1126/sciadv.abb0030

 

Brunel, S., Bennett, E.A., Cardin, L., Garraud, D., Barrand Emam, H., Beylier, A., Boulestin, B., Chenal, F., Cieselski, E., Convertini, F., Dedet, B., Desenne, S., Dubouloz, J., Duday, H., Fabre, V., Gailledrat, E., Gandelin, M., Gleize, Y., Goepfert, S., Guilaine, J., Hachem, L., Ilett, M., Lambach, F., Mazière, F., Perrin, B., Plouin, S., Pinard, E., Praud, I., Richard, I., Riquier, V., Roure, R., Sendra, B., Thevenet, C., Thiol, S., Vauquelin, E., Vergnaud, L., Grange, T.*, Geigl, E.-M.*, Pruvost, M.* (2020) Ancient genomes from present-day France unveil 7,000 years of its demographic history. Proceedings of the National Academy of Sciences USA, 117(23):12791-12798, DOI:10.1073/pnas.1918034117.

 

Bennett, E.A., Crevecoeur, I., Viola, B., Derevianko, A.P., Shunkov, M.V., Grange, T., Maureille, B., Geigl, E.-M. (2019) Morphology of the Denisovan phalanx closer to modern humans than to Neandertals. Science Advances, 5:eaaw3950, DOI 10.1126/sciadv.aaw3950, DOI: 10.1126/sciadv.aaw3950

 

Grange, T., Brugal, J.-P., Flori, L., Gautier, M., Uzunidis, A., and Geigl, E.-M. (2018) The Evolution and Population Diversity of Bison in Pleistocene and Holocene Eurasia: Sex Matters. Diversity 10, 65; doi:10.3390/d10030065

 

Ottoni, C., Van Neer, W., De Cupere, B., Daligault, J., Guimaraes, S., Peters, J., Spassov, N., Prendergast, M.E., Boivin, N., Morales-Muniz, A., Bălăşescu, A., Becker, C., Benecke, N., Boronenanț, A., Buitenhuis, H., Chahoud, J., Crowther, A., Llorente, L., Manaseryan, N., Monchot, H., Onar, V., Osypińska, M., Putelat, O., Studer, J., Wierer, U., Decorte, R., Grange, T.*, Geigl, E.-M.* (2017) The paleogenetics of cat dispersal in the ancient world. Nature Ecology & Evolution 1, 0139. DOI: https://doi.org/10.1038/s41559-017-0139.

 

Bennett, E.A., Champlot, S., Peters, J., Arbuckle, B.S., Guimaraes, S., Pruvost, M., Bar-David, S., Davis, S.J.M., Gautier, M., Kaczensky, P., Kuehn, R., Mashkour, M., Morales-Muñiz, M., Pucher, E., Tournepiche, J.-F., Uerpmann, H.-P., Bălăşescu, A., Germonpré, M., Y. Günden, C., Hemami, M.-R., Moullé, P.-E., Öztan, A., Uerpmann, M., Walzer, C., Grange, T.*, Geigl, E.-M.* (2017) Taming the Late Quaternary phylogeography of the Eurasiatic wild ass through ancient and modern DNA. Plos one 12(4): e0174216. /doi.org/10.1371/journal.pone.0174216

 

Guimaraes S, Pruvost M, Daligault J, Stoetzel E, Bennett EA, Côté NM, Nicolas V, Lalis A, Denys C, Geigl E.-M.*, Grange T.* (2017) A cost-effective high-throughput metabarcoding approach powerful enough to genotype ~44 000 year-old rodent remains from Northern Africa. Mol Ecol Resour. 17: 405-417. doi: 10.1111/1755-0998.12565

 

Massilani, D., Guimaraes, S., Brugal, J.-P., Bennett, E.A., Tokarska, M., Arbogast, R., Baryshnikov, G., Boeskorov, G., Castel, J.-C., Davydov, S., Madelaine, S., Putelat, O., Spasskaya, N., Uerpmann, H.-P., Grange, T.*, Geigl,E.-M.* (2016) Past climate changes, population dynamics and the origin of Bison in Europe. BMC Biology 14:93-110. DOI 10.1186/s12915-016-0317-7

 

Côté, N.M.L., Daligault, J., Pruvost, M., Bennett, E.A., Gorgé, O., Guimaraes, S., Capelli, N., Le Bailly, M., Geigl, E.-M.*, Grange, T.* (2016) A New High-Througput Approach to Genotype Ancient Human Gastrointestinal Parasites.  PLoS One. 2016 11(1):e0146230. doi: 10.1371/journal.pone.0146230. eCollection 2016.

 

Bennett, E.A., Massilani, D., Lizzo, G., Daligault, J., Geigl, E.-M., Grange, T. (2014) Library construction for ancient genomics: single strand or double strand? Biotechniques 56:289-300. DOI: 10.2144/000114176

Grange T. and Lourenço E. E. (2011) Mechanisms of Epigenetic Gene Activation in Disease: Dynamics of DNA Methylation and Demethylation, in Epigenetic Aspects of Chronic Diseases; pp 55-73; Roach H.I., Bronner F. and Oreffo R. O. eds, Springer, London.

Charruau P, Fernandes C, Orozco-Terwengel P, Peters J, Hunter L, Ziaie H, Jourabchian A, Jowkar H, Schaller G, Ostrowski S, Vercammen P, Grange T, Schlötterer C, Kotze A, Geigl, E.-M., Walzer C, Burger PA. (2010) Phylogeography, genetic structure and population divergence time of cheetahs in Africa and Asia: evidence for long-term geographic isolates. Mol Ecol. (4):706-724

Champlot S., Berthelot C., Pruvost M., Bennett E. A., Grange T. and Geigl E.M. (2010). An Efficient Multistrategy DNA Decontamination Procedure of PCR reagents for Hypersensitive PCR Applications. PLOS One, 5(9): e13042.

Mietton F, Sengupta AK, Molla A, Picchi G, Barral S, Heliot L, Grange T, Wutz A, Dimitrov S. (2009) Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. Mol Cell Biol. 29, 150-156

Grange T. (2008) Sensitive Detection of mRNA Decay Products Using Reverse-Ligation-Mediated PCR (RL-PCR). in Methods in Enzymology, RNA Turnover in Eukaryotes, Part B, 448, 445-466

Pruvost, M., Schwarz, R., Bessa Correia, V., Champlot, S., Grange, T. and Geigl, E.-M. (2008). DNA diagenesis and palaeogenetic analysis: critical assessment and methodological progress. Palaeogeography, Palaeoclimatology, Palaeoecology, 266, 211-219.

Miele V., C. Vaillant, Y. d’Aubenton-Carafa, C. Thermes and T. Grange (2008). DNA physical properties determine nucleosome occupancy from yeast to fly. Nucl Acids Res, 36, 3746-3756

Pruvost, M., T. Grange and E. M. Geigl (2005). “Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: application to ancient DNA studies.” Biotechniques 38(4): 569-75.
abstract

Thomassin, H., C. Kress and T. Grange (2004). “MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome.” Nucleic Acids Res 32(21).
abstract

Flavin, M., L. Cappabianca, C. Kress, H. Thomassin and T. Grange (2004). “Nature of the accessible chromatin at a glucocorticoid-responsive enhancer.” Mol Cell Biol 24(18): 7891-901.
abstract

Pruvost, M. and E. M. Geigl (2004). “Real-time quantitative PCR to assess the authenticity of ancient DNA amplification.” J. Archaeol. Sci. 31(9): 1191-1197.
abstract

Couttet, P. and T. Grange (2004). “Premature termination codons enhance mRNA decapping in human cells.” Nucleic Acids Res 32(2): 488-94. Print 2004.
abstract

Grange, T., L. Cappabianca, M. Flavin, H. Sassi and H. Thomassin (2001). “In vivo analysis of the model tyrosine aminotransferase gene reveals multiple sequential steps in glucocorticoid receptor action.” Oncogene 20(24): 3028-38.
abstract

Geigl, E.-M. (2002) “On the Circumstances Surrounding the Preservation and Analysis of Very Old DNA”. Archaeometry 44 (3) :337-342,

Thomassin, H., M. Flavin, M. L. Espinas and T. Grange (2001). “Glucocorticoid-induced DNA demethylation and gene memory during development.” Embo J 20(8): 1974-83.
abstract

Geigl, E. M. (2001). “Inadequate use of molecular hybridization to analyze DNA in Neanderthal fossils.” Am J Hum Genet 68(1): 287-91.
abstract

Christoffels, V. M., H. Sassi, J. M. Ruijter, A. F. Moorman, T. Grange and W. H. Lamers (1999). “A mechanistic model for the development and maintenance of portocentral gradients in gene expression in the liver.” Hepatology 29(4): 1180-92.
abstract

Sassi, H., R. Pictet and T. Grange (1998). “Glucocorticoids are insufficient for neonatal gene induction in the liver.” Proc Natl Acad Sci U S A 95(10): 5621-5.
abstract

Couttet, P., M. Fromont-Racine, D. Steel, R. Pictet and T. Grange (1997). “Messenger RNA deadenylylation precedes decapping in mammalian cells.” Proc Natl Acad Sci U S A 94(11): 5628-33.
abstract

De Sario, A., Geigl, E.-M., Palmieri, G., D’Urso, M., Bernardi, G. (J’ai co-encadré l’étudiante en these A. DeSario)A compositional map of human chromosome band Xq28. Proc. Natl. Acad. Sci. USA 93:1298-1302, 1996

 

Francke, U., Chang, E., Comeau, K., Geigl, E.-M., Giacalone, J., Li, X., Luna, J., Moon, A., Welch, S., Wilgenbus, P. (auteurs listés par ordre alphabétique) (1994) A radiation hybrid map of human chromosome 18. Cytogenet Cell Genet 66:196-213.

 

Leib-Mösch, C., Haltmeier, M., Werner, T., Geigl, E.-M., Brack-Werner, R., Francke, U., Erfle, V., Hehlmann, R. (1993) Genomic distribution and transcription of solitary HERV-K LTRs. Genomics 18:261-269.

 

Geigl, E.-M., Eckardt-Schupp, F. (1990) Chromosome-specific identification and quantification of S1 nuclease-sensitive sites in yeast chromatin by pulsed field gel electrophoresis. Molecular Microbiology 4(5):801-810.

 

PUBLICATIONS THIERRY GRANGE

 

Guimaraes, S., Arbuckle, B., Peters, J., Adcock, S., Buitenhuis, H., Chavin, H., Manaseryan, N., Uerpmann, H.-P., Grange, T.*, Geigl, E.-M.* (2020) Ancient DNA shows domestic horses were introduced in the southern Caucasus and Anatolia during the Bronze Age. Science Advances, 6: eabb0030, DOI: 10.1126/sciadv.abb0030

 

Brunel, S., Bennett, E.A., Cardin, L., Garraud, D., Barrand Emam, H., Beylier, A., Boulestin, B., Chenal, F., Cieselski, E., Convertini, F., Dedet, B., Desenne, S., Dubouloz, J., Duday, H., Fabre, V., Gailledrat, E., Gandelin, M., Gleize, Y., Goepfert, S., Guilaine, J., Hachem, L., Ilett, M., Lambach, F., Mazière, F., Perrin, B., Plouin, S., Pinard, E., Praud, I., Richard, I., Riquier, V., Roure, R., Sendra, B., Thevenet, C., Thiol, S., Vauquelin, E., Vergnaud, L., Grange, T.*, Geigl, E.-M.*, Pruvost, M.* (2020) Ancient genomes from present-day France unveil 7,000 years of its demographic history. Proceedings of the National Academy of Sciences USA, 117(23):12791-12798, DOI:10.1073/pnas.1918034117.

 

Bennett, E.A., Crevecoeur, I., Viola, B., Derevianko, A.P., Shunkov, M.V., Grange, T., Maureille, B., Geigl, E.-M. (2019) Morphology of the Denisovan phalanx closer to modern humans than to Neandertals. Science Advances, 5:eaaw3950, DOI 10.1126/sciadv.aaw3950, DOI: 10.1126/sciadv.aaw3950

 

Geigl, E.-M., Grange, T. (2018) Ancient DNA: The quest for the best. Mol Ecol Resour. 18:1185–1187. DOI: 10.1111/1755-0998.12931

 

Grange, T., Brugal, J.-P., Flori, L., Gautier, M., Uzunidis, A., and Geigl, E.-M. (2018) The Evolution and Population Diversity of Bison in Pleistocene and Holocene Eurasia: Sex Matters. Diversity 10, 65; doi:10.3390/d10030065

 

Ottoni, C., Van Neer, W., De Cupere, B., Daligault, J., Guimaraes, S., Peters, J., Spassov, N., Prendergast, M.E., Boivin, N., Morales-Muniz, A., Bălăşescu, A., Becker, C., Benecke, N., Boronenanț, A., Buitenhuis, H., Chahoud, J., Crowther, A., Llorente, L., Manaseryan, N., Monchot, H., Onar, V., Osypińska, M., Putelat, O., Studer, J., Wierer, U., Decorte, R., Grange, T.*, Geigl, E.-M.* (2017) The paleogenetics of cat dispersal in the ancient world. Nature Ecology & Evolution 1, 0139. DOI: https://doi.org/10.1038/s41559-017-0139.

 

Bennett, E.A., Champlot, S., Peters, J., Arbuckle, B.S., Guimaraes, S., Pruvost, M., Bar-David, S., Davis, S.J.M., Gautier, M., Kaczensky, P., Kuehn, R., Mashkour, M., Morales-Muñiz, M., Pucher, E., Tournepiche, J.-F., Uerpmann, H.-P., Bălăşescu, A., Germonpré, M., Y. Günden, C., Hemami, M.-R., Moullé, P.-E., Öztan, A., Uerpmann, M., Walzer, C., Grange, T.*, Geigl, E.-M.* (2017) Taming the Late Quaternary phylogeography of the Eurasiatic wild ass through ancient and modern DNA. Plos one 12(4): e0174216. /doi.org/10.1371/journal.pone.0174216

 

Guimaraes S, Pruvost M, Daligault J, Stoetzel E, Bennett EA, Côté NM, Nicolas V, Lalis A, Denys C, Geigl EM*, Grange T.* (2017) A cost-effective high-throughput metabarcoding approach powerful enough to genotype ~44 000 year-old rodent remains from Northern Africa. Mol Ecol Resour. 17: 405-417. doi: 10.1111/1755-0998.12565

 

Massilani, D., Guimaraes, S., Brugal, J.-P., Bennett, E.A., Tokarska, M., Arbogast, R., Baryshnikov, G., Boeskorov, G., Castel, J.-C., Davydov, S., Madelaine, S., Putelat, O., Spasskaya, N., Uerpmann, H.-P., Grange, T.*, Geigl,E.-M.* (2016) Past climate changes, population dynamics and the origin of Bison in Europe. BMC Biology 14:93-110. DOI 10.1186/s12915-016-0317-7

 

Gorgé O, Bennett EA, Massilani D, Daligault J, Pruvost M, Geigl E.-M., Grange T. (2016) Analysis of Ancient DNA in Microbial Ecology. Methods Mol Biol. 1399:289-315. doi: 10.1007/978-1-4939-3369-3_17.

 

Côté, N.M.L., Daligault, J., Pruvost, M., Bennett, E.A., Gorgé, O., Guimaraes, S., Capelli, N., Le Bailly, M., Geigl*, E.-M., Grange, T.* (2016) A New High-Througput Approach to Genotype Ancient Human Gastrointestinal Parasites.  PLoS One. 2016 11(1):e0146230. doi: 10.1371/journal.pone.0146230. eCollection 2016.

 

Guimaraes, S., Fernandez-Jalvo, Y., Stoetzel, E., Gorgé, O., Bennett, E.A., Denys, C., Grange, T., Geigl, E.-M. (2016) Owl pellets: a wise DNA source for small mammal genetics. Journal of Zoology 298(1):64–74. doi:10.1111/jzo.12285

 

Bennett, E.A., Massilani, D., Lizzo, G., Daligault, J., Geigl, E.-M., Grange, T. (2014) Library construction for ancient genomics: single strand or double strand? Biotechniques 56:289-300. DOI: 10.2144/000114176

Geigl E. M. and Grange T. (2012) Eurasian wild asses in time and space: Morphological versus genetic diversity. Ann Anat. 194:88-102

Geigl, E.M., Grange, T., Maureille, B. : Le génome néandertalien. Encyclopaedia Universalis, « La Science au présent 2011 », p. 133-140

Charruau P, Fernandes C, Orozco-Terwengel P, Peters J, Hunter L, Ziaie H, Jourabchian A, Jowkar H, Schaller G, Ostrowski S, Vercammen P, Grange T, Schlötterer C, Kotze A, Geigl EM, Walzer C, Burger PA. (2011) Phylogeography, genetic structure and population divergence time of cheetahs in Africa and Asia: evidence for long-term geographic isolates. Mol Ecol. (4):706-724

Grange T. and Lourenço E. (2011) Mechanisms of Epigenetic Gene Activation in Disease: Dynamics of DNA Methylation and Demethylation, in Epigenetic Aspects of Chronic Diseases; pp 55-73; Roach H.I., Bronner F. and Oreffo R. O. eds, Springer, London.

Mietton F, Sengupta AK, Molla A, Picchi G, Barral S, Heliot L, Grange T, Wutz A, Dimitrov S. (2009) Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. Mol Cell Biol. 29, 150-156

Champlot S., Berthelot C., Pruvost M., Bennett E. A., Grange T. and Geigl E.M. (2010). An Efficient Multistrategy DNA Decontamination Procedure of PCR reagents for Hypersensitive PCR Applications. PLOS One, 5(9): e13042.

Grange T. (2008) Sensitive Detection of mRNA Decay Products Using Reverse-Ligation-Mediated PCR (RL-PCR). In Methods in Enzymology, RNA Turnover in Eukaryotes, Part B, 448, 445-466

Pruvost, M., Schwarz, R., Bessa Correia, V., Champlot, S., Grange, T. and Geigl, E.-M.(2008) DNA diagenesis and palaeogenetic analysis: critical assessment and methodological progress. Palaeogeography, Palaeoclimatology, Palaeoecology, 266, 211-219.

Miele V., C. Vaillant, Y. d’Aubenton-Carafa, C. Thermes and T. Grange (2008) DNA physical properties determine nucleosome occupancy from yeast to fly. Nucl. Acids Res, 36, 3746-3756

Dugast-Darzacq C., and T. Grange (2008) MethylQuant : a real time PCR based method to quantify methylation at single specific cytosines. In Methods in Molecular Biology, DNA methylation: Methods and protocols, Second edition, J. Tost Ed, Humana Press, 507, 281-303.

Dugast-Darzacq C., T. Grange and N. B. Schreiber-Agus (2007) Differential effects of Mxi1-SR and Mxi1-SR in Myc antagonism. FEBS J. 274, 4643-53.

Pruvost, R. Schwarz, V. Bessa Correia, S. Champlot, S. Braguier, N. Morel, Y. Fernandez-Jalvo, T. Grange and EM Geigl (2007) Freshly excavated fossil bones are best for amplification of ancient DNA M. Proc. Ntl. Acad. Sci. USA, 104, 739-744

Hoogenkamp M, Gaemers IC, Schoneveld OJ, Das AT, Grange T, Lamers WH (2007) Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction. FEBS J. 274, 37–45

Schalchi L., Grange T., Hänni, C., and Morange M. (2006) Bac to basics: l’ADN. La Recherche, 398, 75-78

Active cytosine demethylation triggered by a transcriptional regulator involves DNA strand breaks. Kress C., Thomassin H., and Grange T., (2006) Proc. Ntl. Acad. Sci. USA 103, 1112-1117.

Grange, T., J. Imbert and D. Thieffry (2005). “Epigenomics: large scale analysis of chromatin modifications and transcription factors/genome interactions.” Bioessays 27(11): 1203-5.
abstract

Flavin, M., L. Cappabianca, C. Kress, H. Thomassin and T. Grange (2004). “Nature of the accessible chromatin at a glucocorticoid-responsive enhancer.” Mol Cell Biol 24(18): 7891-901.
abstract

Thakur, N., V. K. Tiwari, H. Thomassin, R. R. Pandey, M. Kanduri, A. Gondor, T. Grange, R. Ohlsson and C. Kanduri (2004). “An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region.” Mol Cell Biol 24(18): 7855-62.
abstract

Thomassin, H., C. Kress and T. Grange (2004). “MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome.” Nucleic Acids Res 32(21).
abstract

Pâques F. and T. Grange (2002). Architecture nucléaire et régulation transcriptionnelle chez les eucaryotes supérieurs. Med./Sci 18, 1245-1256.
abstract

Grange, T. (2001). Traduction nucléaire : un garde-fou contre les protéines tronquées ?
Med./Sci. 17, 914-915.
abstract

Grange, T., L. Cappabianca, M. Flavin, H. Sassi and H. Thomassin (2001). “In vivo analysis of the model tyrosine aminotransferase gene reveals multiple sequential steps in glucocorticoid receptor action.” Oncogene 20(24): 3028-38.
abstract

Kress, C., H. Thomassin and T. Grange (2001). “Local DNA demethylation in vertebrates: how could it be performed and targeted’” FEBS Lett 494(3): 135-40.
abstract

Thomassin, H., M. Flavin, M. L. Espinas and T. Grange (2001). “Glucocorticoid-induced DNA demethylation and gene memory during development.” Embo J 20(8): 1974-83.
abstract

Cappabianca, L., H. Thomassin, R. Pictet and T. Grange (1999). “Genomic footprinting using nucleases.” Methods Mol Biol 119: 427-42.
abstract

Christoffels, V. M., H. Sassi, J. M. Ruijter, A. F. Moorman, T. Grange and W. H. Lamers (1999). “A mechanistic model for the development and maintenance of portocentral gradients in gene expression in the liver.” Hepatology 29(4): 1180-92.
abstract

Grange T.  and H. Thomassin (1999). « Comment déméthyler une cytosine? Enlève-t-on un peu, beaucoup, encore plus, ou pas du tout’ » Med./Sci., 15, 907-908.
abstract

Thomassin, H., E. J. Oakeley and T. Grange (1999). “Identification of 5-methylcytosine in complex genomes.” Methods 19(3): 465-75.
abstract

Christoffels, V. M., T. Grange, K. H. Kaestner, T. J. Cole, G. J. Darlington, C. M. Croniger and W. H. Lamers (1998). “Glucocorticoid receptor, C/EBP, HNF3, and protein kinase A coordinately activate the glucocorticoid response unit of the carbamoylphosphate synthetase I gene.” Mol Cell Biol 18(11): 6305-15.
abstract

Sassi, H., R. Pictet and T. Grange (1998). “Glucocorticoids are insufficient for neonatal gene induction in the liver.” Proc Natl Acad Sci U S A 95(10): 5621-5.
abstract

Bertrand, E., D. Castanotto, C. Zhou, C. Carbonnelle, N. S. Lee, P. Good, S. Chatterjee, T. Grange, R. Pictet, D. Kohn, D. Engelke and J. J. Rossi (1997). “The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization.” Rna 3(1): 75-88.
abstract

Bertrand, E., M. Fromont-Racine, R. Pictet and T. Grange (1997). “Detection of ribozyme cleavage products using reverse ligation-mediated PCR (RL-PCR).” Methods Mol Biol 74: 311-23.
abstract

Couttet, P., M. Fromont-Racine, D. Steel, R. Pictet and T. Grange (1997). “Messenger RNA deadenylylation precedes decapping in mammalian cells.” Proc Natl Acad Sci U S A 94(11): 5628-33.
abstract

Grange T., G. Rigaud, E. Bertrand, M. Fromont-Racine, M.-L. Espinás, J. Roux and R. Pictet (1997). “In vivo footprinting of the interaction of proteins with DNA and RNA.” in In vivo footprinting. Cartwright, I. L. Ed. Advances in Molecular and Cell Biology.  JAI Press, Inc., Greenwich, Conn. 21, 73-109.

Grange, T., E. Bertrand, M. L. Espinas, M. Fromont-Racine, G. Rigaud, J. Roux and R. Pictet (1997). “In vivo footprinting of the interaction of proteins with DNA and RNA.” Methods 11(2): 151-63.
abstract

Espinas, M. L., J. Roux, R. Pictet and T. Grange (1995). “Glucocorticoids and protein kinase A coordinately modulate transcription factor recruitment at a glucocorticoid-responsive unit.” Mol Cell Biol 15(10): 5346-54.
abstract

Roux, J., R. Pictet and T. Grange (1995). “Hepatocyte nuclear factor 3 determines the amplitude of the glucocorticoid response of the rat tyrosine aminotransferase gene.” DNA Cell Biol 14(5): 385-96.
abstract

Sassi, H., M. Fromont-Racine, T. Grange and R. Pictet (1995). “Tissue specificity of a glucocorticoid-dependent enhancer in transgenic mice.” Proc Natl Acad Sci U S A 92(16): 7197-201.
abstract

Bertrand, E., M. Fromont-Racine, R. Pictet and T. Grange (1993). “Visualization of the interaction of a regulatory protein with RNA in vivo.” Proc Natl Acad Sci U S A 90(8): 3496-500.
abstract

Bertrand, E., R. Pictet and T. Grange (1994). “Can hammerhead ribozymes be efficient tools to inactivate gene function?” Nucleic Acids Res 22(3): 293-300.
abstract

Espinas, M. L., J. Roux, J. Ghysdael, R. Pictet and T. Grange (1994). “Participation of Ets transcription factors in the glucocorticoid response of the rat tyrosine aminotransferase gene.” Mol Cell Biol 14(6): 4116-25.
abstract

Schweizer-Groyer, G., A. Groyer, F. Cadepond, T. Grange, E. E. Baulieu and R. Pictet (1994). “Expression from the tyrosine aminotransferase promoter (nt -350 to 1) is liver-specific and dependent on the binding of both liver-enriched and ubiquitous trans-acting factors.” Nucleic Acids Res 22(9): 1583-92.
abstract

Fromont-Racine, M., E. Bertrand, R. Pictet and T. Grange (1993). “A highly sensitive method for mapping the 5′ termini of mRNAs.” Nucleic Acids Res 21(7): 1683-4.
abstract

Bertrand E., T. Grange and R. Pictet (1992). “Trans-acting hammerhead ribozymes in vivo: present limits and future directions.” in Gene regulation by antisense RNA and DNA.  pp 71-81. R.P. Erickson & J. Izart Eds. Keistone Symposia series on Molecular and Cellular Biology. Raven Press, Ltd., New York.

Schweizer-Groyer, G., A. Groyer, F. Cadepond, T. Grange, E. E. Baulieu and R. Pictet (1992). “Two liver-enriched trans-acting factors support the tissue-specific basal transcription from the rat tyrosine aminotransferase promoter.” J Steroid Biochem Mol Biol 41(3-8): 747-52.
abstract

Grange, T., J. Roux, G. Rigaud and R. Pictet (1991). “Cell-type specific activity of two glucocorticoid responsive units of rat tyrosine aminotransferase gene is associated with multiple binding sites for C/EBP and a novel liver-specific nuclear factor.” Nucleic Acids Res 19(1): 131-9.
abstract

Rigaud, G., T. Grange and R. Pictet (1991). “Sequenase should be used instead of the Klenow fragment for the synthesis of oligonucleotides labeled to a high specific activity.” Nucleic Acids Res 19(17): 4777.
abstract

Rigaud, G., J. Roux, R. Pictet and T. Grange (1991). “In vivo footprinting of rat TAT gene: dynamic interplay between the glucocorticoid receptor and a liver-specific factor.” Cell 67(5): 977-86.
abstract

Grange, T., J. Roux, M. Fromont-Racine and R. Pictet (1989). “Positive and negative regulation of a transfected chimeric tyrosine aminotransferase gene: effect of copy number.” Exp Cell Res 180(1): 220-33.
abstract

Grange, T., J. Roux, G. Rigaud and R. Pictet (1989). “Two remote glucocorticoid responsive units interact cooperatively to promote glucocorticoid induction of rat tyrosine aminotransferase gene expression.” Nucleic Acids Res 17(21): 8695-709.
abstract

Oddos, J., T. Grange, K. D. Carr, B. Matthews, J. Roux, H. Richard-Foy and R. Pictet (1989). “Nucleotide sequence of 10 kilobases of rat tyrosine aminotransferase gene 5′ flanking region.” Nucleic Acids Res 17(21): 8877-8.
abstract

Grange, T., C. M. de Sa, J. Oddos and R. Pictet (1987). “Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.” Nucleic Acids Res 15(12): 4771-87.
abstract

Rigaud, G., T. Grange and R. Pictet (1987). “The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency.” Nucleic Acids Res 15(2): 857.
abstract

Grange, T., M. Bouloy and M. Girard (1985). “Stable secondary structures at the 3′-end of the genome of yellow fever virus (17 D vaccine strain).” FEBS Lett 188(1): 159-63.
abstract

Grange, T., C. Guenet, J. B. Dietrich, S. Chasserot, M. Fromont, N. Befort, J. Jami, G. Beck and R. Pictet (1985). “Complete complementary DNA of rat tyrosine aminotransferase messenger RNA. Deduction of the primary structure of the enzyme.” J Mol Biol 184(2): 347-50.
abstract

Grange, T., F. Kunst, J. Thillet, B. Ribadeau-Dumas, S. Mousseron, A. Hung, J. Jami and R. Pictet (1984). “Expression of the mouse dihydrofolate reductase cDNA in B. subtilis: a system to select mutant cDNAs coding for methotrexate resistant enzymes.” Nucleic Acids Res 12(8): 3585-601.
abstract

PUBLICATIONS EVA-MARIA GEIGL

Geigl, E.-M. (2021) PCR et paléogénétique : pour le meilleur et pour le pire. PCR and paleogenetics: for best and for worst. Bulletin de l’Académie Nationale de Médecine, 205 (4) :389-395.

 

Leib-Mösch, C., Barton, D., Geigl, E.-M., Brack-Werner, R., Erfle, V., Hehlmann, R., Francke, U.: Two RFLPs associated with the human endogenous retroviral element S71 on chromosome 18q21. Nucl. Acid. Res 17 (6):2367, 1988

 

Hagen, U., Bertram, H., Geigl, E.-M., Kohfeldt, E., Wendel, S.: Radiation-induced clustered damage in DNA. Free Rad. Res. Comm. 6:177-178, 1989

 

Geigl, E.-M., Eckardt-Schupp, F. (1990) Chromosome-specific identification and quantification of S1 nuclease-sensitive sites in yeast chromatin by pulsed field gel electrophoresis. Molecular Microbiology 4(5):801-810.

 

Geigl, E.-M., Eckardt-Schupp, F. (1991) The repair of DNA double-strand breaks and S1 nuclease-sensitive sites can be monitored chromosome-specifically in Saccharomyces cerevisiae using pulsed-field gel electrophoresis. Molecular Microbiology, 5(7):1615-1620.

 

Geigl, E.-M., Eckardt-Schupp, F. (1991) Repair of gamma ray-induced S1 nuclease hypersensitive sites in yeast depends on homologous mitotic recombination and a RAD18-dependent function. Curr. Genet. 20:33-37.

 

Leib-Mösch, C., Haltmeier, M., Werner, T., Geigl, E.-M., Brack-Werner, R., Francke, U., Erfle, V., Hehlmann, R. (1993) Genomic distribution and transcription of solitary HERV-K LTRs. Genomics 18:261-269,

 

Francke, U., Chang, E., Comeau, K., Geigl, E.-M., Giacalone, J., Li, X., Luna, J., Moon, A., Welch, S., Wilgenbus, P. (auteurs listés par ordre alphabétique) (1994) A radiation hybrid map of human chromosome 18. Cytogenet Cell Genet 66:196-213,

 

De Sario, A., Geigl, E.-M., Bernardi, G. (1995) A rapid procedure for the compositional analysis of yeast artificial chromosomes. Nucleic Acids Res. 23(19):4013-4014,

 

De Sario, A., Geigl, E.-M., Palmieri, G., D’Urso, M., Bernardi, G. (J’ai co-encadré l’étudiante en these A. DeSario)A compositional map of human chromosome band Xq28. Proc. Natl. Acad. Sci. USA 93:1298-1302, 1996

 

Geigl, E.-M.: “Inadequate Use of Molecular Hybridization to Analyze DNA in Neanderthal Fossils”. Am. J. Hum. Genetics 68:287-290, 2001 ; S0002-9297(07)62499-9/10.1086/316948

 

Geigl, E.-M. : On the Circumstances Surrounding the Preservation and Analysis of Very Old DNA. Archaeometry 44 (3) :337-342, 2002

 

Geigl, E.-M., Baumer, U., and Koller, J.: New Appproaches to the study of the preservation of biopolymers in fossil bones. Environ. Chem. Letters 2(1) :45-48, 2004

 

Pruvost, M. and Geigl, E.-M. : Real-time Quantitative PCR to Assess the Authenticity of Ancient DNA, Journal of Archaeological Science31(9):1191-1197, 2004

 

Geigl, E.-M. and Pruvost, M. : Plea for a multidisciplinary approach to the study of Neolithic migrations : the analysis of biological witnesses and the input of palaeogenetics. In « Colonisation,, Migration, and Marginal Areas. A Zooarchaeological Approach. » M. Mondini, S. Munoz and S. Wickler (eds), Oxbow Books, p. 10-19, 2004.

 

Geigl, E.-M. : Why ancient DNA research needs taphonomy. In « Biosphere to Lithosphere: new studies in vertebrate taphonomy » T.O’Connor (ed.), Oxbow Books., p. 79-86, 2005.

 

Geigl, E.-M.A personal analysis of the high failure rate of ancient DNA research. Geoarchaeological and Bioarchaeological Studies, Volume 3, p. 463-466, 2005.

 

Pruvost, M., Grange, T., and Geigl, E.-M.: Minimizing DNA-contamination by using UNG-coupled quantitative real-time PCR (UQPCR) on degraded DNA samples: application to ancient DNA studies. Biotechniques 38 :569-575, 2005; DOI 05384ST03

 

Pruvost, M., Schwarz, R., Bessa Correia, V., Champlot, S., Braguier, S., Morel, N., Fernandez-Jalvo, Y., Grange, T. and Geigl, E.-M.: Freshly excavated fossil bones are best for ancient DNA amplification. Proc. Natl. Acad. Sci. USA. 104 (3): 739-744, 2007 ; DOI 0610257104/ 10.1073/pnas.0610257104

 

Pruvost, M., Schwarz, R., Bessa Correia, V., Champlot, S., Grange, T. and Geigl, E.-MDNA diagenesis and palaeogenetic analysis: critical assessment and methodological progress. Palaeogeography, Palaeoclimatology, Palaeoecology, 266 (3-4) : 211-219, 2008doi:10.1016/j.palaeo.2008.03.041

 

Geigl, E.-M. : Palaeogenetics of cattle domestication : Methodological challenge for the study of fossil bones preserved in the domestication center in Southwest Asia. Comptes Rendus Palévol 7(2-3): 99-112, 2008

 

Fernandez-Jalvo, Y., Andrews, P., Pesquero, D., Smith, C., Marin-Monfort, D., Sanchez, B., Geigl, E.-M., Alonso, A. Early bone diagenesis in temperate environments. Part I: Surface features and histology. Palaeogeography, Palaeoclimatology, Palaeoecology 288:62-81, 2010

 

Champlot, S., Berthelot,C., Pruvost, M., Bennett, E.A., Grange, T., Geigl, E.-M. (2010) An Efficient Multistrategy DNA Decontamination Procedure of PCR reagents for Hypersensitive PCR Applications. PLoS ONE 5(9):e13042,. DOI 10.1371/journal.pone.0013042

 

Charruau, P., Fernandes, C., Orozco-Ter Wengel, P. Peters, J., Hunter, L., Ziaie, H., Jourabchian, A., Jowkar, H., Schaller, G., Ostrowski, S., Vercammen, P., Grange, T., Schlötterer, C., Kotze, A. Geigl, E.-M., Walzer, C., Burger, P.A. Phylogeography, genetic structure and population divergence time of cheetahs in Africa and Asia: evidence for long-term geographic isolation. Mol. Ecol. 20(4):706-24, 2010. doi: 10.1111/j.1365-294X.2010.04986

 

Geigl, E.-M. and Grange, T. (2012) Eurasian wild asses in time and space: morphological versus genetic diversity. Annals of Anatomy. 194:88-102,. doi:10.1016/j.aanat.2011.06.002

 

Bennett, E.A., Massilani, D., Lizzo, G., Daligault, J., Geigl, E.-M., Grange, T. (2014) Library construction for ancient genomics: single strand or double strand? Biotechniques 56:289-300. DOI: 10.2144/000114176

 

Nores, C., Morales-Muniz, A., Llorente Rodriguez, L., Bennett, E.A., Geigl, E.-M. (2015) The Iberian zebro: what kind of beast was it? Anthropozoologica 50(1):21-32.

 

Guimaraes, S., Fernandez-Jalvo, Y., Stoetzel, E., Gorgé, O., Bennett, E.A., Denys, C., Grange, T., Geigl, E.-M. (2016) Owl pellets: a wise DNA source for small mammal genetics. Journal of Zoology 298(1):64–74. doi:10.1111/jzo.12285

 

Côté, N.M.L., Daligault, J., Pruvost, M., Bennett, E.A., Gorgé, O., Guimaraes, S., Capelli, N., Le Bailly, M., Geigl*, E.-M., Grange, T.* (2016) A New High-Througput Approach to Genotype Ancient Human Gastrointestinal Parasites.  PLoS One. 2016 11(1):e0146230. doi: 10.1371/journal.pone.0146230. eCollection 2016.

 

Gorgé O, Bennett EA, Massilani D, Daligault J, Pruvost M, Geigl EM, Grange T. (2016) Analysis of Ancient DNA in Microbial Ecology. Methods Mol Biol. 1399:289-315. doi: 10.1007/978-1-4939-3369-3_17.

 

Guimaraes S, Pruvost M, Daligault J, Stoetzel E, Bennett EA, Côté NM, Nicolas V, Lalis A, Denys C, Geigl EM*, Grange T.* (2017) A cost-effective high-throughput metabarcoding approach powerful enough to genotype ~44 000 year-old rodent remains from Northern Africa. Mol Ecol Resour. 17: 405-417. doi: 10.1111/1755-0998.12565

 

Massilani, D., Guimaraes, S., Brugal, J.-P., Bennett, E.A., Tokarska, M., Arbogast, R., Baryshnikov, G., Boeskorov, G., Castel, J.-C., Davydov, S., Madelaine, S., Putelat, O., Spasskaya, N., Uerpmann, H.-P., Grange, T.*, Geigl,E.-M.* (2016) Past climate changes, population dynamics and the origin of Bison in Europe. BMC Biology 14:93-110. DOI 10.1186/s12915-016-0317-7

 

Bennett, E.A., Champlot, S., Peters, J., Arbuckle, B.S., Guimaraes, S., Pruvost, M., Bar-David, S., Davis, S.J.M., Gautier, M., Kaczensky, P., Kuehn, R., Mashkour, M., Morales-Muñiz, M., Pucher, E., Tournepiche, J.-F., Uerpmann, H.-P., Bălăşescu, A., Germonpré, M., Y. Günden, C., Hemami, M.-R., Moullé, P.-E., Öztan, A., Uerpmann, M., Walzer, C., Grange, T.*, Geigl, E.-M.* (2017) Taming the Late Quaternary phylogeography of the Eurasiatic wild ass through ancient and modern DNA. Plos one 12(4): e0174216. /doi.org/10.1371/journal.pone.0174216

 

Ottoni, C., Van Neer, W., De Cupere, B., Daligault, J., Guimaraes, S., Peters, J., Spassov, N., Prendergast, M.E., Boivin, N., Morales-Muniz, A., Bălăşescu, A., Becker, C., Benecke, N., Boronenanț, A., Buitenhuis, H., Chahoud, J., Crowther, A., Llorente, L., Manaseryan, N., Monchot, H., Onar, V., Osypińska, M., Putelat, O., Studer, J., Wierer, U., Decorte, R., Grange, T.*, Geigl, E.-M.* (2017) The paleogenetics of cat dispersal in the ancient world. Nature Ecology & Evolution 1, 0139. DOI: https://doi.org/10.1038/s41559-017-0139.

 

Grange, T., Brugal, J.-P., Flori, L., Gautier, M., Uzunidis, A., and Geigl, E.-M. (2018) The Evolution and Population Diversity of Bison in Pleistocene and Holocene Eurasia: Sex Matters. Diversity 10, 65; doi:10.3390/d10030065

 

Geigl, E.-M., Grange, T. (2018) Ancient DNA: The quest for the best. Mol Ecol Resour. 18:1185–1187. DOI: 10.1111/1755-0998.12931

 

Bennett, E.A., Crevecoeur, I., Viola, B., Derevianko, A.P., Shunkov, M.V., Grange, T., Maureille, B., Geigl, E.-M. (2019) Morphology of the Denisovan phalanx closer to modern humans than to Neandertals. Science Advances, 5:eaaw3950, DOI 10.1126/sciadv.aaw3950, DOI: 10.1126/sciadv.aaw3950

 

Stoetzel, E., Lalis, A., Nicolas, V., Aulagnier,S., Benazzou, T., Dauphin, Y., Abdeljalil El Hajraoui, M., El Hassani, A., Fahd, S., Fekhaoui, M., Geigl, E.-M., Lapointe, F.-J., Leblois, R., Ohler, A., Nespoulet, R., Denys, C.. Quaternary terrestrial microvertebrates from mediterranean northwestern Africa: State-of-the-art focused on recent multidisciplinary studies (2019) Quaternary Science Reviews 224: 105966. DOI: 10.1016/j.quascirev.2019.105966

 

Brunel, S., Bennett, E.A., Cardin, L., Garraud, D., Barrand Emam, H., Beylier, A., Boulestin, B., Chenal, F., Cieselski, E., Convertini, F., Dedet, B., Desenne, S., Dubouloz, J., Duday, H., Fabre, V., Gailledrat, E., Gandelin, M., Gleize, Y., Goepfert, S., Guilaine, J., Hachem, L., Ilett, M., Lambach, F., Mazière, F., Perrin, B., Plouin, S., Pinard, E., Praud, I., Richard, I., Riquier, V., Roure, R., Sendra, B., Thevenet, C., Thiol, S., Vauquelin, E., Vergnaud, L., Grange, T.*, Geigl, E.-M.*, Pruvost, M.* (2020) Ancient genomes from present-day France unveil 7,000 years of its demographic history. Proceedings of the National Academy of Sciences USA, 117(23):12791-12798, DOI:10.1073/pnas.1918034117.

 

Guimaraes, S., Arbuckle, B., Peters, J., Adcock, S., Buitenhuis, H., Chavin, H., Manaseryan, N., Uerpmann, H.-P., Grange, T.*, Geigl, E.-M.* (2020) Ancient DNA shows domestic horses were introduced in the southern Caucasus and Anatolia during the Bronze Age. Science Advances, 6: eabb0030, DOI: 10.1126/sciadv.abb0030

 

Geigl, E.-M. (2021) PCR et paléogénétique : pour le meilleur et pour le pire. Bulletin de l’Académie Nationale de Médecine.

 

CHAPITRES DANS DES OUVRAGES

Eckardt-Schupp, F., Geigl, E.-M., Ahne, F., Siede, W.: Is mismatch repair involved in UV-inducible mutagenesis in yeast?  in: DNA Replication and Mutagenesis. (Eds.: R.E. Moses, W.C. Summers). Washington: ASM, 355-361, 1988

 

Leib-Mösch, C., Bachmann, M., Geigl, E.-M., Brack-Werner, R., Werner, T., Erfle, V., Hehlmann, R.: Expression of S71-related sequences in human cells. Haematol. and Blood Transfusion Vol. 35, p. 256-259 in Modern Trends in Human Leukemia IX, R. Neth et al. (Eds.), Springer Verlag Berlin Heidelberg 1992

 

Geigl, E.-M. and Pruvost, M. : Plea for a multidisciplinary approach to the study of Neolithic migrations : the analysis of biological witnesses and the input of palaeogenetics. In « Colonisation,, Migration, and Marginal Areas. A Zooarchaeological Approach. » M. Mondini, S. Munoz and S. Wickler (eds), Oxbow Books, p. 10-19, 2004.

 

Geigl, E.-M. : Why ancient DNA research needs taphonomy. In « Biosphere to Lithosphere: new studies in vertebrate taphonomy » T.O’Connor (ed.), Oxbow Books., p. 79-86, 2005.

 

Geigl, E.-M.: The domestication of cattle: Insights from a joint archaeozoological-palaeogenetical venture. In “Between Sand and Sea. The Archaeology and Human Ecology of Southwestern Asia”. N.J. Conard, P. Drechsler and A. Morales (eds.), Kerns Verlag Tübingen, p. 69-90, 2011.

 

Geigl, E.-M. Lima de Guimaraes, S., Liesau, C. Palaeogenetic analysis of bovine remains from Camino de las Yeseras and Humanejos. In “Yacimientos Calcoliticos con campaniforme de la region de Madrid: nuevos estudios ». Blasco, C., Liesau, C., and Rios, P. (eds.). Universidad Autonoma de Madrid, Madrid, p. 199-210, 2011.

 

Geigl, E.-M., Champlot, S., de Lima Guimaraes, S., Bennett, E.A., Grange, T. (2013) Molecular Taphonomy of Spy: DNA Preservation in Bone Remains. In Rougier, H. & Semal, P., (eds.) Spy cave. 125 years of multidisciplinary research at the Betche aux Rotches (Jemeppe-sur-Sambre, Province of Namur, Belgium), Volume 1. Anthropologica et Præhistorica, 123/2012. Brussels, Royal Belgian Institute of Natural Sciences, Royal Belgian Society of Anthropology and Praehistory & NESPOS Society, pp. 371-380.

 

Bennett, E.A., Cardin, L., Geigl, E.-M. (2013) Etude paléogénétique des momies d’Antinoé conservées au Musée des Beaux Arts de Lille. In « Les momies d’Antinoé ». Lintz, Y., Coudert, M. (eds.), p. 99-102

 

Geigl, E.-M., and Grange, T. Taphonomie de l’ADN ancien. (2014) In « Manuel de Taphonomie ». C. Denys et M. Patou-Mathis (eds), Editions errance, Collection archéologiques, p. 147-164.

 

Geigl, E.M. (2015) La paléogénétique et paléogénomique. In “Messages d’os”. M. Balasse, J.-P. Brugal, Y. Dauphin, E.-M. Geigl, C. Oberlin, I. Reiche (eds), Editions des Archives contemporaines, p. 429-431

 

Geigl, E.M. and Grange, T. (2015) Les stratégies et enjeux de l’analyse de l’ADN ancien. In “Messages d’os”. M. Balasse, J.-P. Brugal, Y. Dauphin, E.-M. Geigl, C. Oberlin, I. Reiche (eds), Editions des Archives contemporaines, p. 433-452

 

Pruvost,M. and Geigl, E.-M. (2015) « Apport de la paléogénétique à l’étude des processus de domestication animale . In “Messages d’os”. M. Balasse, J.-P. Brugal, Y. Dauphin, E.-M. Geigl, C. Oberlin, I. Reiche (eds), Editions des Archives contemporaines, p. 469-485

 

Geigl, E.M. and Grange, T. (2015) Le génome des lignées humaines archaïques. In “Messages d’os”. M. Balasse, J.-P. Brugal, Y. Dauphin, E.-M. Geigl, C. Oberlin, I. Reiche (eds), Editions des Archives contemporaines, p. 501-520

 

Eva-Maria Geigl, Shirli Bar-David, Albano Beja-Pereira, E. Gus Cothran, Elena Giulotto, Halszka Hrabar, Tsendsuren Oyunsuren, Mélanie Pruvost (2016): Genetics of wild equids. In “Wild Equids: Ecology, Conservation, and Management”, J. Ransom and P. Kazcensky (eds), p. 87-104

 

Geigl, E.-M., Guimaraes, S., Stoetzel, E., Fernandez-Jalvo, Y., Nespoulet, R., Denys, C., Grange, T. (2016) Analyse de la préservation de l’ADN dans les ossements des rongeurs de Témara, Maroc, en remontant dans le temps. In «Approche intégrative de la 6ème extinction : influence de l’installation des hommes modernes au Maroc sur l’évolution de la biodiversité des petits vertébrés terrestres ». Travaux de l’institut Scientifique de l’université Mohammed V de Rabat, Série générale, N° 8, 43-51

 

Geigl, E.-M., Bennett, E.A., Grange, T. (2016) Analyse de la degradation de l’ADN dans une phalange de la Dame du Cavillon. In “La grotte du Cavillon”. H. de Lumley (ed), CNRS Editions, p. 969-975.

 

Bennett, E. A., Gorgé, O., Grange, T., Fernández-Jalvo, Y. and Geigl, E.-M. (2016) Coprolites, Paleogenomics and Bone Content Analysis. In « Azokh Cave and the Transcaucasian Corridor». Yolanda Fernández-Jalvo, Tania King, Levon Yepiskoposyan, and Peter Andrews (eds), Springer, Vertebrate Paleobiology and Paleoanthropology Series, E. Delson and E.J. Sargis (eds), p. 271-286 DOI 10.1007/978-3-319-24924-7 (peer reviewed article)

 

Geigl, E.-M. & Grange, T. (2018) Of Cats and Men: Ancient DNA Reveals How the Cat Conquered the Ancient World. Charlotte Lindqvist and Om P. Rajora (eds.), Paleogenomics, Population Genomics [Om P. Rajora (Editor-in-Chief)], Springer International Publishing AG, part of Springer Nature 2018. DOI https://doi.org/10.1007/13836_2018_26 (peer reviewed article)

 

Geigl, E.-M. & Grange, T. (2019) Using palaeogenetics to unravel the impact of humans on animal populations in the past. In Animals: Cultural Identifiers in Ancient Societies? J. Peters, G. McGlynn & V. Goebel (eds)., Verlag Marie Leidorf (VML) GmbH, Rahden/Westf.  p.131-137

 

Brunel, S., Bennett, E.A., Grange, T., Geigl, E.M., Pruvost, M. (2019) Etude paléogénomique des ossements du dolmen de Saint-Eugène. In Le dolmen de Saint-Eugène : autopsie d’une sépulture collective néolithique. Jean Guilaine., 1 vol., Archives d’écologie préhistorique, Toulouse, France. pp.339-345, 2020, 9782358420266 (pp. 405)

 

PUBLICATIONS DE VULGARISATION DE LA RECHERCHE

1       Geigl, E.-M.L’émergence de la paléogénétique. Biofutur 164:28-34, 1997

3       Geigl, E.-M., Grange, T., Maureille, B. : Le génome néandertalien. Encyclopaedia Universalis, « La Science au présent 2011 », p. 133-140

4       Geigl, E.-M. : Le métier du paléogénéticien. Archéothéma, Hors-série « Les métiers de l’archéologie » 2012, p. 58-59

5       « Mondes polaires – Hommes et biodiversités, des défis pour la science » (2012) R. Chenorkian, M. Raccurt (eds), Cherche-Midi

6       Geigl, E.-M. (2012) Les relations entre les humains et les animaux à la lumière de la génétique.  L’archéologie au laboratoire. Coédition INRAP, Cité des Sciences et de l’Industrie Paris et éditions « La Découverte », p.61-74

7       Geigl, E.-M., Grange, T. (2013) Du potentiel mais des défis méthodologiques à relever. Biofutur 349, 24-27.

8       Geigl, E.-M. (2013) Conserver ou ressusciter les espèces en voie de disparition ? Biofutur 34, 32-34.

9       Pruvost, M., Massilani, D., Geigl, E.-M. (2013) La domestication retracée par l’ADN ancien. Biofutur 349, 36-39.

10     Geigl, E.-M. (2013) Quand étudier le génome des lignées humaines éteintes devient possible. Biofutur 349, 45-49.

11     Geigl, E.-M. (2015) L’apport de la paléogénétique et paléogénomique à l’archéologie. Nouvelles d’Archéologie 138, 10-14.

12     Geigl, E.-M., Bennett, E.A., Grange, T. (2015) Tracing the origin of our species through paleogenomics. BIO Web of Conferences 4, 00005 (2015) DOI: 10.1051/bioconf/20150400005. Owned by the authors, published by EDP Sciences, 2015

13     Geigl, E.-M. and Grange, T. (2016) La paléogénomique, pour une lecture du passé au présent. « L’empreinte du vivant » D. Joly, D. Faure, S. Salamitou (eds)., Editions Cherche Midi, p.151-165.

14     Geigl, E.-M. et Grange, T. (2017) Biodiversité passée et paléogénomique. « Les Big Data à Découvert », Bouzeghoub, M. et Mosseri, R. (eds), CNRS Editions, p. 228 – 229

15     Geigl, E.-M. et Grange, T. (2017) How cats conquered the Ancient world: a 9,000-years DNA tale. The Science Breaker. doi.org/10.25250/thescbr.brk062

16     Geigl, E.-M. (2017) Le peuplement de l’Europe vu par la paléogénomique. In « L’archéologie des migrations », Garcia D. and Le Bras, H. (eds.), La Découverte, Inrap, p. 67 – 80.

17     Geigl, E.-M. et Grange, T. (2017) « Comment le chat a conquis le monde ». Dans « De la Préhistoire à nos jours : Le Chat – Comment il a conquis le monde » Historia, Décembre 2017.

18     Grange, T. et Geigl, E.-M. (2018) « Grâce à la domestication, le chat a gagné le monde ». La Recherche, 531 :65-68

19     Geigl, E.-M. (2018)  L’évolution des mammouths vue par la paléogénomique.  In : Mémoire de Mammouth (244 p): [Exposition Musée national de Préhistoire – Les Eyzies de Tayac 29 juin – 12 novembre 2018] / Commissariat de l’exposition : Catherine Cretin, Stéphane Madelaine. Comité scientifique : Gennady Boeskorov, Peggy Bonnet-Jacquement, Jean-Jacques Cleyet-Merle, Philippe Fosse, Frédéric Plassard. Les Eyzies-de-Tayac, Musée national de Préhistoire. p. 31-34

20     Geigl, E.-M. (2018) La paléogénétique en tant qu’approche archéométrique au cours des 30 dernières années – Paleogenetics as Archeometrical approach over the last 30 years. ArcheoSciences, revue d’archéométrie, 42(1) : 95-104.

21     Grange, T. et Geigl, E.-M. (2019) « Grâce à la domestication, le chat a gagné le monde ». La Recherche – Les Essentiels, n° 30, p. 82-85

22     Geigl, E.-M. & Grange, T. (2019) Changements climatiques : 150 000 ans d’évolution des populations de bisons en Europe. In « 101 sécrets de l’ADN », Faure, D., Joly, D. Salamitou, S. (eds). Editions CNRS, p. 264-266.

23     Geigl, E.-M. (13/01/2020) « Une petite phalange réécrit l’histoire évolutive des humains », The Conversation https://theconversation.com/une-petite-phalange-reecrit-lhistoire-evolutive-des-humains-123156

24     Geigl, E.-M. (2020) « Le Peuplement de la France à la Protohistoire grâce à la paléogénomique ». Archéologia n° 589, 16-17.

25     Geigl, E.-M. (2020) “Qui a habité en France ces 9 000 dernières années ? » The Conversation https://theconversation.com/qui-a-habite-en-france-ces-9-000-dernieres-annees-139769

Thèses de l’équipe depuis 2010:

  • Sophie Champlot : « Colonisation et domestication de l’Europe et du pourtour méditerranéen », Ecole Doctorale « Gènes, Génomes et Cellules », soutenue le 13/7/2010.
  • Nathalie Côté : « Apports de la paléogénétique à l’étude des helminthes garstro-interstinaux anciens». Soutenance de thèse le 16/12/2015 .
  • Olivier Gorgé : «Diagénèse de l’ADN bactérien et analyses métagénomiques de pathologies bactériennes du passé ». Soutenance de thèse le 13/12/2016.
  • Diyendo Massilani : « Paléogénomique des Bovina et de leur domestication ». Soutenance de thèse le 6/7/ 2016.
  • Samantha Brunel : « Analyse paléogénétique du peuplement de la France ». Soutenance de thèse le 14/11/2018.
  • Wejden Bendhafer : « Paléogénomique de l’évolution des Bovina et l’impact sur la domestication des bovins». Soutenance de thèse le 20/12/2021.
  • Oguzhan Parasayan : « Genomic evolution of of ancient French populations » ; en cours depuis Décembre 2018
  • Jeanne Mattei : « Paléogénomique de la domestication des chats » ; en cours depuis Octobre 2019
  • Caitlin Martin : « The evolution of and selection upon the human genome since the invention of agriculture: a historical perspective on diseases”; en cours depuis Octobre 2020

Collaborations scientifiques avec plus de 60 chercheurs nationaux et internationaux de 20 pays, surtout des archéologues, archéozoologues, paléoanthropologues

Financements de l’équipe depuis 2010 :

2021-2024           Projet européen WIDESPREAD-05-2020 – Twinning: « Mapping The Neolithic Expansion In The Mediterranean: A Scientific Collective To Promote Archaeogenomics And Evolutionary Biology Research In Turkey” (H2020-WIDESPREAD-2020-5; CSA 952317; NEOMATRIX

2018-2021           “Paléogénomique de la domestication des bovins pour enrichir les stratégies durables de sélection (PATH2BOS) », ANR, coordinateur : Thierry Grange

2016-2021           “ Etude Génétique de la population Française (FROGH)“, ANR, coordinateur : Christian Dina, Institut du Thorax, UMR 1087/ UMR 6291, Nantes. Equipe « Epigénome & Paléogénome » :  « Paléogénomique de la population médiévale de l’Ouest de la France ».

2015-2019           “Genetic characterization of Ancestral French populations using ancient DNA (ANCESTRA)” ANR JCJC – ANR-15-CE27-0001; Coordinatrice: Mélanie Pruvost, Institut Jacques Monod, Paris.

2014-2016           “The origins of Equid domestication”, National Science Foundation (NSF) Prime Award N° BCS-1311551; Coordinateur: Benjamin Arbuckle, University of Chapel Hill, North Carolina, USA. « Epigénome & Paléogénome » :  “Paleogenetics of the domestication of horses in Anatolia and the Caucasus”

2010-2014           “Influence de l’installation des hommes modernes au Maroc sur l’évolution de la biodiversité des petits vertébrés terrestres (MOHMIE)“ ; Agence Nationale de Recherche (ANR) n° 2009 PEXT 004 05; Coordinatrice : C. Denys, Muséum National d’Histoire Naturelle. « Epigénome & Paléogénome » :  “Paléogénétique des rongeurs au cours des derniers 120,000 ans”

A new study by the Grange/Geigl team published in Science Advances on 14 January 2022!

  • Before the introduction of the domestic horse in Mesopotamia, valuable equids were being harnessed to ceremonial or military four wheeled wagons and used as royal gifts, but their true nature remained unknown.
  • According to a palaeogenetic study, these prestigious animals were the result of a cross between a domestic donkey and a wild ass from Syria, now extinct.
  • This makes them the oldest example of an animal hybrid produced by humans.

> CNRS Press release

 

Communications de notre recherche par le CNRS :

https://archives.cnrs.fr/insb/article/2016/e-m-geigl

https://inee.cnrs.fr/fr/cnrsinfo/ladn-ancien-au-secours-despeces-en-voie-de-disparition-retracer-leur-evolution-pour-mieux

https://lejournal.cnrs.fr/articles/comment-le-chat-a-conquis-le-monde

https://www.cnrs.fr/fr/un-nouveau-morceau-de-phalange-rapproche-plus-les-denisoviens-des-humains-modernes-que-des

https://www.cnrs.fr/fr/7-000-ans-dhistoire-demographique-en-france

https://insb.cnrs.fr/fr/cnrsinfo/ladn-ancien-montre-que-les-chevaux-domestiques-ont-ete-introduits-au-sud-du-caucase-et-en

https://www.cnrs.fr/fr/avant-les-chevaux-des-hybrides-danes-produits-pour-faire-la-guerre