Mitochondria, Metals and Oxidative Stress

Jean-Michel CAMADRO

By studying the assimilation pathways of iron and its intracellular metabolism in a model organism, the yeast Saccharomyces cerevisiae, we can address some of the fundamental problems of biology whilst considering new applications within the field of therapeutics or furthering our knowledge and understanding of the molecular bases of human pathologies.

 

+33 (0)1572780060 / +33 (0)1572780095    jean-michel.camadro(at)ijm.fr     LinkedIn

Iron is an essential trace element for all living cells, but both excess and deficient iron intake are harmful to the cell. Iron homeostasis is therefore tightly regulated in most, if not all, living systems. In humans, diseases related to abnormalities in iron homeostasis can result either from environmental factors or from certain genetic abnormalities.

Iron is the redox center of the heme and iron-sulfur centers, which are essential cofactors in many cellular functions, including energy production and oxygen metabolism. Adequate iron intake is therefore mandatory for normal cell growth and survival. However, due to its chemical reactivity, iron can be toxic to the cell, and paradoxically, iron deficiency and iron overload have been associated with increased production of harmful reactive oxygen species (ROS).

We are studying several aspects of iron metabolism: its transport across the plasma membrane and its utilization in mitochondria, the control of the expression of genes encoding components of the iron metabolism machinery in different eukaryotic model systems. This leads us to study in parallel the control of thiol-dependent responses to oxidative stress, a condition often associated with alterations in iron homeostasis.

Our objects of study are :

– human cells, to better understand the molecular basis of a fatal neurodegenerative disease, Friedreich’s ataxia

– yeasts, S. cerevisiae as a model system, and C. albicans to better understand the role of iron as a virulence factor

– marine microalgae to better understand the mechanisms of adaptation of phytoplankton to major environmental changes: increase in temperature, increase in CO2, change in pH of the marine environment.

We are developing an original and very innovative method of metabolic labelling (SLIM-Labeling) of the different cell types that we study to analyze the quantitative variations of protein abundance in response to different biological stresses. In particular, we apply this new approach to the study of the molecular basis of the yeast/hybrid transition and the epigenetic control of this transition.

Computational biology approaches are essential for the study of the large-scale datasets we produce (transcriptomics, quantitative proteomics, phenomena), and we actively contribute to the production of new open source bioinformatics tools that can be used by the scientific community

 

Research project #1 (Valérie Serre)

Friedreich’s ataxia (FA) is an inherited neurodegenerative disease, due to an unstable GAA repeat located in intron 1 of the FXN gene (9q21.11) coding for frataxin. This protein plays a critical role in the biogenesis of iron-sulfur clusters and iron transport in the mitochondria. A deficiency in this mitochondrial protein leads to the progressive disruption of the central and peripheral nervous systems observed in FA. The size of the shortest allele is inversely proportional to the age of onset of the disease and the time from clinical onset to wheelchair confinement. It is positively correlated with the prevalence of cardiomyopathy.

For many years great efforts have been made to develop treatment to improve the clinical symptoms of FA. However, the development of an effective treatment remains a challenge because the pathophysiology of FA remains not fully resolved. The discovery of biomarkers in rare genetic diseases is accelerating medical research by shedding light on the pathophysiological mechanisms of the diseases. Other than measuring frataxin expression, there are currently no robust protein biomarkers known to be related to FA severity or progression. Furthermore, current knowledge of FA offers various therapeutic approaches under investigation, but there are no reliable biomarkers to assess treatment efficacy.

Our project aims to accelerate the discovery of therapeutic targets using a high-throughput quantitative proteomics approach, with the goal of identifying biomarkers of interest from FA patient plasma samples.

The raw data from the quantitative proteomics are analyzed using statistical and bioinformatics tools according to a pipeline that we have developed. This analysis pipeline uses scripts in R language included in a R Markdown file, allowing to generate detailed, commented, and reproducible analysis reports, as well as the use of open source software platforms. All these bioinformatics analyses follow the general principles of reproducible science with FAIR (Findable, Accessible, Interoperable and Reproducible). The validation of these potential biomarkers can be easily performed by Western blot or ELISA tests in routine.

This work, carried out in close collaboration with the proteomics platform of the Institut Jacques Monod and the Institut du Cerveau (Paris), will hopefully lead to the identification of specific protein markers for the follow-up of AF patients and to the implementation of targeted and efficient therapeutic strategies.

 

Projet de recherche #2 (Françoise Auchère)

Research project #2 (Françoise Auchère)

Regulation of the cellular redox state and adaptive response of the opportunistic pathogen Candida albicans.

Candida albicans is a fungus normally present in oral cavities, skin, vaginal and digestive mucosa. It is a dimorphic yeast that can be found as a yeast or in filamentous form, and the adaptability of this opportunistic pathogen to different environmental conditions plays an important role in the virulence phenomenon.

We are interested in the adaptive metabolic response of C. albicans in the presence of different carbon sources on both yeast and filamentous forms. Using combined biochemical and microbiological approaches, as well as global proteomic approaches, we are investigating the different metabolic adaptations associated with the filamentation process, and the changes in mitochondrial function that may allow adaptation during host tissue invasion.

 

Research project #3 (Emmanuel Lesuisse, deceased in August 2021; project continued in collaboration with Robert Sutack, Biocev Prague)

The bioavailability of metals, and iron in particular, is highly impacted by the modification of marine ecosystems due to human activities (increased CO2 levels and ocean acidification). Surprisingly, phytoplankton metallomes/metalloproteomes are poorly characterized, and predictions on the evolution of iron bioavailability are contradictory. Our goal is to address this data gap and model the effects of ocean acidification on the biogeochemical cycling of iron. A large pool of iron is associated with the surface of different phytoplankton species. We study this iron pool as a function of pCO2 using biochemical and biophysical techniques (sorption studies and synchrotron-based techniques). We characterize the metallomes of species grown under different pCO2 in the presence of different metals and study the effects of metals and pCO2 on interspecific competition. The pH response is also studied in the model alga Ostreococcus tauri.

 

Research project #4 (Jean-Michel Camadro)

New metabolic labeling method for the quantification of (intact) protein abundance variations at the proteome scale.

Our experimental approaches rely heavily on quantitative “label free” proteomic analyses. In collaboration with the engineers of the IJM proteomics platform, we have developed a new analysis method, the “SLIM-labeling”, which uses the ability of most living systems to synthesize the non-essential amino acids that make up proteins from a single carbon source, such as glucose.

SLIM-Labeling aims to increase the level of the light (12C) carbon isotope in proteins, which significantly improves the accuracy of protein mass measurement, and allows the development of novel quantification methods.

We apply this approach to the study of ribosomal proteins abundance in the yeast S. cerevisiae, to the study of the yeast/hyphal transition in C. albicans, and its epigenetic control, to the development of the nematode C. elegans (collaboration with Team Dumont, IJM) and to the labeling of human cells in culture (collaboration with Team Ladoux/ Mège, IJM)

 

Transversal research project (Pierre Poulain)

Development of methods and tools for the exploration, analysis and visualization of biological data, particularly in proteomics.

 

Among these methods and tools developped in the group, we can mention :

– The AutoClassWrapper Python library and the AutoClassWeb web service for unsupervised Bayesian classification of omics experimental results.

– The bSLIM method for quantitative mass spectrometry analysis by metabolic labeling with a light isotope.

– Finally, the collaborative project Minomics aims at multi-omics analysis of biological networks by a holistic approach.

 

Pierre Poulain is also a Software Heritage Ambassador to promote the archiving of open source scientific software.

Group Leader :

Jean-Michel CAMADRO
Téléphone : +33 (0)157278029 

jean-michel.camadro(at)ijm.fr

 

Members:

  • Françoise AUCHERE (Enseignant chercheur)
    Téléphone : +33 (0)157278028
  • Thibaut POINSIGNON (Doctorant)
  • Pierre POULAIN (Enseignant chercheur)
    Téléphone : +33 (0)157278028
  • Nicolas SENECAUT (Doctorant)
    Téléphone : +33 (0)157278028
  • Valérie SERRE (Enseignant chercheur)
    Téléphone : +33 (0)157278028
  • Robert SUTAK (Biocev, Prague, visiting scientist)
  • Léa LEUTHY (stagiaire de recherche)

1: Functional networks of co-expressed genes to explore iron homeostasis processes in the pathogenic yeast Candida glabrata.

Denecker T, Zhou Li Y, Fairhead C, Budin K, Camadro JM, Bolotin-Fukuhara M, Angoulvant A, Lelandais G.

NAR Genom Bioinform. 2020 Apr 20;2(2):lqaa027. doi: 10.1093/nargab/lqaa027.

PMID: 33575583; PMCID: PMC7671338.

 

2: Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy.

Sénécaut N, Alves G, Weisser H, Lignières L, Terrier S, Yang-Crosson L, Poulain P, Lelandais G, Yu YK, Camadro JM.

J Proteome Res. 2021 Mar 5;20(3):1476-1487.

doi: 10.1021/acs.jproteome.0c00478. Epub 2021 Feb 11.

PMID: 33573382; PMCID: PMC8459934.

 

3: Iron Uptake Mechanisms in Marine Phytoplankton.

Sutak R, Camadro JM, Lesuisse E.

Front Microbiol. 2020 Nov 5;11:566691. doi:

10.3389/fmicb.2020.566691.

PMID: 33250865; PMCID: PMC7676907.

 

4: The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans.

Duval C, Macabiou C, Garcia C, Lesuisse E, Camadro JM, Auchère F.

Microbiologyopen. 2020 Feb;9(2):e970. doi:

10.1002/mbo3.970. Epub 2019 Dec 1.

PMID: 31788966; PMCID: PMC7002100.

 

5: Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death.

El Banna N, Hatem E, Heneman-Masurel A, Léger T, Baïlle D, Vernis L, Garcia C, Martineau S, Dupuy C, Vagner S, Camadro JM, Huang ME.

Redox Biol. 2019 Sep;26:101290. doi: 10.1016/j.redox.2019.101290. Epub 2019 Aug 2.

PMID: 31412312; PMCID: PMC6831881.

 

6: NDUFS6 related Leigh syndrome: a case report and review of the literature.

Rouzier C, Chaussenot A, Fragaki K, Serre V, Ait-El-Mkadem S, Richelme C, Paquis-Flucklinger V, Bannwarth S.

J Hum Genet. 2019 Jul;64(7):637-645. doi: 10.1038/s10038-019-0594-4. Epub 2019 Apr 4.

PMID: 30948790.

 

7: N-homocysteinylation of tau and MAP1 is increased in autopsy specimens of Alzheimer’s disease and vascular dementia.

Bossenmeyer-Pourié C, Smith AD, Lehmann S, Deramecourt V, Sablonnière B, Camadro JM, Pourié G, Kerek R, Helle D, Umoret R, Guéant-Rodriguez RM, Rigau V, Gabelle A, Sequeira JM, Quadros EV, Daval JL, Guéant JL.

J Pathol. 2019 Jul;248(3):291-303. doi: 10.1002/path.5254. Epub 2019 Mar 19.

PMID: 30734924.

 

8: Quantitative proteomics in Friedreich’s ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis.

Télot L, Rousseau E, Lesuisse E, Garcia C, Morlet B, Léger T, Camadro JM, Serre V.

Biochim Biophys Acta Mol Basis Dis. 2018 Apr;1864(4 Pt

A):997-1009. doi: 10.1016/j.bbadis.2018.01.010. Epub 2018 Jan 9.

PMID: 29329987.

2022

Additional insights into the organization of transcriptional regulatory modules based on a 3D model of the Saccharomyces cerevisiae genome.

Poinsignon T, Gallopin M, Camadro JM, Poulain P, Lelandais G.

BMC Res Notes. 2022 Feb 19;15(1):67. doi: 10.1186/s13104-022-05940-5.

PMID: 35183229

 

2021

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii.

Bertini EV, Torres MA, Léger T, Garcia C, Hong KW, Chong TM, Castellanos de Figueroa LI, Chan KG, Dessaux Y, Camadro JM, Nieto-Peñalver CG.

Genomics. 2021 Nov;113(6):4352-4360. doi: 10.1016/j.ygeno.2021.11.017. Epub 2021 Nov 15.

PMID: 34793950

 

Fetal Programming by Methyl Donor Deficiency Produces Pathological Remodeling of the Ascending Aorta.

Balint B, Hergalant S, Camadro JM, Blaise S, Vanalderwiert L, Lignières L, Guéant-Rodriguez RM, Guéant JL.

Arterioscler Thromb Vasc Biol. 2021 Jun;41(6):1928-1941. doi: 10.1161/ATVBAHA.120.315587. Epub 2021 Apr 8. PMID: 33827257

 

Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy.

Sénécaut N, Alves G, Weisser H, Lignières L, Terrier S, Yang-Crosson LPoulain P, Lelandais G, Yu YK, Camadro JM.

J Proteome Res. 2021 Mar 5;20(3):1476-1487. doi: 10.1021/acs.jproteome.0c00478. Epub 2021 Feb 11. PMID: 33573382

 

Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability.

Kotabova E, Malych R, Pierella Karlusich JJ, Kazamia E, Eichner M, Mach J, Lesuisse E, Bowler C, Prášil O, Sutak R.

mSystems. 2021 Feb 9;6(1):e00738-20. doi: 10.1128/mSystems.00738-20. PMID: 33563784

 

2020

 

Iron Uptake Mechanisms in Marine Phytoplankton.

Sutak R, Camadro JMLesuisse E.

Front Microbiol. 2020 Nov 5;11:566691. doi: 10.3389/fmicb.2020.566691. eCollection 2020. PMID: 33250865

 

Bi-allelic Variations of SMO in Humans Cause a Broad Spectrum of Developmental Anomalies Due to Abnormal Hedgehog Signaling.

Le TL, Sribudiani Y, Dong X, Huber C, Kois C, Baujat G, Gordon CT, Mayne V, Galmiche L, Serre V, Goudin N, Zarhrate M, Bole-Feysot C, Masson C, Nitschké P, Verheijen FW, Pais L, Pelet A, Sadedin S, Pugh JA, Shur N, White SM, El Chehadeh S, Christodoulou J, Cormier-Daire V, Hofstra RMW, Lyonnet S, Tan TY, Attié-Bitach T, Kerstjens-Frederikse WS, Amiel J, Thomas S.

Am J Hum Genet. 2020 Jun 4;106(6):779-792. doi: 10.1016/j.ajhg.2020.04.010. Epub 2020 May 14. PMID: 32413283

 

Functional networks of co-expressed genes to explore iron homeostasis processes in the pathogenic yeast Candida glabrata.

Denecker T, Zhou Li Y, Fairhead C, Budin K, Camadro JM, Bolotin-Fukuhara M, Angoulvant A, Lelandais G.

NAR Genom Bioinform. 2020 Apr 20;2(2):lqaa027. doi: 10.1093/nargab/lqaa027. eCollection 2020 Jun. PMID: 33575583

 

Analysis of fibroblasts from patients with cblC and cblG genetic defects of cobalamin metabolism reveals global dysregulation of alternative splicing.

Rashka C, Hergalant S, Dreumont N, Oussalah A, Camadro JM, Marchand V, Hassan Z, Baumgartner MR, Rosenblatt DS, Feillet F, Guéant JL, Flayac J, Coelho D.

Hum Mol Genet. 2020 Jul 29;29(12):1969-1985. doi: 10.1093/hmg/ddaa027. PMID: 32068834

 

The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans.

Duval CMacabiou C, Garcia C, Lesuisse ECamadro JMAuchère F.

Microbiologyopen. 2020 Feb;9(2):e970. doi: 10.1002/mbo3.970. Epub 2019 Dec 1. PMID: 31788966

 

2019

 

A novel variant m.8561C>T in the overlapping region of MT-ATP6 and MT-ATP8 in a child with early-onset severe neurological signs.

Fragaki K, Chaussenot A, Serre V, Acquaviva C, Bannwarth S, Rouzier C, Chabrol B, Paquis-Flucklinger V.

Mol Genet Metab Rep. 2019 Nov 21;21:100543. doi: 10.1016/j.ymgmr.2019.100543. eCollection 2019 Dec. PMID: 31788426

 

Copper and iron metabolism in Ostreococcus tauri – the role of phytotransferrin, plastocyanin and a chloroplast copper-transporting ATPase.

Scheiber IF, Pilátová J, Malych R, Kotabova E, Krijt M, Vyoral D, Mach J, Léger T, Camadro JM, Prášil O, Lesuisse E, Sutak R.

Metallomics. 2019 Oct 16;11(10):1657-1666. doi: 10.1039/c9mt00078j. PMID: 31380866

 

LGP2 binds to PACT to regulate RIG-I- and MDA5-mediated antiviral responses.

Sanchez David RY, Combredet C, Najburg V, Millot GA, Beauclair G, Schwikowski B, Léger T, Camadro JM, Jacob Y, Bellalou J, Jouvenet N, Tangy F, Komarova AV.

Sci Signal. 2019 Oct 1;12(601):eaar3993. doi: 10.1126/scisignal.aar3993. PMID: 31575732

 

Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death.

El Banna N, Hatem E, Heneman-Masurel A, Léger T, Baïlle D, Vernis L, Garcia C, Martineau S, Dupuy C, Vagner S, Camadro JM, Huang ME.

Redox Biol. 2019 Sep;26:101290. doi: 10.1016/j.redox.2019.101290. Epub 2019 Aug 2. PMID: 31412312

 

Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to assess data reproducibility.

Lelandais G, Denecker T, Garcia C, Danila N, Léger T, Camadro JM.

BMC Res Notes. 2019 Aug 1;12(1):470. doi: 10.1186/s13104-019-4505-8. PMID: 31370875

 

NDUFS6 related Leigh syndrome: a case report and review of the literature.

Rouzier C, Chaussenot A, Fragaki K, Serre V, Ait-El-Mkadem S, Richelme C, Paquis-Flucklinger V, Bannwarth S.

J Hum Genet. 2019 Jul;64(7):637-645. doi: 10.1038/s10038-019-0594-4. Epub 2019 Apr 4. PMID: 30948790

 

AutoClassWrapper: a Python wrapper for AutoClass C classification

Camadro JM & Poulain P

The Journal of Open Source Software 2019, accepted July 25; DOI: 10.21105/joss.01390

 

In Vitro interaction between yeast frataxin and superoxide dismutases: Influence of mitochondrial metals.

Han THL, Camadro JM, Barbault F, Santos R, El Hage Chahine JM, Ha-Duong NT.

Biochim Biophys Acta Gen Subj. 2019 May;1863(5):883-892. doi: 10.1016/j.bbagen.2019.02.011. Epub 2019 Feb 21. PMID: 30797804

 

Adsorption of Proteins on m-CPPD and Urate Crystals Inhibits Crystal-induced Cell Responses: Study on Albumin-crystal Interaction.

Renaudin F, Sarda S, Campillo-Gimenez L, Séverac C, Léger T, Charvillat C, Rey C, Lioté F, Camadro JM, Ea HK, Combes C.

J Funct Biomater. 2019 Apr 25;10(2):18. doi: 10.3390/jfb10020018. PMID: 31027151

 

Pixel: a content management platform for quantitative omics data.

Denecker T, Durand W, Maupetit J, Hébert C, Camadro JMPoulain P, Lelandais G.

PeerJ. 2019 Mar 27;7:e6623. doi: 10.7717/peerj.6623. eCollection 2019. PMID: 30944779

 

N-homocysteinylation of tau and MAP1 is increased in autopsy specimens of Alzheimer’s disease and vascular dementia.

Bossenmeyer-Pourié C, Smith AD, Lehmann S, Deramecourt V, Sablonnière B, Camadro JM, Pourié G, Kerek R, Helle D, Umoret R, Guéant-Rodriguez RM, Rigau V, Gabelle A, Sequeira JM, Quadros EV, Daval JL, Guéant JL.

J Pathol. 2019 Jul;248(3):291-303. doi: 10.1002/path.5254. Epub 2019 Mar 19. PMID: 30734924

 

Proteomic Landscape of Cholangiocarcinomas Reveals Three Different Subgroups According to Their Localization and the Aspect of Non-Tumor Liver.

Le Faouder J, Gigante E, Léger T, Albuquerque M, Beaufrère A, Soubrane O, Dokmak S, Camadro JM, Cros J, Paradis V.

Proteomics Clin Appl. 2019 Jan;13(1):e1800128. doi: 10.1002/prca.201800128. Epub 2018 Dec 19. PMID: 30520266

 

2018

 

AAV-8 and AAV-9 Vectors Cooperate with Serum Proteins Differently Than AAV-1 and AAV-6.

Denard J, Rouillon J, Leger T, Garcia C, Lambert MP, Griffith G, Jenny C, Camadro JM, Garcia L, Svinartchouk F.

Mol Ther Methods Clin Dev. 2018 Aug 8;10:291-302. doi: 10.1016/j.omtm.2018.08.001. eCollection 2018 Sep 21. PMID: 30155509

 

Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms.

Kazamia E, Sutak R, Paz-Yepes J, Dorrell RG, Vieira FRJ, Mach J, Morrissey J, Leon S, Lam F, Pelletier E, Camadro JM, Bowler C, Lesuisse E.

Sci Adv. 2018 May 16;4(5):eaar4536. doi: 10.1126/sciadv.aar4536. eCollection 2018 May. PMID: 29774236

 

Quantitative proteomics in Friedreich’s ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis.

Télot L, Rousseau E, Lesuisse E, Garcia C, Morlet B, Léger T, Camadro JMSerre V.

Biochim Biophys Acta Mol Basis Dis. 2018 Apr;1864(4 Pt A):997-1009. doi: 10.1016/j.bbadis.2018.01.010. Epub 2018 Jan 9. PMID: 29329987

 

Meet-U: Educating through research immersion.

Abdollahi N, Albani A, Anthony E, Baud A, Cardon M, Clerc R, Czernecki D, Conte R, David L, Delaune A, Djerroud S, Fourgoux P, Guiglielmoni N, Laurentie J, Lehmann N, Lochard C, Montagne R, Myrodia V, Opuu V, Parey E, Polit L, Privé S, Quignot C, Ruiz-Cuevas M, Sissoko M, Sompairac N, Vallerix A, Verrecchia V, Delarue M, Guérois R, Ponty Y, Sacquin-Mora S, Carbone A, Froidevaux C, Le Crom S, Lespinet O, Weigt M, Abboud S, Bernardes J, Bouvier G, Dequeker C, Ferré A, Fuchs P, Lelandais G, Poulain P, Richard H, Schweke H, Laine E, Lopes A.

PLoS Comput Biol. 2018 Mar 15;14(3):e1005992. doi: 10.1371/journal.pcbi.1005992. eCollection 2018 Mar. PMID: 29543809

 

Novel de novo ZBTB20 mutations in three cases with Primrose syndrome and constant corpus callosum anomalies.

Alby C, Boutaud L, Bessières B, Serre V, Rio M, Cormier-Daire V, de Oliveira J, Ichkou A, Mouthon L, Gordon CT, Bonnière M, Mechler C, Nitschke P, Bole C, Lyonnet S, Bahi-Buisson N, Boddaert N, Colleaux L, Roth P, Ville Y, Vekemans M, Encha-Razavi F, Attié-Bitach T, Thomas S.

Am J Med Genet A. 2018 May;176(5):1091-1098. doi: 10.1002/ajmg.a.38684. PMID: 29681083

 

Impaired Transferrin Receptor Palmitoylation and Recycling in Neurodegeneration with Brain Iron Accumulation.

Drecourt A, Babdor J, Dussiot M, Petit F, Goudin N, Garfa-Traoré M, Habarou F, Bole-Feysot C, Nitschké P, Ottolenghi C, Metodiev MD, Serre V, Desguerre I, Boddaert N, Hermine O, Munnich A, Rötig A.

Am J Hum Genet. 2018 Feb 1;102(2):266-277. doi: 10.1016/j.ajhg.2018.01.003. PMID: 29395073

 

2017

 

A Simple Light Isotope Metabolic Labeling (SLIM-labeling) Strategy: A Powerful Tool to Address the Dynamics of Proteome Variations In Vivo.

Léger T, Garcia C, Collomb L, Camadro JM.

Mol Cell Proteomics. 2017 Nov;16(11):2017-2031. doi: 10.1074/mcp.M117.066936. Epub 2017 Aug 18. PMID: 28821603

 

Mechanisms of iron and copper-frataxin interactions.

Han THL, Camadro JM, Santos R, Lesuisse E, El Hage Chahine JM, Ha-Duong NT.

Metallomics. 2017 Aug 16;9(8):1073-1085. doi: 10.1039/c7mt00031f. PMID: 28573291

 

Dysfunction of mitochondrial Lon protease and identification of oxidized protein in mouse brain following exposure to MPTP: Implications for Parkinson disease.

Bulteau AL, Mena NP, Auchère F, Lee I, Prigent A, Lobsiger CS, Camadro JM, Hirsch EC.

Free Radic Biol Med. 2017 Jul;108:236-246. doi: 10.1016/j.freeradbiomed.2017.03.036. Epub 2017 Mar 30. PMID: 28365360

 

Acclimation of a low iron adapted Ostreococcus strain to iron limitation through cell biomass lowering.

Botebol H, Lelandais G, Six C, Lesuisse E, Meng A, Bittner L, Lecrom S, Sutak R, Lozano JC, Schatt P, Vergé V, Blain S, Bouget FY.

Sci Rep. 2017 Mar 23;7(1):327. doi: 10.1038/s41598-017-00216-6. PMID: 28336917

 

Modulation of the specific glutathionylation of mitochondrial proteins in the yeast Saccharomyces cerevisiae under basal and stress conditions.

Gergondey R, Garcia C, Marchand CH, Lemaire SD, Camadro JMAuchère F.

Biochem J. 2017 Mar 15;474(7):1175-1193. doi: 10.1042/BCJ20160927. PMID: 28167699

 

A novel CISD2 mutation associated with a classical Wolfram syndrome phenotype alters Ca2+ homeostasis and ER-mitochondria interactions.

Rouzier C, Moore D, Delorme C, Lacas-Gervais S, Ait-El-Mkadem S, Fragaki K, Burté F, Serre V, Bannwarth S, Chaussenot A, Catala M, Yu-Wai-Man P, Paquis-Flucklinger V.

Hum Mol Genet. 2017 May 1;26(9):1786. doi: 10.1093/hmg/ddx130. PMID: 28475771

 

A novel CISD2 mutation associated with a classical Wolfram syndrome phenotype alters Ca2+ homeostasis and ER-mitochondria interactions.

Rouzier C, Moore D, Delorme C, Lacas-Gervais S, Ait-El-Mkadem S, Fragaki K, Burté F, Serre V, Bannwarth S, Chaussenot A, Catala M, Yu-Wai-Man P, Paquis-Flucklinger V.

Hum Mol Genet. 2017 May 1;26(9):1599-1611. doi: 10.1093/hmg/ddx060. PMID: 28335035

 

Compound heterozygosity for severe and hypomorphic NDUFS2 mutations cause non-syndromic LHON-like optic neuropathy.

Gerber S, Ding MG, Gérard X, Zwicker K, Zanlonghi X, Rio M, Serre V, Hanein S, Munnich A, Rotig A, Bianchi L, Amati-Bonneau P, Elpeleg O, Kaplan J, Brandt U, Rozet JM.

J Med Genet. 2017 May;54(5):346-356. doi: 10.1136/jmedgenet-2016-104212. Epub 2016 Dec 28. PMID: 28031252

 

Mutations in MDH2, Encoding a Krebs Cycle Enzyme, Cause Early-Onset Severe Encephalopathy.

Ait-El-Mkadem S, Dayem-Quere M, Gusic M, Chaussenot A, Bannwarth S, François B, Genin EC, Fragaki K, Volker-Touw CLM, Vasnier C, Serre V, van Gassen KLI, Lespinasse F, Richter S, Eisenhofer G, Rouzier C, Mochel F, De Saint-Martin A, Abi Warde MT, de Sain-van der Velde MGM, Jans JJM, Amiel J, Avsec Z, Mertes C, Haack TB, Strom T, Meitinger T, Bonnen PE, Taylor RW, Gagneur J, van Hasselt PM, Rötig A, Delahodde A, Prokisch H, Fuchs SA, Paquis-Flucklinger V.

Am J Hum Genet. 2017 Jan 5;100(1):151-159. doi: 10.1016/j.ajhg.2016.11.014. Epub 2016 Dec 15. PMID: 27989324

 

2016

 

Ostreococcus tauri is a new model green alga for studying iron metabolism in eukaryotic phytoplankton.

Lelandais G, Scheiber I, Paz-Yepes J, Lozano JC, Botebol H, Pilátová J, Žárský V, Léger T, Blaiseau PL, Bowler C, Bouget FY, Camadro JM, Sutak R, Lesuisse E.

BMC Genomics. 2016 May 3;17:319. doi: 10.1186/s12864-016-2666-6. PMID: 27142620

 

The adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans.

Gergondey R, Garcia C, Serre VCamadro JMAuchère F.

Biochim Biophys Acta. 2016 Jul;1862(7):1309-23. doi: 10.1016/j.bbadis.2016.04.004. Epub 2016 Apr 13. PMID: 27083931

 

Reply: High prevalence of CHCHD10 mutations in patients with frontotemporal dementia from China.

Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore D, Verschueren A, Rouzier C, Le Ber I, Augé G, Cochaud C, Lespinasse F, N’Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V.

Brain. 2016 Apr;139(Pt 4):e22. doi: 10.1093/brain/awv368. Epub 2015 Dec 30. PMID: 26719380

 

Insights into Brain Glycogen Metabolism: THE STRUCTURE OF HUMAN BRAIN GLYCOGEN PHOSPHORYLASE.

Mathieu C, Li de la Sierra-Gallay I, Duval R, Xu X, Cocaign A, Léger T, Woffendin G, Camadro JM, Etchebest C, Haouz A, Dupret JM, Rodrigues-Lima F.

J Biol Chem. 2016 Aug 26;291(35):18072-83. doi: 10.1074/jbc.M116.738898. Epub 2016 Jul 8. PMID: 27402852

 

A Network of Paralogous Stress Response Transcription Factors in the Human Pathogen Candida glabrata.

Merhej J, Thiebaut A, Blugeon C, Pouch J, Ali Chaouche Mel A, Camadro JM, Le Crom S, Lelandais G, Devaux F.

Front Microbiol. 2016 May 9;7:645. doi: 10.3389/fmicb.2016.00645. eCollection 2016. PMID: 27242683

 

The Metacaspase (Mca1p) Restricts O-glycosylation During Farnesol-induced Apoptosis in Candida albicans.

Léger T, Garcia C, Camadro JM.

Mol Cell Proteomics. 2016 Jul;15(7):2308-23. doi: 10.1074/mcp.M116.059378. Epub 2016 Apr 28. PMID: 27125826

 

Label-Free Quantitative Proteomics in Yeast.

Léger T, Garcia C, Videlier M, Camadro JM.

Methods Mol Biol. 2016;1361:289-307. doi: 10.1007/978-1-4939-3079-1_16. PMID: 26483028

 

2015

 

Reply: Is CHCHD10 Pro34Ser pathogenic for frontotemporal dementia and amyotrophic lateral sclerosis?

Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore D, Verschueren A, Rouzier C, Le Ber I, Augé G, Cochaud C, Lespinasse F, N’Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V.

Brain. 2015 Oct;138(Pt 10):e386. doi: 10.1093/brain/awv116. Epub 2015 May 7. PMID: 25953779

 

Reply: A distinct clinical phenotype in a German kindred with motor neuron disease carrying a CHCHD10 mutation.

Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore D, Verschueren A, Rouzier C, Le Ber I, Augé G, Cochaud C, Lespinasse F, N’Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V.

Brain. 2015 Sep;138(Pt 9):e377. doi: 10.1093/brain/awv015. Epub 2015 Feb 12. PMID: 25681413

 

Reply: CHCHD10 mutations in Italian patients with sporadic amyotrophic lateral sclerosis.

Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore D, Verschueren A, Rouzier C, Le Ber I, Augé G, Cochaud C, Lespinasse F, N’Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V.

Brain. 2015 Aug;138(Pt 8):e373. doi: 10.1093/brain/awu385. Epub 2015 Jan 8. PMID: 25576309

 

Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton.

Botebol H, Lesuisse E, Šuták R, Six C, Lozano JC, Schatt P, Vergé V, Kirilovsky A, Morrissey J, Léger T, Camadro JM, Gueneugues A, Bowler C, Blain S, Bouget FY.

Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14652-7. doi: 10.1073/pnas.1506074112. Epub 2015 Nov 9. PMID: 26553998

 

A Yeast/Drosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency.

Seguin A, Monnier V, Palandri A, Bihel F, Rera M, Schmitt M, Camadro JM, Tricoire H, Lesuisse E.

Oxid Med Cell Longev. 2015;2015:565140. doi: 10.1155/2015/565140. Epub 2015 Oct 11. PMID: 26523199

 

A novel protein, ubiquitous in marine phytoplankton, concentrates iron at the cell surface and facilitates uptake.

Morrissey J, Sutak R, Paz-Yepes J, Tanaka A, Moustafa A, Veluchamy A, Thomas Y, Botebol H, Bouget FY, McQuaid JB, Tirichine L, Allen AE, Lesuisse E, Bowler C.

Curr Biol. 2015 Feb 2;25(3):364-371. doi: 10.1016/j.cub.2014.12.004. Epub 2014 Dec 31. PMID: 25557662

 

The metacaspase (Mca1p) has a dual role in farnesol-induced apoptosis in Candida albicans.

Léger T, Garcia C, Ounissi M, Lelandais G, Camadro JM.

Mol Cell Proteomics. 2015 Jan;14(1):93-108. doi: 10.1074/mcp.M114.041210. Epub 2014 Oct 27. PMID: 25348831

 

Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies.

Rouillon J, Poupiot J, Zocevic A, Amor F, Léger T, Garcia C, Camadro JM, Wong B, Pinilla R, Cosette J, Coenen-Stass AM, Mcclorey G, Roberts TC, Wood MJ, Servais L, Udd B, Voit T, Richard I, Svinartchouk F.

Hum Mol Genet. 2015 Sep 1;24(17):4916-32. doi: 10.1093/hmg/ddv214. Epub 2015 Jun 9. PMID: 26060189

 

Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity.

Thomas S, Cantagrel V, Mariani L, Serre V, Lee JE, Elkhartoufi N, de Lonlay P, Desguerre I, Munnich A, Boddaert N, Lyonnet S, Vekemans M, Lisgo SN, Caspary T, Gleeson J, Attié-Bitach T.

Eur J Hum Genet. 2015 May;23(5):621-7. doi: 10.1038/ejhg.2014.156. Epub 2014 Aug 20. PMID: 25138100

Nicolas Sénécaut, Université Paris Cité

Directeur de thèse : Jean-Michel Camadro (IJM) ; co-DT : Gaëlle Lelandais

 

Thibaut Poinsignon, Université Paris Saclay

Directrice de thèse : Gaëlle Lelandais, co-DT : Pierre Poulain (IJM)

 

Katerina Zeniskova, Biocev, Prague, République Tchèque

Directeur de thèse : Robert Sutak ; co-DT : Jean-Michel Camadro (IJM)

Pr Gaëlle Lelandais, I2BC, Université Paris Saclay

 

Dr Yi-Kuo Yu, NCBI, NIH, Bethesda, USA

 

Pr Denis Mestivier, UPEC

 

Dr Stéphane Lemaire, IBPS, Sorbonne Université

 

Pr Jean-Louis Guéant, INSERM, Université de Lorraine

 

Dr Robert Sutac, Biocev, Prague, Rep. Tchèque

 

Pr Pavel Martasek, 1st Faculty of Medicine, Charles University, Prague, Rep. Tchèque

Data mining, Visualization and Computational Modeling of Redox Signaling Networks – MinOmics

2020-03 to 2024-03 | Grant

National Agency for Research (Paris, FR)

URL: https://app.dimensions.ai/details/grant/grant.9466085

GRANT_NUMBER: ANR-19-CE45-0017

 

Microbial Communities in Biomedical and Environmental Areas, and Systems Biology

2018-09 to 2022-11 | Grant

European Commission (Brussels, BE)

URL: https://app.dimensions.ai/details/grant/grant.7819194

GRANT_NUMBER: 810224

 

High performance MS-based proteomics by reducing stable isotopes complexity in vivo – SLIM-labeling

2018-09 to 2022-03 | Grant

National Agency for Research (Paris, FR)

URL: https://app.dimensions.ai/details/grant/grant.8639705

GRANT_NUMBER: ANR-18-CE44-0014

 

Le protéome urinaire dans l’ataxie de Friedreich : apport de la protéomique quantitative pour la découverte de biomarqueurs 2019-2019-2022 | Grant

Association Française Ataxie de Friedreich (AFAF, Paris FR)

URL : www.afaf.asso.fr