Mécanotransduction :

de la surface de la cellule à son noyau

 

 

Notre équipe étudie comment nos cellules réagissent à leur environnement mécanique. Nous proposons une approche par microscopies de fluorescence quantitatives et ingénierie génétique pour des réponses de la molécule au tissu.

 

Responsable : Nicolas Borghi

Directeur de Recherche CNRS

+33 (0)157278041

nicolas.borghi(at)ijm.fr

 

 

RECHERCHEMEMBRESPUBLISNEWSEMPLOI

 

Thèmes et Axes de recherche

 

Biologie quantitative et modélisation , Dynamique cellulaire et signalisation , Pathologies moléculaires et cellulaires

 

Sélection de publications

 

1: Déjardin T, Carollo PS, Sipieter F, Davidson PM, Seiler C, Cuvelier D, Cadot

B, Sykes C, Gomes ER, Borghi N. Nesprins are mechanotransducers that

discriminate epithelial-mesenchymal transition programs. J Cell Biol. 2020 Oct

5;219(10):e201908036. doi: 10.1083/jcb.201908036. PMID: 32790861; PMCID:

PMC7659719.

 

2: Davidson PM, Battistella A, Déjardin T, Betz T, Plastino J, Borghi N, Cadot

B, Sykes C. Nesprin-2 accumulates at the front of the nucleus during confined

cell migration. EMBO Rep. 2020 Jul 3;21(7):e49910. doi: 10.15252/embr.201949910.

Epub 2020 May 17. PMID: 32419336; PMCID: PMC7332974.

 

3: Audugé N, Padilla-Parra S, Tramier M, Borghi N, Coppey-Moisan M. Chromatin

condensation fluctuations rather than steady-state predict chromatin

accessibility. Nucleic Acids Res. 2019 Jul 9;47(12):6184-6194. doi:

10.1093/nar/gkz373. PMID: 31081027; PMCID: PMC6614833.

 

4: De Pascalis C, Pérez-González C, Seetharaman S, Boëda B, Vianay B, Burute M,

Leduc C, Borghi N, Trepat X, Etienne-Manneville S. Intermediate filaments

control collective migration by restricting traction forces and sustaining cell-

cell contacts. J Cell Biol. 2018 Sep 3;217(9):3031-3044. doi:

10.1083/jcb.201801162. Epub 2018 Jul 6. PMID: 29980627; PMCID: PMC6122997.

 

5: Gayrard C, Bernaudin C, Déjardin T, Seiler C, Borghi N. Src- and confinement-

dependent FAK activation causes E-cadherin relaxation and β-catenin activity. J

Cell Biol. 2018 Mar 5;217(3):1063-1077. doi: 10.1083/jcb.201706013. Epub 2018

Jan 8. PMID: 29311227; PMCID: PMC5839785.

 

6: Sarangi BR, Gupta M, Doss BL, Tissot N, Lam F, Mège RM, Borghi N, Ladoux B.

Coordination between Intra- and Extracellular Forces Regulates Focal Adhesion

Dynamics. Nano Lett. 2017 Jan 11;17(1):399-406. doi:

10.1021/acs.nanolett.6b04364. Epub 2016 Dec 23. PMID: 27990827; PMCID:

PMC5423523.

 

7: Liu Z, Bun P, Audugé N, Coppey-Moisan M, Borghi N. Vinculin head-tail

interaction defines multiple early mechanisms for cell substrate rigidity

sensing. Integr Biol (Camb). 2016 Jun 13;8(6):693-703. doi: 10.1039/c5ib00307e.

Epub 2016 May 11. PMID: 27169142.

 

8: Lowndes M, Rakshit S, Shafraz O, Borghi N, Harmon RM, Green KJ, Sivasankar S,

Nelson WJ. Different roles of cadherins in the assembly and structural integrity

of the desmosome complex. J Cell Sci. 2014 May 15;127(Pt 10):2339-50. doi:

10.1242/jcs.146316. Epub 2014 Mar 7. PMID: 24610950; PMCID: PMC4021477.

 

9: Borghi N, Sorokina M, Shcherbakova OG, Weis WI, Pruitt BL, Nelson WJ, and Alexander R. Dunn

E-cadherin is under constitutive actomyosin-generated tension that is

increased at cell-cell contacts upon externally applied stretch. Proc Natl Acad

Sci U S A. 2012 Jul 31;109(31):12568-73. doi: 10.1073/pnas.1204390109. Epub 2012

Jul 16. Erratum in: Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):19034. PMID:

22802638; PMCID: PMC3411997.

 

10: Borghi N, Lowndes M, Maruthamuthu V, Gardel ML, Nelson WJ. Regulation of

cell motile behavior by crosstalk between cadherin- and integrin-mediated

adhesions. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13324-9. doi:

10.1073/pnas.1002662107. Epub 2010 Jun 21. PMID: 20566866; PMCID: PMC2922157.