Epigenome and Paleogenome>> Paleoepigenomics and the evolution of regulatory sequences

We develop the study of epigenomic modifications of the epigenome (methylation of DNA) in ancient samples to measure on the witnesses of evolution the functional impact of sequence changes prone to induce modifications of gene expression.
Methylation of DNA in ancient samples can be studied via a combination of classical approaches: sequencing after bisulfite treatment and the analysis of cytosine deamination occurring in ancient samples. Methylated deaminated cytosines can be discriminated from non-methylated cytosines using uracil-N-glycosylase. By doing so, we will study the evolution of methylation patterns of selected genes in mammoths and elephants, but also in other evolutionary cases, such as bovids and humans.

DNA methylation will be studied as a proxy of gene expression. The aim is to correlate modifications of regulatory sequences with changes of gene expression to study the evolution of the regulation of gene expression, which is a motor of phenotypic evolution.

Go to top page