Complexes macromoléculaires en cellules vivantes
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Gradient of mechanical tensions and subcellular asymetries establishment.
Sommaire
Concentration differences of a few per cent across a cell or a growth cone can be sufficient for their chemotactic orientation. However, for mechanical stimuli, the amplitude of the force gradient which would sustains the induction (and/or the stabilization) of subcellular asymmetry is not known. Is the intensity range of applied forces important or is the geometrical shape and asymmety of the gradient critical for the dynamical balance of regionalization processes'
What is the effect of the force gradient on cytosqueleton dynamics, Rho-GTPase activation and polarized transport' To answer these questions, our project is to combine nanomanipulation (in 2D and 3D) systems in order to apply controled forces at specific cellular location and real time two-photon fluorescence lifetime imaging microscopy to follow the subcellular dynamics of molecular events downstream to integrin activation.
Fibronectin-coated beads are trapped by the multile opitical tweezers and positioned on the cortex of a round cell. Rigidity gradient can be applied on the cell cortex . In e) the cell nucleus is visualized by Hoechst fluorescence.
Dernière modification 14/03/2011