ImagoSeine : cytométrie en flux, imagerie photonique, microscopie électronique
Overview:
ImagoSeine bioimaging is a core facility which provide access to state of the art equipments as well as provide training and continue the development of imaging methods and technologies.
The objectives are to maintain the imaging technology development at the top, to give to external users the possibility to perform biological research at a competitive international level, to transfer methodologies and technologies to scientific and medical community.
The ultimate goal is to visualize single biomolecules and quantify their functions, dynamics and interactions within the context of live biological systems (cells and organisms).
The core facility ImagoSeine has been assessed and found to meet the requirements of ISO 9001 by AFNOR Certification. The certificate is valid from 2009-12-30 until 2012-12-29.
Competences:
Visualization in depth in living samples (macromolecules and cell migration/division), visualization at high spatial resolution (ultra structural immuno localization analysis, sub cellular traffic), high throughput for biology systems, manipulation (cell sorting, photo and nano manipulations), functional imaging of live cells (dynamics quantification of macromolecular interactions and biochemical reactions, morphogenetic movements,…), image processing and analysis.
Perspectives:
Super-resolution light microscopy, correlative light and electron microscopy, high throughput microscopy.
Resources:
- Flow cytometry cell (or organelle) sorting, cloning and analysis
- 5D Video microscopy combined with photomanipulation
- Spectral Imaging
- Multifocal Monophoton Microscopy combined with photomanipulation
- Multifocal Multiphoton Microscopy combined with FLIM (Fluorescence Lifetime Imaging Microscopy) and FAIM (Fluorescence Anisotropy Imaging Microscopy)
- Fluctuation Spectroscopy Microscopy (FCCS, ccRICS (cross correlation Raster Image Correlation Spectroscopy), ICCS)
- Electron microscopy of cells: morphological characterization andimmunolocalization of biochemically defined antigens
- Multiple optical tweezers
ImagoSeine is supported by IBiSA, ANR PF, FRM, ARC, CNRS, Inserm and Université Paris Diderot.
Supervisor: Maïté Coppey-Moisan
Dernière modification 13/04/2011