Cell Adhesion and Mechanics
Mechanical constraints and force transmission play an essential role in multicellular living organisms, regulating basic biological processes such as morphogenesis, tumor metastasis and tissue repair. Cell adhesions, coupled to the contractile cytoskeleton, are major sites of force transmission in cells. This mechanical coupling which enables cells to sense, signal, and respond to physical changes in the environment, has however been largely understudied. Our research aims at understanding how cell adhesion-associated mechanotransduction and mechanosensing regulates cell behavior and tissue mechanics. In this context, we are studying the cooperation between adhesion, mechanical and biochemical signaling for the adaptation of living cells to changes in their physical environment at various scales, from single molecules to tissues.
Research areas (mechanics and mechanosensing, from adhesion complexes, to single cells , cell assemblies and tissues)
The overall goal of the team is to understand how coordinated regulation of cell-substrate and cell-cell adhesion and cytoskeletal dynamics contributes to cell and tissue mechanics and adapted cell response to forces, particularly in the context of cell adhesion to substrates of controlled stiffness and geometry and the dynamics of epithelial sheets (open or confined collective migration, wound repair, apoptotic cell extrusion, skin and intestinal epithelium homeostasis and associated pathologies).
Epithelial cell migration; Microfabrication for cell mechanics; Influence of the mechanical environments on cell functions; Adherens junctions; Cadherins; Mechanotransduction; Molecular mechanosensors; Collective cell migration; Cell polarity
Sélection of publications
Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, Mayeux A, Michaux G, Campeotto F, Lemale J, Viala J, Poirier F, Minc N, Schmitz J, Brousse N, Ladoux B, Goulet O, Delacour D. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017 Jan 13;8:13998.
Bertocchi C, Wang Y, Ravasio A, Hara Y, Wu Y, Sailov T, Baird MA, Davidson MW, Zaidel-Bar R, Toyama Y, Ladoux B, Mege RM, Kanchanawong P. Nanoscale architecture of cadherin-based cell adhesions. Nat Cell Biol. 2017 Jan;19(1):28-37.
Kocgozlu L, Saw TB, Le AP, Yow I, Shagirov M, Wong E, Mège RM, Lim CT, Toyama Y, Ladoux B. Epithelial Cell Packing Induces Distinct Modes of Cell Extrusions. Curr Biol. 2016 Nov 7;26(21):2942-2950.
Strale PO, Duchesne L, Peyret G, Montel L, Nguyen T, Png E, Tampé R, Troyanovsky S, Hénon S, Ladoux B, Mège RM. The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity. J Cell Biol. 2015 Jul 20;210(2):333-46.
Gupta M, Sarangi BR, Deschamps J, Nematbakhsh Y, Callan-Jones A, Margadant F, Mège RM, Lim CT, Voituriez R, Ladoux B. Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing. Nat Commun. 2015 Jun 25;6:7525.
Yao M, Qiu W, Liu R, Efremov AK, Cong P, Seddiki R, Payre M, Lim CT, Ladoux B, Mège RM, Yan J. Force-dependent conformational switch of α-catenin controls vinculin binding. Nat Commun. 2014 Jul 31;5:4525.
Vedula SR, Hirata H, Nai MH, Brugués A, Toyama Y, Trepat X, Lim CT, Ladoux B. Epithelial bridges maintain tissue integrity during collective cell migration. Nat Mater. 2014 Jan;13(1):87-96.
Last modified 28 February 2017