ImagoSeine: Flow Cytometry, Electron Microscopy, Imaging

Overview:

ImagoSeine is a core facility that provides access to state-of-the-art equipment for bioimaging, accompanied by training sessions and the development of imaging methods and technologies.
The aims are to develop top-notch imaging technologies, to give users the possibility to perform biological research at a competitive international level, and to transfer methodologies and technologies to the scientific and medical communities.
The ultimate goal is to visualize single biomolecules and quantify their functions, dynamics and interactions within the context of live biological systems (cells and organisms).

ImagoSeine has been assessed by AFNOR Certification and found to meet the requirements of ISO 9001. The certificate is valid until December 29, 2012.

Competences:

Visualization in depth in living samples (macromolecules and cell migration/division), visualization at high spatial resolution (ultra structural immuno localization analysis, sub cellular traffic), high throughput for biological systems, manipulation (cell sorting, photo- and nanomanipulations), functional imaging of live cells (dynamic quantification of macromolecular interactions and biochemical reactions, morphogenetic movements,…), image processing and analysis.

Perspectives:

Super-resolution light microscopy, correlative light and electron  microscopy, high throughput microscopy.

Resources:

  • Flow cytometry cell (or organelle) sorting, cloning and analysis
  • 5D Video microscopy combined with photomanipulation
  • Spectral Imaging
  • Multifocal Monophoton Microscopy combined with photomanipulation
  • Multifocal Multiphoton Microscopy combined with FLIM (Fluorescence Lifetime Imaging Microscopy) and FAIM (Fluorescence Anisotropy Imaging Microscopy)
  • Fluctuation Spectroscopy Microscopy (FCCS, ccRICS (cross correlation Raster Image Correlation Spectroscopy), ICCS)
  • Electron microscopy of cells: morphological characterization andimmunolocalization of biochemically defined antigens
  • Multiple optical tweezers


ImagoSeine is supported by IBiSA, ANR PF, FRM, ARC, CNRS, Inserm and Université Paris Diderot.

Supervisor: Maïté Coppey-Moisan

Last modified 04/13/2011

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