Genomics-Transcriptomics
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Real-time PCR
The Light Cycler 1.5 allows the reliable, sensitive and specific quantification of nucleic acid sequences, the characterisation of PCR products by melting curve analysis and genotyping of SNP in less than one hour. It is possible to analyse up to 32 20-µl glass capillaries simultaneously . Sample excitation can be achieved with a maximum emission at 470 nm, and fluorescence can be measured simultaneously at 530, 640, and 705 nm. Measurements can be done with SyBr Green I, specific probes (Taqman, Scorpion, Beacon, etc…) labelled with FAM, and hybridization probes labelled with the couples FAM-LightCycler Red 640 or 705. Multiplexing is not possible except in the hybridization probe format.
For a more detailed description, see www.roche-applied-science.com/sis/rtpcr/lightcycler/
These machines are used as a self-service and you will require prior training by one of the members of your team. For new teams arriving at the Institute, this training will be offered by Thierry Grange (thierry.grange(at)univ-paris-diderot.fr) or a member of his team.
The light cyclers are very busy. You must therefore reserve in advance using the notebook available by each LightCycler. Unless you need to use a lot of cycles (>50), it is mandatory to reserve - at the most - a 90 minute session and to respect it. Your samples should normally be prepared before you start your session.
Microarray reader Genepix
The Genepix 4000B can scan DNA microarrays (microscopic slide) at 532 et 635 nm with a 5 µm-resolution. It is driven by the GenePix Pro software. It is used on a self-service basis, but its use requires prior arrangement with Thierry Grange to ensure coordination between the different users. If many analyses are planned, it might be necessary for your team to purchase a licence for the installation of the GenePix Pro software on one of your lab computers. The computer driving the microarray reader also drives the microspetrophotometer Nanodrop, and you must give access to Nanodrop users when you are performing data analysis.
For more information, see www.moleculardevices.com/pages/instruments/gn_genepix4000.html
Microspectrophotometre Nanodrop ND1000
The Nanodrop ND1000 allows acquisition of absorbance spectra between 220 and 750 nm for 1µl samples. It is used on a self-service basis after initial training by a member of your team. For new teams, training will be offered by Thierry Grange (thierry.grange(at)univ-paris-diderot.fr) or a member of his team.
For more information, see www.nanodrop.com/nd-1000-overview.html
Last modified 03/14/2011