Genomics-Transcriptomics

This facility is equipped with two real-time PCR machines Light Cycler 1.5 Roche, one Axon GenePix 4000B microarray reader and one Nanodrop microspectrophotometer.
Real-time PCR allows the reliable, sensitive and specific quantification of nucleic acid sequences, the characterisation of PCR products by melting curve analysis and genotyping of single nucleotide polymorphism (SNP) in less than one hour.

Amplification curve

 

Melting peaks

 

Melting peaks

With DNA chips it is possible to measure in a single step the changes in relative abundance of a large number of nucleic acid sequences.
They can be used to quantify the mRNAs expressed in cells, to analyse the distribution of chromosomal proteins along the genome, and to identify the amplification and deletions of chromosomal regions as well as genetic polymorphisms.

 

The microspectrophotometer can be used to acquire the absorbance spectra of very small samples (1 µl).

Scientific supervisor: Thierry Grange, CNRS

Last modified 01/27/2012

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